Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 421,167:1 +A 5.7% coding (2781/2787 nt) PP_0348 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947421,1671.A 5.7% 362.7 / 14.1 88coding (2781/2787 nt)PP_0348hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (40/43);  new base A (3/2);  total (43/45)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

ACGCCACCCAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAATCTCCAAGTGGTAA  >  NC_002947/421019‑421314
                                                                                                                                                     |                                                                                                                                                   
aCGCCACCCAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTgg                                                                                                                                                      >  1:382836/1‑149 (MQ=255)
     aCCCAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCcgg                                                                                                                                                <  4:323608/149‑1 (MQ=255)
     aCCCAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCcgg                                                                                                                                                <  4:287004/149‑1 (MQ=255)
     aCCCAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCcgg                                                                                                                                                >  4:195993/1‑149 (MQ=255)
       ccAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCcggcg                                                                                                                                              <  5:386832/149‑1 (MQ=255)
       ccAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCcggcg                                                                                                                                              >  3:9183/1‑149 (MQ=255)
        cagctcagctTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCcggcgg                                                                                                                                             >  6:303554/1‑149 (MQ=255)
         agctcagctTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCggt                                                                                                                                            >  6:236610/1‑149 (MQ=255)
                  tCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCt                                                                                                                                   >  1:77074/1‑149 (MQ=255)
                   cGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCt                                                                                                                                   <  1:78767/148‑1 (MQ=255)
                      cccGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTgg                                                                                                                                 >  3:254762/1‑147 (MQ=255)
                      cccGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCa                                                                                                                               <  1:136468/149‑1 (MQ=255)
                        cGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAgt                                                                                                                             <  7:327416/149‑1 (MQ=255)
                        cGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAgt                                                                                                                             <  4:308280/149‑1 (MQ=255)
                           ccTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTc                                                                                                                          >  1:153577/1‑149 (MQ=255)
                           ccTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTc                                                                                                                          <  4:156667/149‑1 (MQ=255)
                               tGCCCGTGACCGGGCACGGCTGCTGCGCATCTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGc                                                                                                                       <  1:391529/148‑1 (MQ=255)
                                 cccGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAg                                                                                                                    >  8:315922/1‑149 (MQ=255)
                                 cccGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGC‑CCACTGACAGCGGAGCcggcg                                                                                                                                              <  5:205702/123‑1 (MQ=255)
                                 cccGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGC‑CCACTGACAGCGGAGCcggcg                                                                                                                                              >  6:205702/1‑123 (MQ=255)
                                     tGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCAc                                                                                                                <  7:83425/149‑1 (MQ=255)
                                       aCCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCAct                                                                                                               <  7:350464/148‑1 (MQ=255)
                                        ccGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCActgc                                                                                                             >  5:253826/1‑149 (MQ=255)
                                         cGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCActgct                                                                                                            <  8:157746/149‑1 (MQ=255)
                                             cACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTgcgc                                                                                                        <  2:153577/149‑1 (MQ=255)
                                              aCGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGAGCTGCCAGGGACAGCCAGGCACTGATGcccc                                                                                                       >  6:84785/1‑149 (MQ=255)
                                              aCGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGACCGCCGGC‑CCACTGACAGCGGAGCcggcggc                                                                                                                                            <  3:138122/112‑2 (MQ=37)
                                              aCGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGACCGCCGGC‑CCACTGACAGCGGAGCcggcggc                                                                                                                                            >  4:138122/1‑111 (MQ=37)
                                                     ctgcGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCGGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGGCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCcctgcct                                                                                                 <  2:339426/148‑1 (MQ=255)
                                                      tgcGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTgg                                                                                               >  1:246130/1‑149 (MQ=255)
                                                       gcgcAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGc                                                                                              <  7:315922/149‑1 (MQ=255)
                                                           aaCTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGccagt                                                                                          <  2:146830/149‑5 (MQ=255)
                                                             cTGGCCCACTGGCAGTGCCTTGCAGCAGCGGCTGGACCGGCTGTTGCCAAAACTGGGCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAAcgc                                                                                        <  4:254762/149‑1 (MQ=255)
                                                              tGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAAcgcg                                                                                       >  4:15158/1‑149 (MQ=255)
                                                                  ccACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTAt                                                                                    >  4:124728/1‑148 (MQ=255)
                                                                   cACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCa                                                                                  <  4:9183/149‑1 (MQ=255)
                                                                     cTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGc                                                                                >  2:183738/1‑149 (MQ=255)
                                                                         cAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACt                                                                            >  1:258337/1‑149 (MQ=255)
                                                                          aGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGACTGGCTGACActgcca                                                                                                           <  5:378783/117‑3 (MQ=255)
                                                                          aGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGACTGGCTGACActgcca                                                                                                           >  6:378783/1‑115 (MQ=255)
                                                                              ccTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCg                                                                       >  1:361754/1‑149 (MQ=255)
                                                                                  gCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCGGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGttt                                                                    <  7:136073/148‑1 (MQ=255)
                                                                                  gCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGtttt                                                                   >  1:79882/1‑149 (MQ=255)
                                                                                    aTCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCg                                                                 >  1:370411/1‑149 (MQ=255)
                                                                                     tCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGt                                                                >  3:136506/1‑149 (MQ=255)
                                                                                     tCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGt                                                                >  2:151802/1‑149 (MQ=255)
                                                                                       aGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTTGCGAACGCGTATCAGCCACTGGCCGTTTTCGTgg                                                              <  2:387909/149‑1 (MQ=255)
                                                                                        gCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGgc                                                             >  8:141195/1‑149 (MQ=255)
                                                                                        gCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGgc                                                             <  1:183738/149‑1 (MQ=255)
                                                                                             tGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCgcagc                                                        >  2:387058/1‑149 (MQ=255)
                                                                                               gACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCgcagca                                                       <  1:20803/148‑1 (MQ=255)
                                                                                                 ccGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGcc                                                                        >  3:84711/1‑129 (MQ=255)
                                                                                                 ccGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGcc                                                                        <  4:84711/129‑1 (MQ=255)
                                                                                                 ccGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCAcca                                                    <  3:360487/149‑2 (MQ=255)
                                                                                                   ggCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGcc                                                  <  3:124728/149‑1 (MQ=255)
                                                                                                          gCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGca                                           >  4:362826/1‑149 (MQ=255)
                                                                                                          gCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGca                                           >  3:194557/1‑149 (MQ=255)
                                                                                                           ccAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGcac                                          <  2:382836/149‑1 (MQ=255)
                                                                                                           ccAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGcac                                          >  8:111531/1‑149 (MQ=255)
                                                                                                            cAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGcacc                                         >  8:53296/1‑149 (MQ=255)
                                                                                                            cAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGcacc                                         >  2:41561/1‑149 (MQ=255)
                                                                                                            cAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGcac                                          >  4:147304/1‑148 (MQ=255)
                                                                                                             aaaaCTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGcacca                                        >  8:128097/1‑149 (MQ=255)
                                                                                                             aaaaCTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGACGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGcacca                                        >  6:415376/1‑149 (MQ=255)
                                                                                                                    gTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACcgcg                                   <  6:287811/147‑1 (MQ=255)
                                                                                                                    gTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGc                                 <  3:362826/149‑1 (MQ=255)
                                                                                                                     tCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCg                                >  5:360153/1‑149 (MQ=255)
                                                                                                                       gACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGt                               <  2:258337/148‑1 (MQ=255)
                                                                                                                       gACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCACTGTCAGCCAGTCACTGCTGCGCCCTGCCTGACGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGt                               <  5:415376/148‑1 (MQ=255)
                                                                                                                        aCTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTg                             <  5:303554/149‑1 (MQ=255)
                                                                                                                         cTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGc                            <  3:269327/149‑1 (MQ=255)
                                                                                                                          tGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCt                           >  8:41074/1‑149 (MQ=255)
                                                                                                                            aGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCt                           <  2:77074/147‑1 (MQ=255)
                                                                                                                            aGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTg                          <  5:236610/148‑1 (MQ=255)
                                                                                                                            aGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTg                          <  2:246130/148‑1 (MQ=255)
                                                                                                                             ggCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGc                        <  7:128097/149‑1 (MQ=255)
                                                                                                                             caccgccgGC‑CCCCTGACAGCGGAGCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTgg                         >  1:257650/3‑148 (MQ=255)
                                                                                                                                cgccgGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAAc                     <  6:253826/149‑1 (MQ=255)
                                                                                                                                cgccgGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAAc                     >  5:335999/1‑149 (MQ=255)
                                                                                                                                       tCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACgg                   <  7:182834/144‑1 (MQ=255)
                                                                                                                                       tCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACgg                   >  8:182834/1‑144 (MQ=255)
                                                                                                                                       tCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAAtc              >  2:315229/1‑149 (MQ=255)
                                                                                                                                         cGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAAtctc            >  6:15682/1‑149 (MQ=255)
                                                                                                                                         cGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAAtctc            >  7:152724/1‑149 (MQ=255)
                                                                                                                                            tGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAATCTCCaa         <  2:370411/149‑1 (MQ=255)
                                                                                                                                              tCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCTACGGCACCACCCCGGCGTTGCTGGCAACGGCAATCTCCAAg        <  2:79882/148‑1 (MQ=255)
                                                                                                                                              tCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAATCTCCaa         <  5:241028/147‑1 (MQ=255)
                                                                                                                                               cAGGGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAATCTCCAAGTg      <  5:84785/149‑1 (MQ=255)
                                                                                                                                                aGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAATCTCCAAGTgg     >  2:190128/1‑149 (MQ=255)
                                                                                                                                                    g‑gCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAATCTCCAAGTGGTaa  <  7:111531/148‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ACGCCACCCAGCTCAGCTTCGCCCCGGCCTATGCCCGTGACCGGGCACGGCTGCTGCGCAACTGGCCCACTGGCAGTGCCTTGCATCAGCGGCTGGACCGGCTGTTGCCAAAACTGGTCGACTGAGGCCGCCGGCTCCGCTGTCAGTGG‑GCCGGCGGTGGTTGATCTGGCAGTGTCAGCCAGTCACTGCTGCGCCCTGCCTGGCGAACGCGTATCAGCCACTGGCCGTTTTCGTGGCGCAGCACCGCCCACGGCACCACCCCGGCGTTGCTGGCAACGGCAATCTCCAAGTGGTAA  >  NC_002947/421019‑421314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑