Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,925,584	Δ1 bp	9.3%	coding (561/1083 nt)	gcvT‑II ←	aminomethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,925,584	0	G	.	9.3%	269.2 / 20.8	86	coding (561/1083 nt)	gcvT‑II	aminomethyltransferase
Reads supporting (aligned to +/- strand):  ref base G (37/40);  new base . (3/5);  total (41/45)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAGATCAGG  >  NC_002947/5925437‑5925730                                                                                                                                                      |                                                                                                                                                   gCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGc                                                                                                                                                   <  4:395872/149‑1 (MQ=255)  ccGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGcc                                                                                                                                                  <  8:40439/149‑1 (MQ=255)        aGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGGCTATA‑CCGGTgag                                                                                                                                            <  5:137984/149‑3 (MQ=255)        aGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCg                                                                                                                                            <  4:125846/149‑1 (MQ=255)         ggTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCgg                                                                                                                                           <  2:270230/149‑1 (MQ=255)          gTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCggg                                                                                                                                          >  5:298909/1‑149 (MQ=255)          gTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCggg                                                                                                                                          <  5:319323/149‑1 (MQ=255)          gTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCggg                                                                                                                                          <  1:25578/149‑1 (MQ=255)          gTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACAAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCggg                                                                                                                                          >  6:3216/1‑149 (MQ=255)            tCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGGCTATA‑CCGGTGAggacg                                                                                                                                        >  8:14562/1‑146 (MQ=255)            tCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGGCTATA‑CCGGTGAggacg                                                                                                                                        >  8:20449/1‑146 (MQ=255)             cATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGGCTATA‑CCGGTGAggacg                                                                                                                                        <  7:20449/148‑4 (MQ=255)             cATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGGCTATA‑CCGGTGAggacg                                                                                                                                        <  7:14562/148‑4 (MQ=255)               tGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAAt                                                                                                                                      >  7:92677/1‑148 (MQ=255)                 ccGGCTTCCAGGCGCAGGGTATCGCTGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGGCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGa                                                                                                                                    <  3:326084/148‑1 (MQ=255)                 ccGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGaa                                                                                                                                   >  6:265008/1‑149 (MQ=255)                 ccGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGaa                                                                                                                                   <  6:271400/149‑1 (MQ=255)                 ccGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCGCACCGGCTATA‑CCGGTGAggacggcct                                                                                                                                    <  6:303472/148‑8 (MQ=255)                 ccGGCTTCCAGGCACAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGaa                                                                                                                                   <  7:64748/149‑1 (MQ=255)                    gCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAAc                                                                                                                                  >  1:402054/1‑147 (MQ=255)                      ttCCAGGCGCAGGGAATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATATC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCACTGAACAAGt                                                                                                                              >  7:346743/1‑149 (MQ=255)                            gcgcAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGGCTATA‑CCGGTga                                                                                                                                             <  1:387287/128‑2 (MQ=255)                            gcgcAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGGCTATA‑CCGGTga                                                                                                                                             >  2:387287/1‑127 (MQ=255)                            gcgcAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGt                                                                                                                        <  6:35780/149‑1 (MQ=255)                              gcAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCTCCGACCAGGTCGTTGAAGAAGGCCACCTCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGTCAATGAACCAGTCGCCGTCg                                                                                                                      <  4:132315/149‑1 (MQ=255)                                aGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGc                                                                                                                    <  5:183449/149‑1 (MQ=255)                                        gcgGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCTGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGccc                                                                                                             <  4:160827/148‑1 (MQ=255)                                        gcgGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCt                                                                                                            >  6:188507/1‑149 (MQ=255)                                             cgcCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAAc                                                                                                       <  5:265008/149‑1 (MQ=255)                                              gcCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCATCCTCACCGG‑TATAGCCGGTGCGGGCAATGaa                                                                                                                                   <  3:300052/120‑1 (MQ=255)                                              gcCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCATCCTCACCGG‑TATAGCCGGTGCGGGCAATGaa                                                                                                                                   >  4:300052/1‑120 (MQ=255)                                               cccAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACgg                                                                                                     >  3:116625/1‑149 (MQ=255)                                                    gCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCa                                                                                                <  6:24260/149‑1 (MQ=255)                                                    gCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCa                                                                                                <  5:188507/149‑1 (MQ=255)                                                    gCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCAGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGgcg                                                                                                  >  2:312432/1‑147 (MQ=255)                                                            gggCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTcgcg                                                                                         <  4:185441/148‑1 (MQ=255)                                                             ggCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCg                                                                                                                            >  8:167731/1‑112 (MQ=255)                                                             ggCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCg                                                                                                                            <  7:167731/112‑1 (MQ=255)                                                             ggCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCggc                                                                                       <  8:363292/149‑2 (MQ=255)                                                             ggCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGa                                                                                       <  6:298909/149‑1 (MQ=255)                                                              gCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCGGAGGCCGTCATAACCGG‑TTAACCGGGTGCGGGCAATGAACAAGTCGCCGACGGAAACCCCCACCAACGGGCGCCGTTCCCGGa                                                                                       >  1:325526/1‑149 (MQ=255)                                                              gCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCACCGCCCTCGAACGGGCGCAGTTCGCGGAt                                                                                      >  8:230392/1‑149 (MQ=255)                                                              gCGATACCGGCGCCGACAAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGAt                                                                                      <  5:3216/149‑1 (MQ=255)                                                               cGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGGCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGAt                                                                                      >  1:25979/1‑148 (MQ=255)                                                                gATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGa                                                                                    <  2:283029/149‑1 (MQ=255)                                                                     cGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAg                                                                                                                               >  7:41882/1‑101 (MQ=255)                                                                     cGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAg                                                                                                                               <  8:41882/101‑1 (MQ=255)                                                                            gACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAgcgcgcg                                                                        >  3:333769/1‑149 (MQ=255)                                                                                      ttGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAg                                                              >  5:276877/1‑149 (MQ=255)                                                                                        gaagaaGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAg                                                              >  3:153671/1‑147 (MQ=255)                                                                                        gaagaaGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAg                                                              >  3:202206/1‑147 (MQ=255)                                                                                          agaagGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAgg                                                          <  2:25979/149‑1 (MQ=255)                                                                                            aagGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGGCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGgc                                                        <  7:230392/149‑1 (MQ=255)                                                                                                ccACCGCCTGATCGCCCTGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGgccgcc                                                    <  1:288979/149‑1 (MQ=255)                                                                                                 cACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCa                                                   >  2:312430/1‑149 (MQ=255)                                                                                                  aCCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCAc                                                  >  3:200609/1‑149 (MQ=255)                                                                                                  aCCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCAAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCAc                                                  >  6:117817/1‑149 (MQ=255)                                                                                                         gATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTcg                                           >  7:300192/1‑149 (MQ=255)                                                                                                          aTCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTcgc                                          <  2:14150/149‑1 (MQ=255)                                                                                                          aTCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTcgc                                          <  4:153671/149‑1 (MQ=255)                                                                                                           tCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTcgcg                                         <  1:239114/149‑1 (MQ=255)                                                                                                             gCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCgg                                        <  1:312432/148‑1 (MQ=255)                                                                                                              cccGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCggg                                       >  1:114836/1‑148 (MQ=255)                                                                                                              cccGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCGGAGCAGGGCCGCCACCTTTTCGCGGGc                                      >  7:294284/1‑149 (MQ=255)                                                                                                                  ggAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGACCCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCAcgc                                  <  7:138065/149‑1 (MQ=255)                                                                                                                     aGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGgccgcc                                                    <  7:156423/128‑1 (MQ=255)                                                                                                                     aGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGgccgcc                                                    >  8:156423/1‑128 (MQ=255)                                                                                                                      gatgatCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCCCGCGGcc                              >  8:330884/1‑149 (MQ=255)                                                                                                                         gatCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCt                           <  8:294284/149‑1 (MQ=255)                                                                                                                             tc‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCttt                                              >  7:21133/1‑126 (MQ=255)                                                                                                                             tc‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCttt                                              <  8:21133/126‑1 (MQ=255)                                                                                                                              c‑cAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATg                      >  4:54910/1‑149 (MQ=255)                                                                                                                              c‑cAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATg                      >  1:363210/1‑149 (MQ=255)                                                                                                                                cAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGAt                       <  4:202206/147‑1 (MQ=255)                                                                                                                                cAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATg                      <  2:402054/148‑1 (MQ=255)                                                                                                                                cAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATg                      <  4:333769/148‑1 (MQ=255)                                                                                                                                  ggCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGc                    >  2:77776/1‑148 (MQ=255)                                                                                                                                     cGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAg                 >  1:313552/1‑148 (MQ=255)                                                                                                                                          tCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACACCATCGAAAGGACGAAGTTCGGGGATGAGTGCAGCGCGCGCGGGGATGAGCAGGGCCGCAACCTTTTCGCGGGCACGCGGCCCCAGGATCGCAAGGATGGc           >  8:255966/1‑149 (MQ=255)                                                                                                                                           cACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGc           >  3:340438/1‑148 (MQ=255)                                                                                                                                           cACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGc           >  5:59465/1‑148 (MQ=255)                                                                                                                                            aCCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGc           >  8:305138/1‑147 (MQ=255)                                                                                                                                             ccGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAg        <  7:305138/149‑1 (MQ=255)                                                                                                                                                g‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAGAt      >  3:251042/1‑148 (MQ=255)                                                                                                                                                  tataGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAGATCa    <  5:117817/149‑1 (MQ=255)                                                                                                                                                    taGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAGATCAgg  >  2:356827/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                   GCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAGATCAGG  >  NC_002947/5925437‑5925730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑