Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,925,579:1	+C	9.6%	coding (566/1083 nt)	gcvT‑II ←	aminomethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,925,579	1	.	C	9.6%	304.1 / 26.8	83	coding (566/1083 nt)	gcvT‑II	aminomethyltransferase
Reads supporting (aligned to +/- strand):  ref base . (36/39);  new base C (3/5);  total (39/44)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAGAT  >  NC_002947/5925432‑5925726                                                                                                                                                      |                                                                                                                                                    tCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCgg                                                                                                                                                      >  3:121507/1‑148 (MQ=255)   cTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑ta                                                                                                                                                   >  2:239114/1‑148 (MQ=255)   cTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCAGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑ta                                                                                                                                                   >  3:160827/1‑148 (MQ=255)    tGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑ta                                                                                                                                                   >  7:363292/1‑147 (MQ=255)      gCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGc                                                                                                                                               <  4:395872/149‑1 (MQ=255)       ccGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGcc                                                                                                                                              <  8:40439/149‑1 (MQ=255)             aGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGGCTATA‑CCGGTgag                                                                                                                                        <  5:137984/149‑3 (MQ=255)             aGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCg                                                                                                                                        <  4:125846/149‑1 (MQ=255)              ggTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCgg                                                                                                                                       <  2:270230/149‑1 (MQ=255)               gTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCggg                                                                                                                                      >  5:298909/1‑149 (MQ=255)               gTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCggg                                                                                                                                      <  5:319323/149‑1 (MQ=255)               gTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCggg                                                                                                                                      <  1:25578/149‑1 (MQ=255)               gTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACAAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCggg                                                                                                                                      >  6:3216/1‑149 (MQ=255)                 tCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGGCTATA‑CCGGTGAggacg                                                                                                                                    >  8:20449/1‑146 (MQ=255)                 tCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGGCTATA‑CCGGTGAggacg                                                                                                                                    >  8:14562/1‑146 (MQ=255)                  cATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGGCTATA‑CCGGTGAggacg                                                                                                                                    <  7:14562/148‑4 (MQ=255)                  cATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGGCTATA‑CCGGTGAggacg                                                                                                                                    <  7:20449/148‑4 (MQ=255)                    tGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑t                                                                                                                                                    >  5:171178/1‑130 (MQ=255)                    tGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑t                                                                                                                                                    <  6:171178/130‑1 (MQ=255)                    tGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAAt                                                                                                                                  >  7:92677/1‑148 (MQ=255)                      ccGGCTTCCAGGCGCAGGGTATCGCTGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGGCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGa                                                                                                                                <  3:326084/148‑1 (MQ=255)                      ccGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGaa                                                                                                                               >  6:265008/1‑149 (MQ=255)                      ccGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGaa                                                                                                                               <  6:271400/149‑1 (MQ=255)                      ccGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCGCACCGGCTATA‑CCGGTGAggacggcct                                                                                                                                <  6:303472/148‑8 (MQ=255)                      ccGGCTTCCAGGCACAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGaa                                                                                                                               <  7:64748/149‑1 (MQ=255)                         gCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAAc                                                                                                                              >  1:402054/1‑147 (MQ=255)                           ttCCAGGCGCAGGGAATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATATC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCACTGAACAAGt                                                                                                                          >  7:346743/1‑149 (MQ=255)                                 gcgcAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGGCTATA‑CCGGTga                                                                                                                                         >  2:387287/1‑127 (MQ=255)                                 gcgcAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGGCTATA‑CCGGTga                                                                                                                                         <  1:387287/128‑2 (MQ=255)                                 gcgcAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGt                                                                                                                    <  6:35780/149‑1 (MQ=255)                                   gcAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCTCCGACCAGGTCGTTGAAGAAGGCCACCTCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGTCAATGAACCAGTCGCCGTCg                                                                                                                  <  4:132315/149‑1 (MQ=255)                                     aGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGc                                                                                                                <  5:183449/149‑1 (MQ=255)                                             gcgGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCTGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGccc                                                                                                         <  4:160827/148‑1 (MQ=255)                                             gcgGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCt                                                                                                        >  6:188507/1‑149 (MQ=255)                                                  cgcCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAAc                                                                                                   <  5:265008/149‑1 (MQ=255)                                                   gcCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCATCCTCACCGG‑TATAGCCGGTGCGGGCAATGaa                                                                                                                               <  3:300052/120‑1 (MQ=255)                                                   gcCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCATCCTCACCGG‑TATAGCCGGTGCGGGCAATGaa                                                                                                                               >  4:300052/1‑120 (MQ=255)                                                    cccAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACgg                                                                                                 >  3:116625/1‑149 (MQ=255)                                                         gCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCa                                                                                            <  6:24260/149‑1 (MQ=255)                                                         gCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCa                                                                                            <  5:188507/149‑1 (MQ=255)                                                         gCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCAGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGgcg                                                                                              >  2:312432/1‑147 (MQ=255)                                                                 gggCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTcgcg                                                                                     <  4:185441/148‑1 (MQ=255)                                                                  ggCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCg                                                                                                                        >  8:167731/1‑112 (MQ=255)                                                                  ggCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCg                                                                                                                        <  7:167731/112‑1 (MQ=255)                                                                  ggCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCggc                                                                                   <  8:363292/149‑2 (MQ=255)                                                                  ggCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGa                                                                                   <  6:298909/149‑1 (MQ=255)                                                                   gCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTCGGAGGCCGTCATAACCGG‑TTAACCGGGTGCGGGCAATGAACAAGTCGCCGACGGAAACCCCCACCAACGGGCGCCGTTCCCGGa                                                                                   >  1:325526/1‑149 (MQ=255)                                                                   gCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCACCGCCCTCGAACGGGCGCAGTTCGCGGAt                                                                                  >  8:230392/1‑149 (MQ=255)                                                                   gCGATACCGGCGCCGACAAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGAt                                                                                  <  5:3216/149‑1 (MQ=255)                                                                    cGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGGCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGAt                                                                                  >  1:25979/1‑148 (MQ=255)                                                                     gATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGa                                                                                <  2:283029/149‑1 (MQ=255)                                                                          cGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAg                                                                                                                           >  7:41882/1‑101 (MQ=255)                                                                          cGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAg                                                                                                                           <  8:41882/101‑1 (MQ=255)                                                                                 gACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAgcgcgcg                                                                    >  3:333769/1‑149 (MQ=255)                                                                                           ttGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAg                                                          >  5:276877/1‑149 (MQ=255)                                                                                             gaagaaGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAg                                                          >  3:202206/1‑147 (MQ=255)                                                                                             gaagaaGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAg                                                          >  3:153671/1‑147 (MQ=255)                                                                                               agaagGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAgg                                                      <  2:25979/149‑1 (MQ=255)                                                                                                 aagGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGGCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGgc                                                    <  7:230392/149‑1 (MQ=255)                                                                                                     ccACCGCCTGATCGCCCTGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGgccgcc                                                <  1:288979/149‑1 (MQ=255)                                                                                                      cACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCa                                               >  2:312430/1‑149 (MQ=255)                                                                                                       aCCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCAc                                              >  3:200609/1‑149 (MQ=255)                                                                                                       aCCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCAAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCAc                                              >  6:117817/1‑149 (MQ=255)                                                                                                              gATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTcg                                       >  7:300192/1‑149 (MQ=255)                                                                                                               aTCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTcgc                                      <  4:153671/149‑1 (MQ=255)                                                                                                               aTCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTcgc                                      <  2:14150/149‑1 (MQ=255)                                                                                                                tCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTcgcg                                     <  1:239114/149‑1 (MQ=255)                                                                                                                  gCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCgg                                    <  1:312432/148‑1 (MQ=255)                                                                                                                   cccGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCggg                                   >  1:114836/1‑148 (MQ=255)                                                                                                                   cccGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCGGAGCAGGGCCGCCACCTTTTCGCGGGc                                  >  7:294284/1‑149 (MQ=255)                                                                                                                       ggAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGACCCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCAcgc                              <  7:138065/149‑1 (MQ=255)                                                                                                                          aGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGgccgcc                                                <  7:156423/128‑1 (MQ=255)                                                                                                                          aGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGgccgcc                                                >  8:156423/1‑128 (MQ=255)                                                                                                                           gatgatCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCCCGCGGcc                          >  8:330884/1‑149 (MQ=255)                                                                                                                              gatCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCt                       <  8:294284/149‑1 (MQ=255)                                                                                                                                  tc‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCttt                                          >  7:21133/1‑126 (MQ=255)                                                                                                                                  tc‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCttt                                          <  8:21133/126‑1 (MQ=255)                                                                                                                                   c‑cAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATg                  >  1:363210/1‑149 (MQ=255)                                                                                                                                   c‑cAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATg                  >  4:54910/1‑149 (MQ=255)                                                                                                                                     cAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGAt                   <  4:202206/147‑1 (MQ=255)                                                                                                                                     cAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATg                  <  2:402054/148‑1 (MQ=255)                                                                                                                                     cAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATg                  <  4:333769/148‑1 (MQ=255)                                                                                                                                       ggCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGc                >  2:77776/1‑148 (MQ=255)                                                                                                                                          cGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAg             >  1:313552/1‑148 (MQ=255)                                                                                                                                               tCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACACCATCGAAAGGACGAAGTTCGGGGATGAGTGCAGCGCGCGCGGGGATGAGCAGGGCCGCAACCTTTTCGCGGGCACGCGGCCCCAGGATCGCAAGGATGGc       >  8:255966/1‑149 (MQ=255)                                                                                                                                                cACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGc       >  5:59465/1‑148 (MQ=255)                                                                                                                                                cACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGc       >  3:340438/1‑148 (MQ=255)                                                                                                                                                 aCCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGc       >  8:305138/1‑147 (MQ=255)                                                                                                                                                  ccGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAg    <  7:305138/149‑1 (MQ=255)                                                                                                                                                     g‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAGAt  >  3:251042/1‑148 (MQ=255)                                                                                                                                                      |                                                                                                                                                    TCCTGGCCGTACAGGTTCATGCCGGCTTCCAGGCGCAGGGTATCGCGGGCGCCCAGGCCGCTTGGGGCGATACCGGCGCCGACCAGGTCGTTGAAGAAGGCCACCGCCTGATCGCCCGGGAAGATGATCTC‑CAGGCCGTCCTCACCGG‑TATAGCCGGTGCGGGCAATGAACCAGTCGCCGTCGGCAACGCCCTCGAACGGGCGCAGTTCGCGGATGAGTGCAGCGCGCGCGGGGCTGAGCAGGGCCGCCACCTTTTCGCGGGCACGCGGCCCCTGGATGGCAAGGATGGCCAGAT  >  NC_002947/5925432‑5925726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑