| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,126,645:1 | +C | 100% | intergenic (‑99/+50) | tdcG‑II ← / ← gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,126,645 | 1 | . | C | 87.0% | 62.3 / 5.1 | 23 | intergenic (‑99/+50) | tdcG‑II/gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (3/0); new base C (9/11); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGG‑TAAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCA > NC_002947/1126507‑1126784 | cgatgccgatCTTGAACAGGTCGATGACGCGCAGTGACTTGTGGACCTCCTGCCAGGTTGTGGGGTGGACATTTAGCCGTTTAGGCGC‑GGGTTTACCCGCTAATGGGGAGGTATAGCCACCATCGCATTCG‑G‑GGG‑TAAACCCGCTCCTa < 4:244344/149‑1 (MQ=255) aGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTTACCGCGCATGGGGAGGTATAGCCCCCATCGCATTAG‑C‑GGGTTAAA‑CCGCTCCGACACACAGCCCGcccccc > 2:276351/1‑149 (MQ=255) aCATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTAACCCCGCATGCGGAGGTTTATCCCCCAACGCATTAG‑CGGGG‑TAAC‑CCGCTCCTACACACAGCCCGCCCCCCCACCCCGGTGGCGgggggg > 5:180832/1‑149 (MQ=255) ctcctGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGGTTTAACCCCAAATGCGAAGG‑ATATCCCCCAAACCAATAGCC‑GGG‑TAAA‑CCCCCACAAAACAAACCCCCCCCCCCAAACCAAGGGGGGGGGGGGGCGATAAGGc > 2:322886/1‑149 (MQ=255) tcctGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCaa < 7:64071/121‑1 (MQ=255) cctGCCAGGTTGTTGGGTGGACATTGCGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGt < 5:390661/149‑1 (MQ=255) ggTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATa < 1:276351/149‑1 (MQ=255) gcgc‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCa < 1:322886/149‑1 (MQ=255) gc‑gGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAgg > 1:267860/1‑149 (MQ=255) gggTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGtt > 1:275290/1‑149 (MQ=255) gTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGc > 8:4212/1‑149 (MQ=255) cccGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCg > 6:9274/1‑149 (MQ=255) ccGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc > 5:401829/1‑149 (MQ=255) cgcgAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGAt < 5:59879/106‑1 (MQ=255) cgcgAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGAt > 6:59879/1‑106 (MQ=255) gTATAGCCACCATCGCATTCG‑C‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCCCACCAGGTTGCGGTCGCCGAACACGTTGTc > 5:240563/1‑148 (MQ=255) gTATAGCCACCATCGCATTCG‑C‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTc > 2:198059/1‑148 (MQ=255) caccaTCGCATTCG‑C‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGc < 2:275290/149‑1 (MQ=255) cATTCG‑C‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGcaca > 7:36881/1‑105 (MQ=255) cATTCG‑C‑GGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGcaca < 8:36881/105‑1 (MQ=255) cg‑c‑gGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCggcggc < 2:267860/148‑1 (MQ=255) g‑c‑gGG‑TAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCa < 6:401829/149‑1 (MQ=255) g‑c‑gGG‑TAAACCCGCGCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGCCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCa < 5:252258/149‑1 (MQ=255) | CGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGG‑TAAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCA > NC_002947/1126507‑1126784 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |