| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,344,490 | Δ1 bp | 14.3% | intergenic (+65/‑113) | pgi‑II → / → acsA‑II | glucose 6‑phosphate isomerase/acetyl‑CoA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,344,490 | 0 | A | . | 14.3% | 77.8 / 8.3 | 28 | intergenic (+65/‑113) | pgi‑II/acsA‑II | glucose 6‑phosphate isomerase/acetyl‑CoA synthetase |
| Reads supporting (aligned to +/- strand): ref base A (11/13); new base . (2/2); total (13/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
GGTAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑CCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGA‑‑ACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGC > NC_002947/5344348‑5344631 | ggTAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑CCTACCAGGCAACGCGTTACCTCGTAGGAGCggg < 1:160462/148‑1 (MQ=255)ggTAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑CCTACCAGGCAACGCGTTACCTCGTAGGAGCggg < 3:137126/148‑1 (MQ=255) gCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCATCTAGTTCCGCGGCCGGCACCGCGGTTGATCCGTGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑CCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTa < 7:184025/148‑1 (MQ=255) cTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGAGGTAACGCGTTGCCTGGTAG‑‑GCGGGTTTAccc < 4:162608/149‑1 (MQ=21) aCAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑CCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAaga < 1:372604/149‑1 (MQ=255) cAGCGCCGAAGACGGTTCCACCCAAGGCCTGAGCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑CCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAagag < 5:98562/149‑1 (MQ=255) aGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑CCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAgg < 2:289584/149‑1 (MQ=255) ggTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑CCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGtt > 1:305708/1‑149 (MQ=255) gATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑CCTACCAGGCAACGCGTTACCTCGTAGGAGc < 3:355359/101‑1 (MQ=255) gATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑CCTACCAGGCAACGCGTTACCTCGTAGGAGc > 4:355359/1‑101 (MQ=255) ttctTCCGCGCCCGTCCCGGGGGTTGATCCGCGCTTGGGCCGGCGTATTCGCGGGTAAACCCG‑‑CCTACCAGGAAACGGGTTACCTCTTTGGAGGGGTTTTACCCGCTAAGTGGCCGGCCCAGGTTGA‑‑ACACCGCTCCCAGCTACACTGt < 2:305708/147‑1 (MQ=255) aggcgGGTTTACCCG‑‑CCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGA‑‑AGACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGacc > 7:262069/3‑149 (MQ=255) gTAAACCCG‑‑CCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGA‑‑ACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGc < 8:219752/124‑1 (MQ=255) gTAAACCCG‑‑CCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGA‑‑ACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGc > 4:370658/1‑149 (MQ=255) aaaCCCG‑‑CCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGA‑‑ACACCGCTCCCAGCTACACTGt > 5:173331/1‑93 (MQ=255) aaaCCCG‑‑CCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGA‑‑ACACCGCTCCCAGCTACACTGt < 6:173331/93‑1 (MQ=255) acccgctccTACGAGGTAACGCGTTGCCTGGTAG‑‑GCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGA‑‑ACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATg > 8:309607/8‑149 (MQ=25) acccgctccTACGAGGTAACGCGTTGCCTGGTAG‑‑GCGGGTTTACCCGCGAAGAGGCCGGCACAGGTTGA‑‑ACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATg > 4:177263/8‑149 (MQ=21) aCCCG‑‑CCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGA‑‑ACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATg > 7:192292/1‑149 (MQ=255) ccG‑‑CCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAAGACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCTTTGAAGTCCATCGCCCCTTACAAGAGAAGGACCACCCGCCATg > 1:120249/1‑149 (MQ=38) ccG‑‑CCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGA‑‑ACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGtt > 5:27811/1‑149 (MQ=255) gctccTACGAGGTAACGCGTTGCCTGGTAG‑‑GCGGGTTTACCCGCGAAGAGGCCGGCACAGGTTGA‑‑ACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCg < 3:177263/146‑1 (MQ=25) cAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGa‑‑a < 4:89916/56‑1 (MQ=255) cAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGa‑‑a > 3:89916/1‑56 (MQ=255) ccTCGTTGTAGGGGTTTTACCGGTGAAGAGGCCGGCCCAGGTTGA‑‑ACACCGCTCCCAGCTACACTTTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAg < 5:301179/149‑1 (MQ=255) cTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGA‑‑ACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCa < 6:330168/109‑1 (MQ=255) cTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGA‑‑ACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCa > 5:330168/1‑109 (MQ=255) tCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGA‑‑ACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGc > 8:87369/1‑149 (MQ=255) | GGTAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCG‑‑CCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGA‑‑ACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGC > NC_002947/5344348‑5344631 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |