| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 3,845,705 | +G | intergenic (‑72/+26) | PP_3394 ← / ← PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,845,703 | 1 | . | G | 94.2% | 166.8 / 3.0 | 52 | intergenic (‑70/+28) | PP_3394/PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base G (29/20); total (31/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑C‑GG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAAT > NC_002947/3845557‑3845833 | gctgctGTCGTCATGATCACTTGCAGGCTCTGCAAGCCATCACGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTc‑g‑c‑g < 5:204158/148‑1 (MQ=255) tgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGAATGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCTCAAGACTAATAGCTGGATGACTGGCAGAAAAGAAGTGTTTA‑G‑C‑gggc > 3:281450/1‑147 (MQ=255) tgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑C‑gggctga > 2:1588/1‑144 (MQ=255) gTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGAt > 6:399708/1‑149 (MQ=255) gCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATGCAt > 1:194758/1‑149 (MQ=255) cACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGc > 2:145051/1‑149 (MQ=255) ttGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCa > 6:348939/1‑144 (MQ=255) ttGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCa < 5:348939/144‑1 (MQ=255) gCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGa > 5:77854/1‑149 (MQ=255) cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGAt < 6:77854/149‑1 (MQ=255) ctctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGGAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGAt < 1:297383/149‑1 (MQ=255) ctctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCagcagcag > 4:369784/1‑141 (MQ=255) ctctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGAt < 7:70387/149‑1 (MQ=255) cAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTGGCTGACGATGGATCAGc < 4:252065/148‑1 (MQ=39) aGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGAt > 1:162957/1‑142 (MQ=255) ccATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTAGGGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCcgc > 1:122480/1‑149 (MQ=255) ccATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGa < 4:6611/139‑1 (MQ=255) ccATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGa > 3:6611/1‑139 (MQ=255) cATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGACGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCgcgc < 3:73000/149‑1 (MQ=255) cATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCgcgc > 6:384076/1‑149 (MQ=255) gCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGGGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GAG‑GG‑CTGATACATCGTCAGCCACGATGGATCATCCCGCGCCCTGCCAGGc > 8:43380/1‑149 (MQ=255) cGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCg > 1:15982/1‑149 (MQ=255) aCTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGAACCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGt < 1:290490/149‑1 (MQ=255) attgttttttttGCCTGCGGGGTCTCTGTGGCTTACGGGTG‑GCGCCAGACTATCTGCTGTATGCGGGGCATAAAAGCCGTGTTTCGG‑G‑GG‑CTGACCCATCGCCCCCCACGTTGTATCAGCCCGCGCCCTGCCAGGCCGGTGCACgcag < 2:343225/148‑1 (MQ=255) attgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGAACCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACgcag < 1:1588/148‑1 (MQ=255) attgttGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAgg > 1:22688/1‑149 (MQ=255) ttgtCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggtggt > 3:81146/1‑149 (MQ=255) ttgtCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggtggt > 7:130897/1‑149 (MQ=255) tCATCGCGGTATCTCTGTCTCAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑C‑GGCCTGATCCATCGTCAGCCACGAAGGATCAGCCCGAGACCTGCAAGGCCAGTCCACGCAGCAACAGGTGGTCa > 8:245166/1‑148 (MQ=255) aTCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGc < 5:399708/149‑1 (MQ=255) tctgtctgAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCCCGCAGCAACAGGTGGTCAAGCAGGCgccgagc > 7:30567/1‑149 (MQ=255) tctgtctgAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgagc > 1:142407/1‑149 (MQ=255) tctgtctgAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTAAAGCAGGCgccgagc > 3:347870/1‑149 (MQ=255) gctgAAGCGTT‑GCGCCAGACTAAGAGGTGGATGACGGGCACAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATGGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgagcc < 8:130897/145‑1 (MQ=255) cttaaGGGTT‑GCGCCAAAATAAGAGCTGTTTGAGGGGAAAAAAAGAGGTGTTTC‑G‑CGGG‑GTTATCCATCGTCACCAACTTTGTATCTGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGAc < 2:22688/145‑1 (MQ=255) cGTT‑GCGCCATACTAAGAGCTGGATGACGGGCATAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACTATGTATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCgaga < 2:122480/149‑1 (MQ=255) gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGAt > 4:27736/1‑52 (MQ=255) gcCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGAt < 3:27736/52‑1 (MQ=255) ccAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGtgtg > 5:215199/1‑149 (MQ=255) gACTAAGAGCTGGATGACGGGCAGAAAAGACGGGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCg < 8:306549/149‑1 (MQ=255) agagCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTcgc > 8:51547/1‑149 (MQ=255) gATGACGGGCATAAAACACGTGTTCC‑G‑CGGG‑CGTACCCATCGCCCCCCCCTATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATg < 7:43380/147‑1 (MQ=255) gATGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATg < 5:384076/147‑1 (MQ=255) tGACGGGCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCga > 3:213672/1‑123 (MQ=255) gggCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGAc > 5:39398/1‑149 (MQ=255) gggCAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGAc > 2:10614/1‑149 (MQ=255) cAGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGaa > 5:228565/1‑149 (MQ=255) aGAAAAGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTTCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCCACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAAt > 5:355557/1‑149 (MQ=255) aaaaGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGAt > 7:200628/1‑140 (MQ=255) aaaaGACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGAt < 8:200628/140‑1 (MQ=255) gACGTGTTTC‑G‑CGGG‑CTTATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAgg < 3:343217/92‑1 (MQ=255) gACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGTTCAGCCCGCGCCCTGCCAGGCCGGTGGACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGc > 5:275723/1‑119 (MQ=255) gACGTGTTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAgg > 4:343217/1‑92 (MQ=255) ttttC‑G‑CGGG‑CTGATCCATCGTCAGCCACTTTGTATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggtggt < 1:23138/78‑1 (MQ=255) gtTTC‑G‑CGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggtggt > 2:23138/1‑79 (MQ=255) | GCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTT‑GCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑G‑C‑GG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAAT > NC_002947/3845557‑3845833 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |