Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,023,484	Δ1 bp	5.6%	coding (516/897 nt)	rmd →	oxidoreductase Rmd

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,023,484	0	G	.	5.6%	230.9 / 6.5	71	coding (516/897 nt)	rmd	oxidoreductase Rmd
Reads supporting (aligned to +/- strand):  ref base G (36/31);  new base . (2/2);  total (38/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGGGATGCTGGGCGAGCAGACCCCGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAGGCACGCCCGC  >  NC_002947/2023339‑2023632                                                                                                                                                  |                                                                                                                                                      aaGGGATGCTGGGCGAGCAGACCCCGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATTCATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGggcagg                                                                                                                                                      >  7:361226/1‑147 (MQ=255)    ggATGCTGGGCGAGCAGACCCCGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGggcaggca                                                                                                                                                    >  7:281017/1‑146 (MQ=255)           gggCGAGCAGACCCCGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCCTCACGCGGCCGTTCAATTACACCGGTGTTGGGCAGGCAGAGAAtttac                                                                                                                                          >  5:167145/1‑147 (MQ=255)           gggCGAGCAGACCCCGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTcc                                                                                                                                          >  8:188992/1‑149 (MQ=255)                    gACCCCGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGccc                                                                                                                                 >  1:42623/1‑148 (MQ=255)                     aCCCCGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCA‑GCAGGAAATTCTCTGCCTGCCCaa                                                                                                                               <  5:187428/149‑1 (MQ=255)                       cccGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAAttt                                                                                                                                            >  8:178356/1‑135 (MQ=255)                       cccGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAAttt                                                                                                                                            <  7:178356/135‑1 (MQ=255)                         cGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGAtt                                                                                                                           >  1:217339/1‑149 (MQ=255)                               cgcCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGGCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTc                                                                                                                      <  8:80640/148‑1 (MQ=255)                                  cGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCAt                                                                                                                  >  1:367513/1‑149 (MQ=255)                                    gCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCAttt                                                                                                                >  4:189993/1‑149 (MQ=255)                                      cAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTc                                                                                                              >  7:272432/1‑149 (MQ=255)                                          gATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTg                                                                                                                          <  1:17893/133‑1 (MQ=255)                                          gATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTg                                                                                                                          >  2:17893/1‑133 (MQ=255)                                           aTTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTcgccgc                                                                                                         <  2:42623/149‑1 (MQ=255)                                             tACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTcgccgc                                                                                                         >  3:159405/1‑147 (MQ=255)                                              aCGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCaa                                                                                                       <  2:367513/148‑1 (MQ=255)                                                gcgGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGc                                                                                                    >  7:286890/1‑149 (MQ=255)                                                  ggTCAGCAATTTGTCCTTGTAATACCTGGCGCGGCTTTTGTGGTACCGTTTTCCTTTCGTCTTCACGCTTCCTTTCTATTACACCGGTGTTGGGCAGGCAGAGATTTTCCTG‑CGGCGCAAGATTGTTTCGCATTTTCTCCTAAAGGCCg                                                                                                  <  6:167145/149‑1 (MQ=255)                                                    tCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGa                                                                                                 >  7:83423/1‑148 (MQ=255)                                                       gCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACAcc                                                                                             <  4:350571/149‑1 (MQ=255)                                                        cAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGcc                                                                                                   <  4:160070/142‑1 (MQ=255)                                                        cAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGcc                                                                                                   >  3:160070/1‑142 (MQ=255)                                                        cAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGaca                                                                                               >  1:251603/1‑146 (MQ=255)                                                        cAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGaca                                                                                               <  2:251603/146‑1 (MQ=255)                                                           gTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCg                                                                                         <  8:281017/149‑1 (MQ=255)                                                            ttGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGa                                                                                        <  4:159405/149‑1 (MQ=255)                                                            cgcgCCATGTATTCCATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATc                                                                                          >  3:349806/4‑147 (MQ=255)                                                              ggCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAg                                                                                       <  4:75132/148‑1 (MQ=255)                                                                ccATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTc                                                                                    >  3:106821/1‑149 (MQ=255)                                                                 cATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATc                                                                                          >  7:339850/1‑142 (MQ=255)                                                                      attccATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCg                                                                                   >  3:245852/5‑144 (MQ=255)                                                                      attccATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCg                                                                                   <  4:245852/140‑1 (MQ=255)                                                                      aaTACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAAc                                                                              <  8:50105/149‑1 (MQ=255)                                                                          cATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTa                                                                           <  1:190398/148‑1 (MQ=255)                                                                           aTGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCCCGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGAAAGGCCGACACCATCGAGCTCGGCAACCTAGa                                                                         >  6:287532/1‑149 (MQ=255)                                                                           aTGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGa                                                                         >  5:169480/1‑149 (MQ=255)                                                                           aTGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCCCAAGGCCGACACCATCGAGCTCGGCAACCTAGa                                                                         >  7:352459/1‑149 (MQ=255)                                                                            tGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGAc                                                                        >  2:305931/1‑149 (MQ=255)                                                                            tGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGAc                                                                        <  8:272432/149‑1 (MQ=255)                                                                             ggCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGAc                                                                        <  4:349806/148‑1 (MQ=255)                                                                              gcgcGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACg                                                                       <  6:169480/148‑1 (MQ=255)                                                                               cgcgGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTa                                                                     <  2:217339/149‑1 (MQ=255)                                                                               cgcgGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTATCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTa                                                                     <  5:121661/149‑1 (MQ=255)                                                                                gcgGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTAt                                                                    <  5:241384/149‑1 (MQ=255)                                                                                         gtgtGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATc                                                                                          >  6:20776/1‑118 (MQ=255)                                                                                         gtgtGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATc                                                                                          <  5:20776/118‑1 (MQ=255)                                                                                           gtgACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGAttt                                                          >  5:363627/1‑148 (MQ=255)                                                                                                 gTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGAt                                                   <  7:188992/149‑1 (MQ=255)                                                                                                  tttGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGAAACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATg                                                  >  7:55860/1‑149 (MQ=255)                                                                                                          tCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTg                                            <  8:361226/147‑1 (MQ=255)                                                                                                          tCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGc                                           <  8:83423/148‑1 (MQ=255)                                                                                                          tCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGc                                           <  6:363627/148‑1 (MQ=255)                                                                                                          tCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCg                                          >  6:44416/1‑149 (MQ=255)                                                                                                               atcaCGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCTACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGt                                     <  7:259844/149‑1 (MQ=255)                                                                                                               atcaCGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCg                                      <  1:305931/148‑1 (MQ=255)                                                                                                                   cgcgTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAg                                 >  4:76978/1‑149 (MQ=255)                                                                                                                    gcgTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAgg                                >  4:231662/1‑149 (MQ=255)                                                                                                                      gTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCg                              >  8:147535/1‑149 (MQ=255)                                                                                                                      gTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGAGATTTCAGCGATGTACGTGCGGTCGTGCAGGCg                              >  4:384613/1‑149 (MQ=255)                                                                                                                               aTTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGc                     <  3:189993/149‑1 (MQ=255)                                                                                                                                    acCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGAt                >  3:116659/1‑149 (MQ=255)                                                                                                                                      cGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTAc                                               <  3:350816/116‑1 (MQ=255)                                                                                                                                      cGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTAGGGCGCGATTTCAGCGATGTAc                                               >  4:350816/1‑116 (MQ=255)                                                                                                                                       ggTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATc               >  7:97375/1‑147 (MQ=255)                                                                                                                                        gtgtTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGaacccg            >  8:358692/1‑144 (MQ=255)                                                                                                                                        gtgtTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAg            <  3:231662/149‑1 (MQ=255)                                                                                                                                           ttGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGGCGTGCAGGCGTACCGCGGCTTGATCGAGGCa         >  3:212220/1‑149 (MQ=255)                                                                                                                                           ttGGGCA‑GCAGGAAATTCTCTGCCTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACcg                         >  3:128469/1‑133 (MQ=255)                                                                                                                                           ttGGGCA‑GCAGGAAATTCTCTGCCTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACcg                         <  4:128469/133‑1 (MQ=255)                                                                                                                                           ttGGGCA‑GCAGGAAATTCTCTGCCTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAGGCa         >  3:304659/1‑149 (MQ=255)                                                                                                                                             gggCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAGGCACg       >  8:205274/1‑149 (MQ=255)                                                                                                                                                  ggcagAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAGGCACGCCCGc  <  8:97375/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                      AAGGGATGCTGGGCGAGCAGACCCCGCCAGCGCCGGCCAATGATTACGCGGTCAGCAAGTTGGCCATGGAATACATGGCGCGGCTGTGGTGTGACCGTTTGCCTATCGTCATCACGCGTCCTTTCAATTACACCGGTGTTGGGCAGGCAGAGAATTTCCTG‑CTGCCCAAGATTGTTTCGCATTTTCGCCGCAAGGCCGACACCATCGAGCTCGGCAACCTAGACGTATGGCGCGATTTCAGCGATGTACGTGCGGTCGTGCAGGCGTACCGCGGCTTGATCGAGGCACGCCCGC  >  NC_002947/2023339‑2023632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑