| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,070,246:1 | +GA | 100% | intergenic (+46/+96) | aroP‑I → / ← PP_0928 | aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,070,246 | 1 | . | G | 94.7% | 54.1 / ‑2.7 | 19 | intergenic (+46/+96) | aroP‑I/PP_0928 | aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (6/12); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | NC_002947 | 1,070,246 | 2 | . | A | 94.7% | 52.3 / ‑2.7 | 19 | intergenic (+46/+96) | aroP‑I/PP_0928 | aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (6/12); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGT‑AAGGGGTATGCCGTTAGGTTTTCGGCGCCTGT‑‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACA > NC_002947/1070099‑1070385 || cTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGT‑AAGGGGTATGCCGTTAGGTTTTCGGCGCCtgt > 5:360606/1‑148 (MQ=255) gccgATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGT‑AAGGGGTATGCCGTTAGGTTTTCATCGCCTGT‑‑Gagatcgag > 5:180035/1‑142 (MQ=255) tctcGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGACTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGT‑AAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCgcggg > 7:135866/1‑149 (MQ=255) ggTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGACTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGT‑AAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCgcggg < 8:135866/145‑1 (MQ=255) aTGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTCAAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCt < 1:115452/149‑1 (MQ=38) aTGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGT‑AAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCt < 4:372215/148‑1 (MQ=39) aTGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGT‑AAGGGGCATGCCTTTAGGCTTTCATCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCt < 2:304199/148‑1 (MQ=25) tGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGT‑AAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCg < 6:360606/149‑1 (MQ=38) cTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGT‑AAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGa < 5:287242/148‑1 (MQ=255) cTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGT‑AAGGGGTATGCCGTTAGGTTTTCATCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGAt < 6:180035/149‑1 (MQ=37) cTGATCCCGGCATGGATCGCTGTGCTGGGGGTGGCTTTCAAGCTGAAGACGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGT‑AAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGAt < 7:185734/149‑1 (MQ=38) cGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGT‑AAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAg < 8:108842/149‑1 (MQ=255) tttCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGT‑AAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGAt < 8:313891/114‑1 (MQ=37) tttCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGT‑AAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGAt > 7:313891/1‑114 (MQ=37) gCTCGCCAGGCCAAGGGCTGCTCGGGTTTGTTCGT‑AAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCt < 1:22318/94‑1 (MQ=25) gCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGT‑AAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCt > 2:22318/1‑94 (MQ=25) aTCGGGTTTGTTCGT‑AAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCgcgggcg > 7:378842/1‑69 (MQ=21) aTCGGGTTTGTTCGT‑AAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCgcgggcg < 8:378842/69‑1 (MQ=21) gTTCGT‑AAGGGGTATGCCGTTAGGTTTTCGGCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTcc > 5:210037/1‑149 (MQ=255) gcgcCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAaca > 3:58317/1‑149 (MQ=255) || CTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGT‑AAGGGGTATGCCGTTAGGTTTTCGGCGCCTGT‑‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACA > NC_002947/1070099‑1070385 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |