Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,499,508	+C	intergenic (+85/‑79)	trpS → / → zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,499,506	1	.	C	100.0%	116.3 / NA	40	intergenic (+83/‑81)	trpS/zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (22/18);  total (22/18)

TGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGGCACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCG  >  NC_002947/1499363‑1499649                                                                                                                                                   |                                                                                                                                                tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCAcg‑cccg                                                                                                                                             <  6:372278/149‑3 (MQ=25)      cccGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTcc                                                                                                                                          >  6:380752/1‑148 (MQ=25)        cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCca                                                                                                                                        >  5:176135/1‑148 (MQ=25)              gCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACt                                                                                                                                  >  1:99386/1‑148 (MQ=21)                         gagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCAcg‑c                                                                                                                                                <  7:323378/122‑1 (MQ=25)                          agCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAg                                                                                                                     <  2:71962/149‑1 (MQ=21)                          agCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCACGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAg                                                                                                                     >  2:84167/1‑149 (MQ=21)                            ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCg                                                                                                                   >  7:391893/1‑149 (MQ=21)                             cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTTGCCGCGAAACAGGCGCCCCGGGGGATGGCACCGGCTTTGCCGGTGT‑TAGCGGGCCCGCCCGCGCCCACCGACGGATTGCCCATAAGCgg                                                                                                                  >  6:371143/1‑149 (MQ=21)                             cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCCGCTTTGCCGGTGT‑TAGCGGGCACGCCCGCTCCCCCAGACTGATTGACATTAAGccg                                                                                                                  >  6:180971/1‑147 (MQ=21)                              tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTccc                                                                                                                                         <  8:321674/125‑1 (MQ=18)                              tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTccc                                                                                                                                         >  7:321674/1‑125 (MQ=18)                                   gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTAt                                                                                                            <  6:385808/149‑1 (MQ=21)                                    aaGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAGGGTGGGAGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCACGGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATc                                                                                                           <  1:373981/149‑1 (MQ=21)                                        cgcgccaccacAGCAGGACGGCGGCCGCCAAACGGTGGGCGCGGGCGCGCCCGCGAACCAGCCGCCGCGGTGCATGCCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTa                                                                                                       <  4:281083/144‑1 (MQ=18)                                           ggCACCACAGCAGGACTTATGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACa                                                                                                     <  6:289165/148‑1 (MQ=21)                                             caccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTg                                                                                                  <  7:392159/149‑1 (MQ=21)                                               ccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCCCGAAACAGGCGCCGCGGTGCAAGGCACCGGCGTTGCCCGGGT‑TAGCCGGCACGCCCGCGCCCCCAGACTGATTTGCAATAAGCGGGCCGATAGCTTCAgatcg                                                                                                >  5:154936/1‑145 (MQ=255)                                               caacaGGAGGACTGTGGCTGCCAACCGGGGGTAGCGGGCGGGCCCGCTAAACTGGCGCGGCGGTGCATGGCACCGGGTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTa                                                                                                       <  6:154936/140‑1 (MQ=18)                                                   agcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGcc                                                                                            >  7:90077/1‑149 (MQ=21)                                                        gACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCCCAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGttt                                                                                       >  6:206039/1‑149 (MQ=21)                                                              ggCTGCCAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                                                                 <  8:391893/149‑1 (MQ=21)                                                               gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                                                                 <  8:90077/148‑1 (MQ=21)                                                                                 ggggCTTGCCCGCTAAACAGGCGCCGCGGTGATTGCCCCCGGGTTTGCCGGTGTGC‑GCGGCCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATg                                                              <  3:175410/148‑1 (MQ=255)                                                                                   ggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGAc                                                            <  5:206039/149‑1 (MQ=25)                                                                                          ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                                                       >  8:107402/1‑147 (MQ=37)                                                                                          ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa                                                     <  5:371143/149‑1 (MQ=37)                                                                                                   aGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt                                            >  1:278027/1‑149 (MQ=37)                                                                                                    ggCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCACTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt                                            >  4:173998/1‑148 (MQ=37)                                                                                                       gccgcGGTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCGTGACTCCCTTAGAACGCTATCAAg                                        <  5:380752/149‑1 (MQ=37)                                                                                                             gTGCATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATc                                  <  4:57862/149‑1 (MQ=38)                                                                                                                cATGGCACCGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg                                >  4:298575/1‑148 (MQ=38)                                                                                                                     caccGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGt                          <  3:378440/146‑1 (MQ=38)                                                                                                                      accGGCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTc                         >  4:401462/3‑149 (MQ=38)                                                                                                                         ggCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTccc                       >  4:75793/1‑148 (MQ=39)                                                                                                                          gCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGa                     >  7:1088/1‑149 (MQ=39)                                                                                                                          gCTTTGCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGa                     <  5:180971/149‑1 (MQ=39)                                                                                                                               gCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGa                               <  3:336887/134‑1 (MQ=39)                                                                                                                               gCCGGTGT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGa                               >  4:336887/1‑134 (MQ=39)                                                                                                                                    gtgttcgcGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTGTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCGGAAACGTCCAGCCTGCTTCCa            >  8:288090/8‑149 (MQ=255)                                                                                                                                            gggCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACgcgg    >  6:85578/1‑149 (MQ=255)                                                                                                                                              gCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCAATGACgcggcg  >  8:252042/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                TGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑TCGGGCACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCG  >  NC_002947/1499363‑1499649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑