Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_002947	5,681,415	+CGGG	intergenic (‑133/+71)	PP_4986 ← / ← PP_4987	hemolysin III family channel protein/putative Chemotaxis protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 5681415	2 (0.030)	36 (0.530) +CGGG	30/272	2.2	94.9%	intergenic (‑133/+71)	PP_4986/PP_4987	hemolysin III family channel protein/putative Chemotaxis protein
	?	NC_002947	5681416 =	2 (0.030)					intergenic (‑134/+70)	PP_4986/PP_4987	hemolysin III family channel protein/putative Chemotaxis protein

GTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/5681274‑5681415 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGC  >  NC_002947/5681416‑5681555                                                                                                                                                ||||                                                                                                                                             GTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTGGCGAAA‑GGGGCGAGAAGCGGCACCAGGCTTTAATTGCCAATGCATGAAACGCCGGGGCT                                                                                                                                           >  5:412301/1‑149   AATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGG                                                                                                                                               >  4:233533/1‑143   AATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGG                                                                                                                                               <  3:233533/143‑1      ACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGGCGCGAAGCGGCCCCCGGCTTTCATTGCCCATGCATGAACCGCCGGGGCTGCTGC                                                                                                                                      >  6:295182/1‑149        ATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGC                                                                                                                                    <  7:94652/149‑1         TGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGG                                                                                                                                             <  3:40371/139‑1         TGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGG                                                                                                                                             >  4:40371/1‑139            AAACACCGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGTAA‑GGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCC                                                                                                                                <  2:30481/149‑1                ACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTAT                                                                                                                            <  4:166608/149‑1                    ACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTTTGTAGCGAAA‑GGGGCGCGAAGCGGCCCCAGGCTTTTATTGCCACTGCATTAAACGCCGGGGCTGCTGGGGAGCCCTATacc                                                                                                                         >  3:161948/1‑145                    ACCTCCTGAGGTGGGCTGGGTTCCTGGTGTGATGCTGATGGCTTCGTGCTTTGTGGGAGCGGCCTTGTGTGGCGAAA‑GGGGCGCGAAGCGGCCCCAGGCTTTCATTTCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCG                                                                                                                        <  5:294706/149‑1                              GTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCC                                                                                                              >  4:338049/1‑149                                   CTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGG                                                                                                                                              >  5:220790/1‑112                                   CTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGG                                                                                                                                              <  6:220790/112‑1                                            TGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGC                                                                                                                         <  1:248608/125‑1                                            TGTTGTGATGCTGAGGGCTGCGTTCTTTGTGGGAGCGGCCTGGGGTCGCGAAA‑GGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGACCCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCA                                                                                                  <  7:377712/147‑1                                             GTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGGCGCGAAGCGGCCCCAGGCTTTAATTGCCACTGAATGAACCGCCGGGGCTGCTGCGCAACCCAATCGCGAAACAAAGCCCCAACCACACCAGAC                                                                                               >  1:126216/1‑149                                                              TTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGCCACAAGGCCGCGCCCCCTCCAGACCCCCCGGCCCGGCGCCT                                                                              >  7:114011/1‑149                                                                 GTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCA                                                                            >  1:92652/1‑148                                                                         TGGGAGCGGCCTTGTGTCCCTAAA‑GGGGCGCGAAGCGGCCCCAGGCTTTCATTCCCACTCCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCTACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAAC                                                                   <  7:310137/149‑1                                                                              GCGGCCTTGTGTCGCGAAA‑GGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGCCACAAGGCCGCTCCCACTCCCGACC                                                                                              >  3:235484/1‑117                                                                              GCGGCCTTGTGTCGCGAAA‑GGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGG                                                              >  5:206495/1‑149                                                                              GCGGCCTTGGGGCGCTAAC‑GGGGCGCTAAGCGGCCCCAGGCTTTCATTGCCACTGCATCAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACC                                                                                              <  4:235484/117‑1                                                                               CGGCCTTGTGTCGCGAAA‑GGGGCGCGAAGCGGCCACAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGT                                                             >  1:55211/1‑149                                                                                      GTGTCGCGAAA‑GGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGG                                                         <  1:177866/146‑1                                                                                               AA‑GGGGCGCGAAGCGACCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGC                                             <  7:348971/149‑1                                                                                                   GGGCGCTAAGCGCCCCCAGGGTTTCATTGCCACTCAATTAACCGCGGGGGGTGCTGCGCAGCCCTATCGCGACACATGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTC                                          <  1:220261/149‑1                                                                                                           AGCGGCCCCTGGATTTCATTGCCACTGCTTGAACCGCGGGGGCTGCTGCGATGCCTTATAGGGACAAATGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACga                                  <  6:412301/149‑3                                                                                                                 CCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTT                            >  8:291688/1‑149                                                                                                                 CCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTT                             >  8:286176/1‑148                                                                                                                 CCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAA                                                                    >  5:247268/1‑109                                                                                                                 CCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAA                                                                    <  6:247268/109‑1                                                                                                                  cctGGCTTTCATTGCCACTGCATCAACCGCCGGGGGGGCTGCGAAGCCCTACCGCCACAAAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTC                           <  3:131464/146‑1                                                                                                                  CCAGGTTTTCATTGCCACTGCATTAACCGCCGGGGCTGCTGCGAAGCCCTAGCGCTACAAAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTC                           <  8:114011/149‑1                                                                                                                  CCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTC                           >  5:182493/1‑149                                                                                                                       CTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATG                      <  7:286176/149‑1                                                                                                                       CTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATG                      <  7:291688/149‑1                                                                                                                               GCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAG               <  2:55211/148‑1                                                                                                                                          AACCGCCGGGGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCC    <  2:92652/148‑1                                                                                                                                           ACCGCCGCGGCGCCTGCGCCGCCCTATCGCGACACAAGGCCGCGCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGC  <  8:171964/149‑1                                                                                                                                                ||||                                                                                                                                             GTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/5681274‑5681415 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGC  >  NC_002947/5681416‑5681555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.