mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | ALE-49_S49_L001_R1_001.good.fq | 439,829 | 61,791,671 | 100.0% | 140.5 bases | 149 bases | 94.5% |
| errors | ALE-49_S49_L001_R2_001.good.fq | 439,829 | 61,505,686 | 100.0% | 139.8 bases | 149 bases | 95.3% |
| errors | ALE-49_S49_L002_R1_001.good.fq | 436,805 | 61,223,238 | 100.0% | 140.2 bases | 149 bases | 95.9% |
| errors | ALE-49_S49_L002_R2_001.good.fq | 436,805 | 61,016,898 | 100.0% | 139.7 bases | 149 bases | 95.9% |
| errors | ALE-49_S49_L003_R1_001.good.fq | 447,953 | 62,743,790 | 100.0% | 140.1 bases | 149 bases | 95.8% |
| errors | ALE-49_S49_L003_R2_001.good.fq | 447,953 | 62,489,383 | 100.0% | 139.5 bases | 149 bases | 96.4% |
| errors | ALE-49_S49_L004_R1_001.good.fq | 418,841 | 58,676,851 | 100.0% | 140.1 bases | 149 bases | 95.8% |
| errors | ALE-49_S49_L004_R2_001.good.fq | 418,841 | 58,476,971 | 100.0% | 139.6 bases | 149 bases | 95.6% |
| total | 3,486,856 | 487,924,488 | 100.0% | 139.9 bases | 149 bases | 95.7% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_002947 | 6,181,873 | 75.7 | 2.2 | 100.0% | Pseudomonas putida KT2440 chromosome, complete genome. |
| total | 6,181,873 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 65826 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 579 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.029 |
| reference sequence | pr(no read start) |
|---|---|
| NC_002947 | 0.78296 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.3.4.1 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 02:37:07 19 Feb 2020 | 02:37:58 19 Feb 2020 | 51 seconds |
| Read alignment to reference genome | 02:37:58 19 Feb 2020 | 02:45:18 19 Feb 2020 | 7 minutes 20 seconds |
| Preprocessing alignments for candidate junction identification | 02:45:18 19 Feb 2020 | 02:46:06 19 Feb 2020 | 48 seconds |
| Preliminary analysis of coverage distribution | 02:46:06 19 Feb 2020 | 02:48:03 19 Feb 2020 | 1 minute 57 seconds |
| Identifying junction candidates | 02:48:03 19 Feb 2020 | 03:04:10 19 Feb 2020 | 16 minutes 7 seconds |
| Re-alignment to junction candidates | 03:04:10 19 Feb 2020 | 03:06:36 19 Feb 2020 | 2 minutes 26 seconds |
| Resolving best read alignments | 03:06:36 19 Feb 2020 | 03:07:51 19 Feb 2020 | 1 minute 15 seconds |
| Creating BAM files | 03:07:51 19 Feb 2020 | 03:09:39 19 Feb 2020 | 1 minute 48 seconds |
| Tabulating error counts | 03:09:39 19 Feb 2020 | 03:10:15 19 Feb 2020 | 36 seconds |
| Re-calibrating base error rates | 03:10:15 19 Feb 2020 | 03:10:18 19 Feb 2020 | 3 seconds |
| Examining read alignment evidence | 03:10:18 19 Feb 2020 | 03:17:12 19 Feb 2020 | 6 minutes 54 seconds |
| Polymorphism statistics | 03:17:12 19 Feb 2020 | 03:17:12 19 Feb 2020 | 0 seconds |
| Output | 03:17:12 19 Feb 2020 | 03:17:49 19 Feb 2020 | 37 seconds |
| Total | 40 minutes 42 seconds | ||
