Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,882,212	Δ1 bp	5.1%	coding (1295/1356 nt)	uacT →	uric acid permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,882,212	0	T	.	5.1%	252.1 / 6.9	78	coding (1295/1356 nt)	uacT	uric acid permease
Reads supporting (aligned to +/- strand):  ref base T (42/32);  new base . (2/2);  total (44/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.43e-01

AACCAGCTGTTGGTTGCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACCCGCCGGAATGGGTCGCCCGGG  >  NC_002947/4882064‑4882358                                                                                                                                                       |                                                                                                                                                   aaCCATGTGTTGGTTGCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTGCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCGACTGTCTGTGCCCTGG‑TGTTGAAC‑GTACt                                                                                                                                                    <  7:210124/149‑1 (MQ=255) aaCCAGCTGTTGGTTGCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACt                                                                                                                                                    >  5:72599/1‑149 (MQ=255)     aGCTGTTGGTTGCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTc                                                                                                                                                <  5:165523/149‑1 (MQ=255)     aGCTGTTGGTTGCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTc                                                                                                                                                <  6:72599/149‑1 (MQ=255)        tGTTGGTTGCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCaa                                                                                                                                              >  8:211350/1‑148 (MQ=255)          ttggttgCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCAAGGATGTTGAACAGTAC‑GTTCAACa                                                                                                                                            <  2:395485/149‑1 (MQ=255)              ttgCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCtt                                                                                                                                       >  7:341294/1‑149 (MQ=255)              ttgCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCtt                                                                                                                                       <  6:176394/149‑1 (MQ=255)                gCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTgg                                                                                                                                     >  1:199245/1‑149 (MQ=255)                    tGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGt                                                                                                                                 <  2:314453/149‑1 (MQ=255)                       gCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGGTGAAC‑GTACTGTTCAACATCCTTGGCGGTGcc                                                                                                                              <  6:399588/149‑1 (MQ=255)                         gTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGa                                                                                                                            >  2:8033/1‑149 (MQ=255)                           cGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGAcc                                                                                                                          <  7:276612/149‑1 (MQ=255)                               ttCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACcgcg                                                                                                                       >  3:355544/1‑148 (MQ=255)                                  gggTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAAt                                                                                                                   <  8:162923/149‑1 (MQ=255)                                    gTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATg                                                                                                                  >  2:186623/1‑148 (MQ=255)                                    gTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGA‑TGTTGAACAGTAC‑GTTCAACacca                                                                                                                                         >  6:48382/1‑122 (MQ=255)                                    gTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGA‑TGTTGAACAGTAC‑GTTCAACacca                                                                                                                                         <  5:48382/125‑4 (MQ=255)                                     ttGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGca                                                                                                                >  2:352633/1‑149 (MQ=255)                                         tCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAac                                                                                                            <  7:211350/149‑1 (MQ=255)                                         tCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAac                                                                                                            <  2:199245/149‑1 (MQ=255)                                                 gtgCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCt                                                                                                     <  1:8033/148‑1 (MQ=255)                                                          gAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCa                                                                                           >  5:180217/1‑149 (MQ=255)                                                               cTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAAc                                                                                                                                             >  6:317284/1‑94 (MQ=255)                                                               cTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAAc                                                                                                                                             <  5:317284/94‑1 (MQ=255)                                                               cTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAacgac                                                                                                         <  6:286827/130‑1 (MQ=255)                                                               cTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAacgac                                                                                                         >  5:286827/1‑130 (MQ=255)                                                               cTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAg                                                                                      >  7:44400/1‑149 (MQ=255)                                                                  tGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCAGAACGACGTCTGTCACCAGCATTGAg                                                                                      <  8:44400/146‑1 (MQ=255)                                                                   gCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGcc                                                                                    >  7:322090/1‑147 (MQ=255)                                                                   gCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGcc                                                                                    >  3:241416/1‑147 (MQ=255)                                                                    cccAGATGCCACAGTGGATGGAGCCCTTCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGa                                                                                                                            >  7:153003/1‑106 (MQ=255)                                                                    cccAGATGCCACAGTGGATGGAGCCCTTCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGa                                                                                                                            <  8:153003/106‑1 (MQ=255)                                                                    cccAGATGCCACAGGGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAg                                                                                 <  7:344800/149‑1 (MQ=255)                                                                     ccAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAgg                                                                                >  2:231962/1‑149 (MQ=255)                                                                      cAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAggg                                                                               >  4:147674/1‑149 (MQ=255)                                                                        gATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGTCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGa                                                                                       <  4:221354/139‑1 (MQ=255)                                                                        gATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGTCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGa                                                                                       >  3:221354/1‑139 (MQ=255)                                                                            ccACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCg                                                                          <  6:126367/148‑1 (MQ=255)                                                                             cacaGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGt                                                                        >  8:247048/1‑149 (MQ=255)                                                                              acaGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAgggggg                                                                            >  7:293316/1‑144 (MQ=255)                                                                              acaGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTg                                                                       >  7:381772/1‑149 (MQ=255)                                                                              acaGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTATGTCACCAGCATTGAGCCAAgggggg                                                                            <  8:293316/144‑1 (MQ=255)                                                                                  tggatggaGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATg                                                                   <  3:62495/149‑1 (MQ=255)                                                                                    gatggaGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGc                                                                 >  4:123213/1‑149 (MQ=255)                                                                                    gatggaGCCAATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCACCATTGAGCCAAGGGGGGCGGTGCAGGGc                                                                 >  6:279146/1‑149 (MQ=255)                                                                                           cccATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCt                                                          >  4:88030/1‑149 (MQ=255)                                                                                             cATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCg                                                        >  3:237574/1‑149 (MQ=255)                                                                                             cATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCg                                                        >  6:11145/1‑149 (MQ=255)                                                                                              aTCACCCCAAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACTATGTCTGTAACCAGCATTGAGCCAAGGGCGGCGGTGACTGCCTGCCCCTCGt                                                       >  5:392243/1‑149 (MQ=255)                                                                                              aTCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGt                                                       <  1:352633/149‑1 (MQ=255)                                                                                               tCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGtt                                                      <  4:355544/149‑1 (MQ=255)                                                                                                  gtcactGCGGTATGGCCTGGGCCACGGTCTGTGCCCTGG‑TGTTGATC‑GTACTGTTCAACTTCCTTGGCGGTGCCGAGCGCGCAATGCACAACGTCGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCa                                                    <  6:140668/146‑1 (MQ=255)                                                                                                    cacaGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGTGGCGGTGCATGGCTGCCCCTCGTTCAcc                                                  >  5:386767/1‑148 (MQ=255)                                                                                                     acaGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACctc                                                >  1:361066/1‑149 (MQ=255)                                                                                                     acaGCGGTATCGCCATCGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTCTACAACATCCTTGGCGGGGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGGTCAca                                                  >  5:140668/1‑146 (MQ=255)                                                                                                      caGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGc                                                                           <  2:407680/121‑1 (MQ=255)                                                                                                      caGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGc                                                                           >  1:407680/1‑121 (MQ=255)                                                                                                        gCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGTATGGCTGCCCCTCGTTCACCTCTGc                                             <  8:322090/149‑1 (MQ=255)                                                                                                         cGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCa                                            <  3:147674/149‑1 (MQ=255)                                                                                                           gTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAg                                           >  3:5713/1‑148 (MQ=255)                                                                                                                 ccATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGc                                    >  3:350574/1‑149 (MQ=255)                                                                                                                 ccATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCATCCTTCACCTGTGCAGTAAccc                                     >  8:252203/1‑147 (MQ=255)                                                                                                                   aTGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAgggg                                                                              >  2:49551/1‑105 (MQ=255)                                                                                                                   aTGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTa                                  <  1:231962/149‑1 (MQ=255)                                                                                                                   aTGGCCACGGTCTGTGCCCTGG‑TGTTCAAC‑GTACTGTTCAACATCCTTGGCGGTGCGTACCGCGAAATGCAATACGACGTCTGTCACCAGCATTGAGCCAAgggg                                                                              <  1:49551/105‑1 (MQ=255)                                                                                                                        cACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAAc                                       <  7:26/139‑1 (MQ=255)                                                                                                                        cACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAAc                                       >  8:26/1‑139 (MQ=255)                                                                                                                            gTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGa                         <  8:341294/149‑1 (MQ=255)                                                                                                                                 tgCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACccgc                    >  7:44498/1‑149 (MQ=255)                                                                                                                                   cccTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACccgccg                  >  7:372341/1‑149 (MQ=255)                                                                                                                                   cccTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACccgccg                  >  5:172755/1‑149 (MQ=255)                                                                                                                                    ccTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACccgccg                  >  8:209774/1‑148 (MQ=255)                                                                                                                                      tGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACCCGCCGGaa               >  1:19362/1‑149 (MQ=255)                                                                                                                                          tgtTGAACAGTAC‑GTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGCCCCGCCGGAATgg            >  7:345857/1‑149 (MQ=255)                                                                                                                                              gAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACCCGCCGGAATGGGTc         <  3:123213/148‑1 (MQ=255)                                                                                                                                               aaC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCa                                            >  7:38530/1‑112 (MQ=255)                                                                                                                                               aaC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCa                                            <  8:38530/112‑1 (MQ=255)                                                                                                                                                   gTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACCCGCCGGAATGGGTCGCCCg    >  3:397312/1‑149 (MQ=255)                                                                                                                                                      cTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACCCGCCGGAATGGGTCGCCCggg  <  4:350574/148‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                   AACCAGCTGTTGGTTGCCGTGAGCGTCGGCTTCGGGTTGATCCCCGTGGTGCGTCCGGAGTTCTTTGCCCAGATGCCACAGTGGATGGAGCCCATCACCCACAGCGGTATCGCCATGGCCACGGTCTGTGCCCTGG‑TGTTGAAC‑GTACTGTTCAACATCCTTGGCGGTGCCGACCGCGCAATGCACAACGACGTCTGTCACCAGCATTGAGCCAAGGGGGGCGGTGCATGGCTGCCCCTCGTTCACCTCTGCAGTAACCGCTACGCCGTCGACCCGCCGGAATGGGTCGCCCGGG  >  NC_002947/4882064‑4882358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑