| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 97.9% | 150.9 / ‑3.6 | 47 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (25/21); total (25/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACA > NC_002947/1932495‑1932776 | ggcgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTG‑cccgc < 5:152990/149‑4 (MQ=255) tCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTACCCGCGGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGCAGCGGG‑CTTGCCCGCGAAg < 3:24515/149‑1 (MQ=38) cGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGGCGCACGAAAATCGCAGTAAACGCTGGGGCCGCTTTGCGGCCTTTCGCGGGCAAGCCCGCTCCTACAAAGCATATGCGGCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAggg < 3:126526/147‑1 (MQ=37) gagGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGc > 6:181172/1‑147 (MQ=255) gATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAgg > 5:138045/1‑139 (MQ=255) aTTGGACAGCGGCATGGCAGGTCTCACGAAAATAGCAGTAAAGGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCATACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCaaa < 8:279857/149‑1 (MQ=37) ccAGCGGCAGTGCCGGGCTCACGAAAATCGCAGTACCCGCGGGGGCCGCTTTGCGGCCCTGCGCGGGCAAGCCCGCGCCTACAAAGGCTATGCGGCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 7:377101/148‑1 (MQ=37) aCAGCGGCATTGCAGGTCTCACCAAAATAGCAGCACACGCTGGGGCCGCTTTGCGGCCCTGCGCGGGCAAGCCCGCTCCTACAAAGGATATGCCTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 5:134703/149‑1 (MQ=38) cGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACt < 4:314912/149‑1 (MQ=255) cGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGCATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACt < 5:181172/149‑1 (MQ=255) cGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTACTACAAAGGATATGCGTCCCCGGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAc > 3:168271/1‑148 (MQ=255) aTTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTG‑c < 3:337354/108‑1 (MQ=255) aTTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTG‑c > 4:337354/1‑108 (MQ=255) aTTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCGGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTAcgc > 7:88060/1‑149 (MQ=255) gCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGa > 5:108074/1‑149 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATc > 6:305312/1‑149 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATc > 4:261367/1‑149 (MQ=255) gAAAATAGCTTTAAACGGGGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGTATTTGCGGCCCCTGTAGGTGCGGG‑GTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCg < 6:240555/149‑1 (MQ=25) aaTAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCa < 3:261367/149‑1 (MQ=255) gTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGAt > 5:299662/1‑149 (MQ=255) aCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTAcgc > 6:384765/1‑121 (MQ=255) aCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTAcgc < 5:384765/121‑1 (MQ=255) gggCCGCTTTGCGGCCCTTCGCGGGCACGCCCGCTCCTACAATGGATTTGCGTCCCCTGTTGGTGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTcc < 8:88060/149‑1 (MQ=38) ccGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGGAAGGGAGCCCCAAAATGTCGACTACGCGGATCAGGGATCCGGCAgg > 7:381372/1‑131 (MQ=37) ccGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAg > 1:264517/1‑148 (MQ=255) cGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGaa > 5:86630/1‑149 (MQ=255) gCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGag > 2:292990/1‑148 (MQ=255) ggCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagcag < 6:86630/149‑1 (MQ=255) ggCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagcag > 1:380593/1‑138 (MQ=255) ggCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagcag < 2:380593/138‑1 (MQ=255) gCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGttt < 5:305312/149‑1 (MQ=255) aaGCCCGGTCCTACAAAGGATAGGCGTCCCGTGTTGGTGCGGG‑GTGGCCCGCGAAGGGCTGCAAGGCAGCCCCAATTTGTCGACTACGCGGATCAGCGATGCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc < 3:368064/149‑1 (MQ=255) cccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAg > 8:9552/1‑149 (MQ=255) gCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCACCAAATTGTCGAATACGCGGATCAGCGATCCGCCAGGGTGATGTTCATCTCCAGAATCGAGCAGCTGCCCTGGTTTTc > 7:333859/1‑143 (MQ=39) tCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGa > 1:369956/1‑149 (MQ=255) cTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGata < 7:236111/149‑1 (MQ=255) aCATAGGATATGGGTAAGCTTTAGGAGCGGGTTTTG‑CCTCGAAGGGCTGCAAGGCAGCCACATATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTAAGCTCCAGAATCGAGAAGCTGGCCTGGTTTTCCAGCTCGat < 8:333859/146‑1 (MQ=255) cAAAGTATATGCGTCCCCTTTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCTCTGGTTTTCCAGCTCGATATGc < 7:61430/149‑1 (MQ=39) cAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGTCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGc < 2:264517/149‑1 (MQ=255) cAAAGGATATGCGTCCCCTGGTTGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGc < 6:289136/149‑1 (MQ=39) aaaGGATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGAGGTTCAGCTCCAGAATCGCGCAGCTGCCCTGGTTTTCCCGCTCGATATGcc > 5:408614/1‑148 (MQ=39) gATATGCGTCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatc < 1:292990/149‑1 (MQ=255) tCCCCTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGc > 2:329829/1‑149 (MQ=255) ccccTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 2:294439/54‑1 (MQ=17) ccccTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc > 1:294439/1‑54 (MQ=17) cccTGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGcc > 3:189686/1‑148 (MQ=255) tGTAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATAAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATg > 1:32048/1‑149 (MQ=255) tAGGAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGt > 8:173966/1‑148 (MQ=255) gAGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTca > 3:357906/1‑148 (MQ=255) aGCGGG‑CTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTcaca > 8:161240/1‑149 (MQ=255) | GGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGG‑CTTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACA > NC_002947/1932495‑1932776 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |