Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,316,260	Δ1 bp	6.1%	coding (64/1548 nt)	PP_2038 →	long‑chain‑fatty‑acid‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,316,260	0	G	.	6.1%	213.0 / 6.8	66	coding (64/1548 nt)	PP_2038	long‑chain‑fatty‑acid‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base G (36/26);  new base . (2/2);  total (38/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.31e-01

CATACGCAACTGGTATTACTTGCCCCGGCTTCACGCTGTTTAGCATCGATCGGGTCTTAACCGTCGACGAGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACCGCGCCGAACTGGTGGAAGCAGAGGTGGCTT  >  NC_002947/2316117‑2316407                                                                                                                                                 |                                                                                                                                                    cATACGCAACTGGTATTACTTGCCCCGGCTTCACGCTGTTTAGCATCGATCGGGTCTTAACCGTCGACGAGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGc                                                                                                                                                <  2:115379/149‑1 (MQ=255)     cGCAACTGGTATTACTTGCCCCGGCTTCACGCTGTTTAGCATCGATCGGGTCTTAACCGTCGACGAGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGTCGATC‑ACCGCcaga                                                                                                                                            <  4:214646/149‑3 (MQ=255)      gCAACTGGTATTACTTGCCCCGGCTTCACGCTGTTTAGCATCGATCGGGTCTTAACCGTCGACGAGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCacac                                                                                                                                           >  7:218784/1‑149 (MQ=255)          cTGGTATTACTTGCCCCGGCTTCACGCTGTTTAGCATCGATCGGGTCTTAACCGTCGACGAGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCgg                                                                                                                                       <  6:273843/149‑1 (MQ=255)          cTGGTATTACTTGCCCCGGCTTCACGCTGTTTAGCATCGATCGGGTCTTAACCGTCGACGAGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCgg                                                                                                                                       <  6:70462/149‑1 (MQ=255)             gTATTACTTGCCCCGGCTTCACGCTGTTTAGCATCGATCGGGTCTTAACCGTCGACGAGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCt                                                                                                                                     <  4:152198/148‑1 (MQ=255)                         ccGGCTTCACGCTGTTTAGCATCGATCGGGTCTTAACCGTCGACGAGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAg                                                                                                                        >  4:121892/1‑149 (MQ=255)                          cGGCTTCACGCTGTTTAGCATCGATCGGGTCTTAACCGTCGACGAGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCt                                                                                                                           <  6:277150/145‑1 (MQ=255)                          cGGCTTCACGCTGTTTAGCATCGATCGGGTCTTAACCGTCGACGAGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCt                                                                                                                           >  5:277150/1‑145 (MQ=255)                            gCTTCACGCTGTTTAGCATCGATCGGGTCTTAACCGTCGACGAGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTc                                                                                                                     <  8:360803/149‑1 (MQ=255)                                 aCGCTGTTTAGCATCGATCGGGTCTTAACCGTCGACGAGAACAACAACGTGACCCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGaac                                                                                                                 >  1:373213/1‑148 (MQ=255)                                 aCGCTGTTTAGCATCGATCGGGTCTTAACCGTCGACGAGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGaac                                                                                                                 >  1:192361/1‑148 (MQ=255)                                 aCGCTGTTTAGCATCGATCGGGTCTTAACCGTCGACGAGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGaac                                                                                                                 >  6:315845/1‑148 (MQ=255)                                   gCTGTTTAGCATCGATCGGGTCTTAACCGTCGACGAGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGaacaac                                                                                                              <  4:355414/149‑1 (MQ=255)                                           gCATCGATCGGGTCTTAACCGTCGACGAGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCaa                                                                                                       <  2:349315/148‑1 (MQ=255)                                                 atcgGGTCTTAACCGTCGACGAGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTg                                                                                                <  7:279683/149‑1 (MQ=255)                                                             ccGTCGACGAGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGGACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTg                                                                                    >  1:316577/1‑149 (MQ=255)                                                              cGTCGACGAGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGc                                                                                   >  4:391776/1‑149 (MQ=255)                                                              cGTCGACGAGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGc                                                                                   <  5:281682/149‑1 (MQ=255)                                                               gTCGACGAGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCt                                                                                  >  4:336919/1‑149 (MQ=255)                                                                  gacgaGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGc                                                                                                                                      >  2:137524/1‑94 (MQ=255)                                                                  gacgaGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGc                                                                                                                                      <  1:137524/94‑1 (MQ=255)                                                                       gaACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATATTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAgg                                                                          <  2:29854/149‑1 (MQ=255)                                                                       gaACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAgg                                                                          <  3:336919/149‑1 (MQ=255)                                                                       gaACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGAGGT‑GATCGAACGCCACACCCGGCTGACATTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAgg                                                                          >  1:337487/1‑149 (MQ=255)                                                                          caacaacGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAGCAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAgg                                                                          <  6:184978/146‑1 (MQ=255)                                                                          caacaacGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAGCAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAgg                                                                          >  5:184978/1‑146 (MQ=255)                                                                           aacaacGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCAt                                                                      >  6:335826/1‑149 (MQ=255)                                                                             caacGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCg                                                                    <  3:391776/149‑1 (MQ=255)                                                                                cGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTg                                                                                                >  1:256434/1‑118 (MQ=255)                                                                                cGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTg                                                                                                <  2:256434/118‑1 (MQ=255)                                                                                     aCCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGc                                                            <  5:335826/149‑1 (MQ=255)                                                                                       cTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAAACAGCTGTCCTCGGCCTTGCTGGCACAGGGCATCGCCACGTGc                                                            >  1:233805/1‑147 (MQ=255)                                                                                        ttGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAg                                                         >  8:67116/1‑149 (MQ=255)                                                                                        ttGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAg                                                         >  6:352067/1‑149 (MQ=255)                                                                                             aCCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGt                                                    >  6:350087/1‑149 (MQ=255)                                                                                                 tATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGc                                                 >  4:362047/1‑148 (MQ=255)                                                                                                   tCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCAt                                              >  5:131416/1‑149 (MQ=255)                                                                                                        tCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGc                                                        >  5:327720/1‑134 (MQ=255)                                                                                                        tCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGc                                                        <  6:327720/134‑1 (MQ=255)                                                                                                          gAAGTACCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGgcg                                       >  4:9433/1‑149 (MQ=255)                                                                                                                ccAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAAc                                 >  2:230000/1‑149 (MQ=255)                                                                                                                ccAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGCGCGAGCATGTCGCCATCCTGGCGCCCAAc                                 >  2:236219/1‑149 (MQ=255)                                                                                                                  aGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACcg                               >  6:293656/1‑149 (MQ=255)                                                                                                                  aGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACcg                               >  1:247647/1‑149 (MQ=255)                                                                                                                     tACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACCgcgc                            <  8:218784/149‑1 (MQ=255)                                                                                                                          gCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACCGCGCCGAAc                       >  7:259928/1‑149 (MQ=255)                                                                                                                          gCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACCGCGCCGAAc                       >  4:349980/1‑149 (MQ=255)                                                                                                                           ccTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACCGCGCCGAACt                      >  4:134906/1‑149 (MQ=255)                                                                                                                               taCGTCTGGCGGT‑GATCGACCGCCACACCCGTCTGACCTTCGCTCTGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACCGCGCCTAACtggt                   <  2:373213/147‑1 (MQ=255)                                                                                                                                  gtgtGGCGGTCGATC‑ACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTgg                                                                                >  2:267600/4‑84 (MQ=38)                                                                                                                                  gtgtGGCGGTCGATC‑ACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTgg                                                                                <  1:267600/81‑1 (MQ=38)                                                                                                                                  gtgtGGCGGTCGATC‑ACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACCGCGCCGAACtggtgg                 >  1:332035/4‑147 (MQ=255)                                                                                                                                   tCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACCGCGCCGAACTGGTGGAAg              >  5:83868/1‑149 (MQ=255)                                                                                                                                   tCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACCGCGCCGAACTGGTGGAAg              <  3:362047/149‑1 (MQ=255)                                                                                                                                     tGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACCGCGCCGAACTGGTGGAAg              >  1:391539/1‑147 (MQ=255)                                                                                                                                       gCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACCGCGCCGAACTGGTGGAAGCa            <  2:42919/147‑1 (MQ=255)                                                                                                                                       gCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACCGCGCCGAACTGGTGGAAGCa            >  2:4959/1‑147 (MQ=255)                                                                                                                                       gCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACCGCGCCGAACTGGTGGAAGCa            >  3:104431/1‑147 (MQ=255)                                                                                                                                       gCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCCCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACCGCGCCGAACTGGTGGAAGCag           <  5:350087/148‑1 (MQ=255)                                                                                                                                        cGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACCGCGCCGAACTGGTGGAAGCagag         <  3:9433/149‑1 (MQ=255)                                                                                                                                             gATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACCGCGCCGAACTGGTGGAAGCagag         <  1:358128/145‑1 (MQ=255)                                                                                                                                             gATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACCGCGCCGAACTGGTGGAAGCagag         >  2:358128/1‑145 (MQ=255)                                                                                                                                              aTCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACCGCGCCGAACTGGTGGAAGCAGAGGTGGc    >  2:191491/1‑149 (MQ=255)                                                                                                                                               tCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACCGCGCCGAACTGGTGGAAGCAGAGGTGGCt   <  2:391539/149‑1 (MQ=255)                                                                                                                                                cGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACCGCGCCGAACTGGTGGAAGCAGAGGTGGCtt  >  5:34950/1‑149 (MQ=255)                                                                                                                                                 gACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACCGCGCCGAACTGGTGGAAGCAGAGGTGGCtt  <  1:4959/148‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                    CATACGCAACTGGTATTACTTGCCCCGGCTTCACGCTGTTTAGCATCGATCGGGTCTTAACCGTCGACGAGAACAACAACGTGAACCTTGGAACCCTATCCAGTCGAAGTGCCAGATACTGGCCTGAACGTCTGGCGGT‑GATCGACCGCCACACCCGGCTGACCTTCGCTCAGCTCGAACAACGGGCCAACCAGCTGGCCTCGGCCTTGCTGGCACAGGGCATCGCCACGGGCGAGCATGTCGCCATCCTGGCGCCCAACCGCGCCGAACTGGTGGAAGCAGAGGTGGCTT  >  NC_002947/2316117‑2316407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑