Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	6,013,885	T→C	intergenic (+90/+20)	xpt → / ← PP_5266	xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,013,885	0	T	C	100.0%	138.7 / NA	43	intergenic (+90/+20)	xpt/PP_5266	xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/23);  total (20/23)

AATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATC  >  NC_002947/6013740‑6014027                                                                                                                                                  |                                                                                                                                               aaTCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGc                                                                                                                                              <  5:289519/148‑1 (MQ=255)              ggTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAAcc                                                                                                                                <  1:283973/149‑1 (MQ=255)                 gAAGTCGCTGGACGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTGCCACAGGGGCAGCACAGGTCTCGAACGCTGTGCCGTACGTGTGGGAGCGGGCGTGCCCGCGAATGGCCGAAACCCtt                                                                                                                             <  7:271058/149‑1 (MQ=38)                  aaGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCttt                                                                                                                            >  7:322756/1‑149 (MQ=255)                      cGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATc                                                                                                                        <  8:229929/149‑1 (MQ=255)                         tGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCCCCCCCCCTCCCACAGGGGCACCACAGGTCTCGCACGCGGTGGCGGACCTGTGGGAGCGGGCGGGCCCCCGAATGGCCGCCACCCGTTTATCTgg                                                                                                                     >  5:354107/1‑149 (MQ=255)                                  gtgtggtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCa                                                                                                            <  2:81270/149‑1 (MQ=255)                                  gtgtggtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCa                                                                                                            <  6:18666/149‑1 (MQ=255)                                     tggtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGcc                                                                                                         <  8:322756/149‑1 (MQ=255)                                            cTTCATCGAATAAACGCGACACCTGCTTCGCGGGCCCCCCCCCACCCCCACGTGCACCACAGGGGTGGGACCCGGGGCCGGACCGGTGGGGGCGGGCGGGGCCCCGAATTGCCGCAACCCCTTTATATGGTTGGCGCCCGCccccccca                                                                                                  >  4:153645/1‑147 (MQ=255)                                                              acacCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAg                                                                                <  4:390597/149‑1 (MQ=255)                                                                     cTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGttctt                                                                           <  1:24692/147‑1 (MQ=255)                                                                        cgcgGGCACGCCCGCTCCCACCGCGCCAGCACCGGTCTCGACCGCTGGGCCTGACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCttt                                                                       <  3:25457/148‑1 (MQ=38)                                                                             gCACCCCCGCTCCCACAGGTGCAGCACAGGTGTCGAACGGTTTGCCGTACCTGTGGTAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGccc                                                                 <  1:148569/149‑1 (MQ=255)                                                                                cGCCCGCTCCCCCTGGTGCAGCACTGGGCTCGACCGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTg                                                              <  4:234680/149‑1 (MQ=39)                                                                                   gcGCTCCCACAGGTGCAGCATAGGTCTCGAACGCTGTGCCGTACCTGTTGGAGCGGGCGTGCCCGCGAATGGCCGCATCCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAAGAAGCGCTGGTAAAGTTCTTCTTTTATCCCCTGCgt                                                           <  8:126418/148‑2 (MQ=38)                                                                                   ccGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCgg                                                           >  6:114917/1‑149 (MQ=255)                                                                                         ccACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGtt                                                                                                                   >  8:229813/1‑87 (MQ=255)                                                                                         ccACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGtt                                                                                                                   <  7:229813/87‑1 (MQ=255)                                                                                         ccACAGGTGCAGCACAGGTCTCGAACGCTGTGCCCTACCTGTGGGAGCGGGCTTGCCCGCGAATTGCCGCAACCCTTTTATCTGGATGCCGCCAGCCACGCCAACAACAAGCGCTGGTAAAGTTATTCTTTAAGcca                                                                 >  8:341822/1‑136 (MQ=255)                                                                                         ccACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCACCCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc                                                     >  3:259478/1‑149 (MQ=21)                                                                                         ccACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc                                                     >  4:41706/1‑149 (MQ=21)                                                                                         ccACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGc                                                       <  5:133880/147‑1 (MQ=21)                                                                                         ccACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGc                                                       >  6:133880/1‑147 (MQ=21)                                                                                           acaGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAg                                                                                <  1:181050/120‑1 (MQ=255)                                                                                           acaGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAg                                                                                >  2:181050/1‑120 (MQ=255)                                                                                           acaGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcccc                                                    >  4:145209/1‑148 (MQ=21)                                                                                              ggTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCACCCCTTTTATCTGGTTGCCGCCAGCCCCGCCACCAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACt                                                >  6:410668/1‑149 (MQ=255)                                                                                                          gTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGccc                                      >  5:113858/1‑147 (MQ=255)                                                                                                               gAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCCTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTgg                               >  5:348627/1‑149 (MQ=255)                                                                                                                       tgCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCa                         <  2:148657/147‑1 (MQ=255)                                                                                                                       tgCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCAt                        <  5:162577/148‑1 (MQ=255)                                                                                                                         ctgcaCCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAc                      <  3:394792/144‑1 (MQ=255)                                                                                                                          cGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAccc                    >  4:140909/1‑149 (MQ=255)                                                                                                                                tgtgGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCgg              >  3:353542/1‑149 (MQ=255)                                                                                                                                   gggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGccc           <  6:354107/149‑1 (MQ=255)                                                                                                                                    ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCa                         >  5:231768/1‑134 (MQ=255)                                                                                                                                    ggAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCa                         <  6:231768/134‑1 (MQ=255)                                                                                                                                     gAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCg         >  7:21293/1‑149 (MQ=255)                                                                                                                                     gAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCg         <  3:153645/149‑1 (MQ=255)                                                                                                                                         gggCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTcc                <  6:163477/138‑1 (MQ=255)                                                                                                                                         gggCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTcc                >  5:163477/1‑138 (MQ=255)                                                                                                                                             gTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATc  >  4:344778/1‑148 (MQ=255)                                                                                                                                                  |                                                                                                                                               AATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATC  >  NC_002947/6013740‑6014027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑