breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 34 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,733,4820CA66.2% 68.4 / 67.0 68R718L (CGC→CTC) sucA2‑oxoglutarate dehydrogenase subunit E1
*NC_0029471,327,1210CT52.5% 20.5 / 155.4 118intergenic (+97/‑196)PP_16SE/PP_23SE16S ribosomal RNA/23S ribosomal RNA
*NC_002947195,6340CT47.2% 30.3 / 135.8 106T380T (ACC→ACTPP_0168putative surface adhesion protein
*NC_002947699,7220AG43.3% 33.8 / 75.7 67intergenic (+115/‑129)PP_t06/PP_23SDtRNA‑Ala/23S ribosomal RNA
*NC_0029475,947,7610CT43.0% 33.2 / 88.5 80R238H (CGC→CAC) rhotranscription termination factor Rho
*NC_0029474,138,4340AG37.8% 51.0 / 74.3 74intergenic (‑249/‑68)PP_3641/PP_3643cytosine/purine/uracil/thiamine/allantoin permease family protein/oxidoreductase
*NC_0029474,348,7050AG35.7% 102.9 / 93.1 98intergenic (‑70/+404)PP_3820/galUgroup II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase
*NC_002947195,5590CA29.6% 128.2 / 65.0 81T355T (ACC→ACAPP_0168putative surface adhesion protein
*NC_0029475,988,9110A.28.1% 199.8 / 81.5 84intergenic (+205/‑341)kefB‑III/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
*NC_002947196,3270AC27.8% 164.4 / 72.1 97T611T (ACA→ACCPP_0168putative surface adhesion protein
*NC_0029475,988,9050TC27.5% 126.1 / 52.7 80intergenic (+199/‑347)kefB‑III/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
*NC_0029474,621,0210A.27.1% 172.8 / 67.3 70coding (31/417 nt)PP_5654phosphotriesterase
*NC_0029475,551,9960TC27.1% 128.5 / 56.4 87S409P (TCA→CCA) PP_4882iron ABC transporter permease
*NC_002947196,3510CG26.7% 181.4 / 69.9 101T619T (ACC→ACGPP_0168putative surface adhesion protein
*NC_0029475,988,8940CT25.9% 146.9 / 48.5 81intergenic (+188/‑358)kefB‑III/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
*NC_0029475,988,8950AG25.9% 136.6 / 49.1 81intergenic (+189/‑357)kefB‑III/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
*NC_0029475,988,8890CA25.6% 165.4 / 41.9 79intergenic (+183/‑363)kefB‑III/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
*NC_002947196,3060TC25.0% 180.3 / 55.7 100N604N (AAT→AACPP_0168putative surface adhesion protein
*NC_0029473,853,1180TA24.2% 51.7 / 15.8 33I169N (ATT→AAT) alkBalpha‑ketoglutarate‑ and Fe(II)‑dependent dioxygenase
*NC_0029475,740,4670TG24.2% 92.0 / 31.9 62intergenic (+32/+39)hutF/PP_5037formiminoglutamate deiminase/lipocalin family lipoprotein

Marginal new junction evidence (lowest skew 10 of 561 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947 4182122 =34 (0.480)29 (0.410) 24/280 3.3 46.0% intergenic (‑137/‑101) PP_3679/PP_3680 hypothetical protein/hypothetical protein
?NC_002947 4625926 = NA (NA)intergenic (+4519/+45) PP_5654/PP_4091 phosphotriesterase/transposase
* ? NC_002947 = 149945829 (0.410)20 (0.290) 20/276 4.1 39.8% intergenic (+35/‑129) trpS/zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
?NC_002947 = 1499495 32 (0.460)intergenic (+72/‑92) trpS/zapE tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
* ? NC_002947 = 65278257 (0.810)20 (0.330) 15/240 4.5 45.0% coding (1961/1962 nt) ctpL methyl‑accepting chemotaxis protein CtpL
?NC_002947 = 4808159 0 (0.000)intergenic (‑268/‑287) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
* ? NC_002947 = 141954932 (0.450)21 (0.300) 17/274 4.8 35.8% intergenic (‑38/+98) PP_1242/PP_1244 hypothetical protein/hypothetical protein
?NC_002947 = 1419636 44 (0.640)intergenic (‑125/+11) PP_1242/PP_1244 hypothetical protein/hypothetical protein
* ? NC_002947 = 418212940 (0.570)21 (0.300) 16/280 5.2 34.4% intergenic (‑144/‑94) PP_3679/PP_3680 hypothetical protein/hypothetical protein
?NC_002947 4536203 = NA (NA)intergenic (+730/‑85) PP_4022/PP_4024 hypothetical protein/transposase
* ? NC_002947 1731163 =44 (0.620)12 (0.210) 11/224 5.4 24.2% intergenic (‑76/+115) dapE/PP_1526 succinyl‑diaminopimelate desuccinylase/beta‑(1‑3)‑glucosyl transferase
?NC_002947 1731255 = 40 (0.710)intergenic (‑168/+23) dapE/PP_1526 succinyl‑diaminopimelate desuccinylase/beta‑(1‑3)‑glucosyl transferase
* ? NC_002947 1967145 =57 (0.810)11 (0.200) 10/214 5.5 22.6% intergenic (+15/+104) PP_1762/yciK hypothetical protein/putative oxidoreductase
?NC_002947 1967241 = 32 (0.590)intergenic (+111/+8) PP_1762/yciK hypothetical protein/putative oxidoreductase
* ? NC_002947 = 378797731 (0.470)15 (0.230)
+CCAACTGT
12/246 5.6 31.5% coding (658/1392 nt) PP_3350 hypothetical protein
?NC_002947 3787978 = 36 (0.510)coding (657/1392 nt) PP_3350 hypothetical protein
* ? NC_002947 = 141954931 (0.440)16 (0.230)
+CT
14/276 5.7 29.9% intergenic (‑38/+98) PP_1242/PP_1244 hypothetical protein/hypothetical protein
?NC_002947 = 1780139 45 (0.640)intergenic (+98/+8) PP_1587/dapC NhaP‑type Na+(K+)/H+ antiporter/N‑succinyl‑L,L‑diaminopimelate aminotransferase
* ? NC_002947 = 4073915NA (NA)14 (0.200) 13/272 5.9 18.6% intergenic (‑453/+171) mdtA/PP_3586 multidrug transporter membrane fusion protein/transposase
?NC_002947 = 4302585 63 (0.890)intergenic (+1075/‑225) PP_3774/PP_5627 hypothetical protein/hypothetical protein