| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 278,742:1 | +A | 100% | intergenic (‑237/+77) | PP_0224 ← / ← sctC | DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 278,742 | 1 | . | A | 97.4% | 121.2 / ‑3.0 | 39 | intergenic (‑237/+77) | PP_0224/sctC | DszC family monooxygenase/sulfur compound ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (16/22); total (16/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑T‑AACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGG > NC_002947/278596‑278881 | tGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑T‑ag < 2:85280/149‑2 (MQ=255) gCTACAGCTGATCGCGAAAGACGTGTGCTGGTCCGGTTGTCCGGTATATATACCCTAATTATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑T‑aaac < 7:254542/147‑3 (MQ=255) cAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCt < 5:338937/148‑1 (MQ=255) gcgACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTAcacaca > 2:269580/1‑149 (MQ=255) gACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTacaca < 3:250357/145‑1 (MQ=255) gACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTacaca > 4:250357/1‑145 (MQ=255) cTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCt < 7:273753/148‑1 (MQ=255) tggtccggtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGGAA‑AACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTa < 5:282942/149‑1 (MQ=255) gtATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCgg < 6:239156/149‑1 (MQ=255) gtATATATACCCTAATGAATTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCgg < 5:25712/149‑1 (MQ=255) tatatataCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCggg < 5:19303/149‑1 (MQ=255) tataCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTa > 6:183791/1‑149 (MQ=255) taCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTAcc > 6:86262/1‑149 (MQ=255) aCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATACCTCAAGATCTGTGCACTGt < 6:82897/133‑1 (MQ=255) aCCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATACCTAAAGATCTGTGCACTGt > 5:82897/1‑133 (MQ=255) ccTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCt > 6:294092/1‑122 (MQ=255) ccTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCt < 5:294092/122‑1 (MQ=255) ccTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTa > 7:116157/1‑132 (MQ=255) ccTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTa < 8:116157/132‑1 (MQ=255) ccTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTAAACACATGCCTCAAGATATGTGCACTGTAGGAGCGGGTTTACCcgc > 3:161757/1‑149 (MQ=255) tAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTACTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCcgcg > 8:77144/1‑148 (MQ=255) ttATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTaca < 8:134764/96‑1 (MQ=255) ttATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTaca > 7:134764/1‑96 (MQ=255) ataAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCg > 3:177685/1‑148 (MQ=255) ataAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTACTACCCACATGCCTCAAGAGCAGTGCCCGGTAGGAGCGGGTTTACGCGCGAAAGGGCCgg > 8:101472/1‑149 (MQ=255) tttATGAACTAACTTTTTGGAATATTTATAGACGGGGGAGTTGTTACCGAGGCGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCCCTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGt < 5:344651/148‑1 (MQ=38) tGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCaa > 7:224564/1‑94 (MQ=255) tGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCaa < 8:224564/94‑1 (MQ=255) aactTTTTGGAATATTTATAGACGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAACATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAgg < 3:74371/149‑1 (MQ=255) ttGTAATATTTATAGAAGGGGTATTTGTTACAGAGGGGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACAAATGCCTCAAGATCTGTGCACTTTAGGAGCGGTTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCa < 4:161757/148‑1 (MQ=38) ttATAGACGGGGTATTTGTTACAGCGGTGCCGGGCTCTTCGGGGG‑TAAACCCGCTCCTACACACATGCCTAAATATCTGTCCACTTTTGGAGGGGTTTAACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAgg < 4:177685/148‑1 (MQ=255) tataGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGa < 5:183791/148‑1 (MQ=255) ttACAGAGGTGCCGGGCGCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGcc < 5:86262/149‑1 (MQ=255) agagGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGt < 2:268471/65‑1 (MQ=255) agagGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGt > 1:268471/1‑65 (MQ=255) agagGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGcc > 7:112495/1‑145 (MQ=255) agagGTGCCGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGATTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGcc < 8:112495/145‑1 (MQ=255) ccGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGctct < 2:70664/149‑1 (MQ=255) ccGGGCTCTTCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGctc < 7:77144/148‑1 (MQ=255) gCTCTTCGCGGG‑TAAACCCGCTCCTACACCCATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTcttcct < 7:101472/149‑1 (MQ=255) ttCGCGGG‑TAAACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCgg > 5:324802/1‑149 (MQ=255) | TGTCGCTACAGCTGATCGCGACAGACGTGTGCTGGTCCGGTGGTCCGGTATATATACCCTAATGATTTATAAAATTTTTATGAACTAACTTTTTGGAATATTTATAGAAGGGGGATTTGTTACAGAGGTGCCGGGCTCTTCGCGGG‑T‑AACCCGCTCCTACACACATGCCTCAAGATCTGTGCACTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGAACAGGTTTACTCGGAGGCCGCAGTCCCGAGGAACTTGCGCAAGAACTGCCGCGTACGCTCTTCCTTCGG > NC_002947/278596‑278881 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |