Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,124,166 Δ1 bp 8.5% intergenic (‑35/‑93) PP_4510 ← / → PP_4511 hypothetical protein/AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,124,1660A. 8.5% 146.5 / 6.8 47intergenic (‑35/‑93)PP_4510/PP_4511hypothetical protein/AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (21/22);  new base . (2/2);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CTGCGACAGGTGTTCGGCCTGGCAGTCGCGGGTACCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATGGGCGCCTGCCCTGCGAGGAAGCCCCCATGACCGCCGGCCCGATCCTCTCGCTGCGCCATTACCGCGACAGCCTGATCGCC  >  NC_002947/5124021‑5124312
                                                                                                                                                 |                                                                                                                                                  
cTGCGACAGGTGTTCGGCCTGGCAGTCGCGGGTACCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTggcaggc                                                                                                                                                 <  8:113086/149‑1 (MQ=255)
 tGCGACAGGTGTTCGGCCTGGCAGTGGCGGGTACCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTggcaggca                                                                                                                                                <  2:327022/149‑1 (MQ=255)
 tGCGACAGGTGTTCGGCCTGGCAGTCGCGGGTACCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTggcaggca                                                                                                                                                >  2:123437/1‑149 (MQ=255)
  gCGACAGGTGTTCGGCCTGGCAGTCGCGGGTACCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTggcaggca                                                                                                                                                >  4:391245/1‑148 (MQ=255)
   cGACAGGTGTTCGGCCTGGCAGTCGCGGGTACCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGcac                                                                                                                                               <  4:361690/148‑1 (MQ=255)
    gACAGGTGTTCGGCCTGGCAGTCGCGGGTACCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGcacac                                                                                                                                             <  2:129027/149‑1 (MQ=255)
            ttCGGCCTGGCAGTCGCGGGTACCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGcacac                                                                                                                                             <  8:299986/141‑1 (MQ=255)
            ttCGGCCTGGCAGTCGCGGGTACCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGcacac                                                                                                                                             >  7:299986/1‑141 (MQ=255)
            ttCGGCCTGGCAGTCGCGGGTACCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAgc                                                                                                                                     >  5:29948/1‑149 (MQ=255)
             tCGGCCTGGCAGTCGCGGGTACCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAgcg                                                                                                                                    >  5:317611/1‑149 (MQ=255)
                gCCTGGCAGTCGCGGGTGCCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCa                                                                                                                                 >  5:67916/1‑149 (MQ=255)
                 ccTGGCAGTCGCGGGTACCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCAt                                                                                                                                <  5:31377/149‑1 (MQ=255)
                  cTGGCAGTCGCGGGTACCCAGGTGCACGTCGGAGATCCACTGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATg                                                                                                                               <  3:136621/149‑1 (MQ=255)
                  cTGGCAGTCGCGGGTACCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATg                                                                                                                               <  2:227674/149‑1 (MQ=255)
                   tGGCAGTCGCGGGTACCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTggcagg                                                                                                                                                  >  3:299591/1‑129 (MQ=255)
                     gCAGTCGCGGGTACCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGc                                                                                                                             >  3:74596/1‑148 (MQ=255)
                       aGTCGCGGGTACCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCtaa                                                                                                                          <  6:317611/149‑1 (MQ=255)
                           gcgGGTACCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGCCAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGC‑GGCACACATGACAGCGCCATGGCAAAACGGt                                                                                                                     <  7:282544/149‑1 (MQ=255)
                           gcgGGTACCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGC‑GGCACACATGACAGCGCCATGGCAAAACGGt                                                                                                                     >  8:282544/1‑149 (MQ=255)
                           gcgGGTACCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGC‑GGCACACATGACAGCGCCATGGCAAAACGGt                                                                                                                     <  7:287994/149‑1 (MQ=255)
                           gcgGGTACCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGC‑GGCACACATGACAGCGCCATGGCAAAACGGt                                                                                                                     >  8:287994/1‑149 (MQ=255)
                            cgGGTACCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGt                                                                                                                     <  4:74596/149‑1 (MQ=255)
                                   ccAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGt                                                                                                              >  5:26731/1‑149 (MQ=255)
                                           aCGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCGGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGt                                                                                                      >  2:273872/1‑149 (MQ=255)
                                             gTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTg                                                                                                     >  4:320561/1‑148 (MQ=255)
                                              tCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCa                                                                                                   <  6:165926/149‑1 (MQ=255)
                                                  agaTCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGa                                                                                               <  6:67916/149‑1 (MQ=255)
                                                   gaTCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGAt                                                                                              <  1:123437/149‑1 (MQ=255)
                                                      ccACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGTGACAGTTCGGCATCGCTCATGGGCACGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATCACAGCGCCAAGGCAAAACGCTCACGAGAGATGGCGTGCAGCGAtt                                                                                             >  6:57864/1‑147 (MQ=255)
                                                      ccACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGAtt                                                                                             >  4:139540/1‑147 (MQ=255)
                                                                 gcacgcGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATggg                                                                                <  6:29948/149‑1 (MQ=255)
                                                                  cacgcGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATGGgc                                                                               <  5:337238/149‑1 (MQ=255)
                                                                  cacgcGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATGGgc                                                                               >  3:267381/1‑149 (MQ=255)
                                                                   acgcGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATGGgcg                                                                              >  4:397674/1‑149 (MQ=255)
                                                                              gcgcGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATGGGCGcctgccctgc                                                                    <  3:397674/148‑1 (MQ=255)
                                                                               cgcgAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATGGGCGCCTGCCCTGCGa                                                                  <  3:320561/149‑1 (MQ=255)
                                                                                gcgAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATGGGCGCCTGCCCTGCGAg                                                                 <  8:31504/149‑1 (MQ=255)
                                                                                   aGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATGGGCGCCTGCCCTGCGAGGaa                                                              <  6:26731/149‑1 (MQ=255)
                                                                                       gCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATGGGCGCCTGCCCTGCGAGGAAGccc                                                          >  3:75943/1‑149 (MQ=255)
                                                                                            aCAGTTCGGCATGTCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATTGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATGGGCGCCTGCCCTGCGAGGAAGCCCCCATg                                                     <  4:267381/149‑1 (MQ=255)
                                                                                                    gCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCGCAATGGGCGCCTGCCCTGCGAGGAAGCCCCCATGACCGCCgg                                             >  3:352183/1‑149 (MQ=255)
                                                                                                    gCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATGGGCGCCTGCCCTGCGAGGAAGCCCCCATGACCGCCgg                                             >  8:63445/1‑149 (MQ=255)
                                                                                                       tGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATGGGCGCCTGCCCTGCGAGGAAGCCCCCATGACCGCCGGccc                                          <  3:139540/149‑1 (MQ=255)
                                                                                                           tCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATGGGCGCCTGCCCTGCGAGGAAGCCCCCATGACCGCCGGCCCGATc                                      >  8:196628/1‑149 (MQ=255)
                                                                                                             aTGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGTGATTGAGGCACAATGGGCGCCTGCCCTGCGAGGAAGCCCCCATGACCGCCGGCCCGATc                                      <  7:63445/147‑1 (MQ=255)
                                                                                                             aTGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATGGGCGCCTGCCCTGCGAGGAAGCCCCCATGACCGCCGGCCCGATc                                      <  3:391245/147‑1 (MQ=255)
                                                                                                                ggCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATGGGCGCCTGCCCTGCGAGGAAGCCCCCATGACCGCCGGCCCGATCCtctc                                 >  6:318587/1‑149 (MQ=255)
                                                                                                                       tCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATGGGCGCCTGCCCTGCGAGGAAGCCCCCATGACCGCCGGCCCGATCCTCTCGCTgcgc                          >  4:377123/1‑149 (MQ=255)
                                                                                                                                gTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATGGGCGCCTGCCCTGCGAGGAAGCCCCCATGACCGCCGGCCCGATCCTCTCGCTGCGCCATTACcgc                 >  2:391672/1‑149 (MQ=255)
                                                                                                                                           aCTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATGGGCGCCTGCCCTGCGAGGAAGCCCCCATGACCGCCGGCCCGATCCTCTCGCTGCGCCATTACCGCGACAGCCTGAt      >  5:368525/1‑149 (MQ=255)
                                                                                                                                            cTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATGGGCGCCTGCCCTGCGAGGAAGCCCCCATGACCGCCGGCCCGATCCTCTCGCTGCGCCATTACCGCGACAGCCTGATc     <  1:273872/149‑1 (MQ=255)
                                                                                                                                             tGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATGGGCGCCTGCCCTGCGAGGAAGCCCCCATGACCGCCGGCCCGATCCTCTCGCTGCGCCATTACCGCGACAGCCTGATCg    >  8:99874/1‑149 (MQ=255)
                                                                                                                                                caggcaCACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATGGGCGCCTGCACTGCGAGGAAGCCCCCATGACCGCCGGCCCGATCCTCTCGCTGCGCCATTACCGCGACAGCCTGATCGcc  >  7:166375/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CTGCGACAGGTGTTCGGCCTGGCAGTCGCGGGTACCCAGGTGCACGTCGGAGATCCACAGGGTACGCACGCGTTGCTTGCGCGAGGGGCGGGACAGTTCGGCATGGCTCATGGGCAGGCTCCGGCGCAGTTTGCTGGAGACTGGCAGGCACACATGACAGCGCCATGGCAAAACGGTGACGAGTGATGGCGTGCAGGGATTGAGGCACAATGGGCGCCTGCCCTGCGAGGAAGCCCCCATGACCGCCGGCCCGATCCTCTCGCTGCGCCATTACCGCGACAGCCTGATCGCC  >  NC_002947/5124021‑5124312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑