Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,674,753	G→C	intergenic (+115/+25)	rhlE‑II → / ← yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,674,753	0	G	C	100.0%	218.9 / NA	64	intergenic (+115/+25)	rhlE‑II/yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (30/34);  total (30/34)

CCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGC  >  NC_002947/5674612‑5674890                                                                                                                                                 |                                                                                                                                           ccAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTTCTTTGCAGCCCTATCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCg                                                                                                                                      <  1:136544/149‑1 (MQ=37) ccAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCg                                                                                                                                      <  1:305237/149‑1 (MQ=255)     cgcTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAGCG‑GCCCCCTCGGtttt                                                                                                                                 <  3:169827/149‑1 (MQ=255)       ctgctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGtttt                                                                                                                                 >  1:18606/1‑148 (MQ=255)       ctgctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTc                                                                                                                                <  3:130255/149‑1 (MQ=255)               ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACGAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTgg                                                                                                                          >  3:45760/1‑147 (MQ=255)               ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTgg                                                                                                                          >  8:107415/1‑147 (MQ=255)                     gggCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCAGGGCAGAGAATTTGTTGATATCTAAAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTAAGATGGCAGaaa                                                                                                                    >  1:323509/1‑149 (MQ=25)                       gCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTAc                                                                                                                <  4:267870/149‑1 (MQ=255)                           aTAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTc                                                                                                             <  7:18506/148‑1 (MQ=255)                           aTAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCt                                                                                                            >  5:97024/1‑149 (MQ=255)                              taaGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGc                                                                                                          <  6:65782/147‑1 (MQ=255)                               aaGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGACGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAAGTACTTCTggt                                                                                                         >  8:281850/1‑146 (MQ=37)                                 gTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTca                                                                                                      >  7:175815/1‑149 (MQ=255)                                  tacctcgggggCGGCTTCGCAGCCCTATCACGACACCAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTcac                                                                                                     <  4:104708/143‑1 (MQ=37)                                    ccGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCacac                                                                                                   >  2:112670/1‑149 (MQ=255)                                        gggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                               <  1:295317/149‑1 (MQ=255)                                          gggCTGCTTCGCAGCCCTATCACGACACGAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa                                                                                             <  4:45760/149‑1 (MQ=255)                                          gggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa                                                                                             <  5:169760/149‑1 (MQ=255)                                            gctgctTCGCAGCCCTATCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGaca                                                                                           <  7:107415/149‑1 (MQ=255)                                                ctTCGCAGCCCTATCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTg                                                                                        <  7:386275/148‑1 (MQ=255)                                                ctTCGCAGCCCTATCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGa                                                                                       >  4:346840/1‑149 (MQ=255)                                                 ttCGCAGCCCTATCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGa                                                                                       >  2:207342/1‑148 (MQ=255)                                                     cAGCCCTATCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtc                                                                                   >  6:146867/1‑148 (MQ=255)                                                             tCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccag                                                                          <  2:165679/149‑1 (MQ=255)                                                                cGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag                                                                                >  6:399347/1‑140 (MQ=255)                                                                cGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag                                                                                <  5:399347/140‑1 (MQ=255)                                                                cGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGt                                                                       >  8:78684/1‑149 (MQ=255)                                                                 gACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTc                                                                      >  5:212821/1‑149 (MQ=255)                                                                    acaAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGc                                                                   >  5:194246/1‑149 (MQ=255)                                                                    acaAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGc                                                                   >  3:265076/1‑149 (MQ=255)                                                                    acaAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGc                                                                   >  6:162438/1‑149 (MQ=255)                                                                       aGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGaa                                                                <  4:92609/149‑1 (MQ=255)                                                                        ggCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                         >  3:283582/1‑139 (MQ=255)                                                                        ggCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                         <  4:283582/139‑1 (MQ=255)                                                                          ccGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTa                                                             <  2:18606/149‑1 (MQ=255)                                                                                     cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTTATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCg                                                     <  7:220110/147‑1 (MQ=255)                                                                                       gagaATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGcc                                                 <  5:146867/149‑1 (MQ=255)                                                                                          aaTTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGCCttt                                              <  6:97024/149‑1 (MQ=255)                                                                                           aTTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGCCttt                                              <  5:82481/148‑1 (MQ=255)                                                                                              tCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGCCTTTGAt                                           <  7:78684/148‑1 (MQ=255)                                                                                                     ctctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTa                                                             >  7:246617/1‑124 (MQ=255)                                                                                                     ctctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTa                                                             <  8:246617/124‑1 (MQ=255)                                                                                                       ctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTa                                                             >  1:12880/1‑122 (MQ=255)                                                                                                       ctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTa                                                             <  2:12880/122‑1 (MQ=255)                                                                                                        tACAGGACCGGCCTTGTGTCGTGATAGTGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGCCTTTGATGCCGAAg                                    <  7:281850/145‑1 (MQ=255)                                                                                                         aCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGCCTTTGATGCCGAAGTCgt                                >  3:220234/1‑148 (MQ=255)                                                                                                          cAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGCCTTTGATGCCGAAGTCgttc                              <  6:212821/149‑1 (MQ=255)                                                                                                          cAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGCCTTTGATGCCGAAGTCgttc                              >  1:146105/1‑149 (MQ=255)                                                                                                              aGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGCCTTTGATGCCGAAGTCgttcaggt                          <  6:194246/149‑1 (MQ=255)                                                                                                                 ggCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGCCTTTGATGCCGAAGTCgttcaggttca                       <  2:146105/149‑1 (MQ=255)                                                                                                                  gCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGCCTTTGATGCCGAAGTCgttcaggttcag                      >  7:346295/1‑149 (MQ=255)                                                                                                                       gtgtCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgt                   <  4:220234/147‑1 (MQ=255)                                                                                                                          tCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtgg              >  2:293311/1‑149 (MQ=255)                                                                                                                            gTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtggtg            >  8:284300/1‑149 (MQ=255)                                                                                                                               aTAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCg         >  5:371567/1‑149 (MQ=255)                                                                                                                                   ggCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg      >  7:32953/1‑148 (MQ=255)                                                                                                                                   ggCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg      >  1:129274/1‑148 (MQ=255)                                                                                                                                   atcggCAAAGCGGCCCCCGCGGTTTTCAGCTGGCAGAACTTCCTTCTGCTTCACACCTTCGACACTGATATCGAGGTCCAGGGTCTGCGAACTA‑GCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGa     <  6:300295/145‑1 (MQ=255)                                                                                                                                    gCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTACGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGa     <  1:112670/149‑1 (MQ=255)                                                                                                                                    gCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa    <  2:129274/149‑1 (MQ=255)                                                                                                                                    gCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa    <  3:346840/149‑1 (MQ=255)                                                                                                                                        cAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGa     >  5:300295/1‑144 (MQ=255)                                                                                                                                        cAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGc  >  6:283402/1‑147 (MQ=255)                                                                                                                                                 |                                                                                                                                           CCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCC‑‑TGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTA‑GCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGC  >  NC_002947/5674612‑5674890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑