| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 4,586,030 | 2 bp→TC | intergenic (+113/+101) | PP_4061 → / ← PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,586,030 | 0 | C | T | 96.3% | 72.6 / NA | 27 | intergenic (+113/+102) | PP_4061/PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (9/17); minor base A (1/0); total (10/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 4,586,031 | 0 | T | C | 96.3% | 79.0 / ‑6.1 | 27 | intergenic (+114/+101) | PP_4061/PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (9/17); minor base G (1/0); total (10/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCA‑GGGA‑C‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCG > NC_002947/4585887‑4586139 || gccgAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑gg < 8:29881/149‑1 (MQ=255)gccgAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑gg < 8:184467/149‑1 (MQ=255) cGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGATAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTAcc < 2:64282/149‑1 (MQ=255) ttAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑ggggcagcg > 3:434254/1‑143 (MQ=38) ggTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTcc < 1:406076/130‑1 (MQ=255) ggTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTcc > 2:406076/1‑130 (MQ=255) gACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTTGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCtt < 8:182651/148‑1 (MQ=38) cACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑t > 6:146117/1‑149 (MQ=39) aCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTgg < 7:132655/149‑1 (MQ=39) aTCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGa < 2:301549/147‑1 (MQ=39) tGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCATGGCGGGTAAAACCCCAACAAACA‑GGGACC‑CTGCC‑C‑GCCAAGGA‑AC‑CGGCGCAGCCCCAGGGAGGCGCggg > 6:239176/1‑149 (MQ=255) gcgcTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCggg > 8:157958/1‑148 (MQ=38) gcgcTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCggg < 7:157958/148‑1 (MQ=38) cgcTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGG‑CAGCGCC‑‑‑GCATTCAA‑GGCCGGCACGGTCCCTGGTAGGAGCGGGt < 4:434254/148‑1 (MQ=14) ctCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAACTG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTAc < 1:52811/148‑1 (MQ=37) cATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCcgcg < 5:146117/149‑1 (MQ=38) ttGAAAGGTTGAGGATGACCGGTTGCTTGGCAAGGTGAAGATCTTGCATGGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGaa < 8:302248/149‑1 (MQ=37) gAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTAc > 6:159663/1‑86 (MQ=37) gAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTAc < 5:159663/86‑1 (MQ=37) aaaGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCGGCCCCTGGTAGGAGCGGGTTTACCCGCGAaga < 5:305045/148‑1 (MQ=37) gATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCggg > 6:362630/1‑124 (MQ=37) gATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCggg < 5:362630/124‑1 (MQ=37) cggggTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑ggg < 6:108448/73‑1 (MQ=21) ccGGGTCCTTGGCAAGGTGAAGATCTTGCATTCATCGCGCCGGCCTCTACGCGGGTAAACCCGCTCCTACCA‑ggg > 5:108448/1‑75 (MQ=18) gAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTTAAACCCCGCCGACCACGGGC‑CGGGGCCTGTGCCGTGAA‑TG‑CGGCGCTGCCCCGGGTAGGAGCGGGTTTACCCGCGAAGCGGCCGGCCGCGCCAGCACATCAACCCg > 1:350018/1‑145 (MQ=16) aTCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGc > 2:429073/1‑70 (MQ=17) aTCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGACC‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGc < 1:429073/70‑1 (MQ=17) || GCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCA‑GGGA‑C‑GTGCC‑G‑GCCTTGAA‑TG‑CGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCG > NC_002947/4585887‑4586139 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |