| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,845,703:1 | +G | 100% | intergenic (‑70/+28) | PP_3394 ← / ← PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,845,703 | 1 | . | G | 93.9% | 101.8 / 0.4 | 33 | intergenic (‑70/+28) | PP_3394/PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (17/14); total (17/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑G‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAG‑CCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGC > NC_002947/3845557‑3845842 | gctgctGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑g‑g > 8:246287/1‑149 (MQ=255)gctgctGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑g‑g < 8:165328/149‑1 (MQ=255) ctgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑g‑gg < 7:358482/149‑2 (MQ=255) tgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑g‑ggc > 7:167959/1‑147 (MQ=255) tCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATc > 2:146466/1‑149 (MQ=255) tCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAg > 2:101871/1‑149 (MQ=255) aCTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGc < 7:246287/148‑1 (MQ=255) cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGAt < 6:318927/149‑1 (MQ=255) cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGAt < 7:12062/149‑1 (MQ=255) ctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGAt > 8:367741/1‑147 (MQ=255) tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTc‑gc‑g < 8:231785/118‑1 (MQ=255) tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTc‑gc‑g > 7:231785/1‑118 (MQ=255) cATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACg > 3:120897/1‑133 (MQ=255) cATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACg < 4:120897/133‑1 (MQ=255) gCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGAt > 3:286014/1‑129 (MQ=255) gCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGAt < 4:286014/129‑1 (MQ=255) attgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCActgtgc > 1:348854/1‑144 (MQ=255) attgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACgcag > 1:74163/1‑148 (MQ=255) gttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACgcagcag > 7:40117/1‑147 (MQ=255) ttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCaa > 1:263370/1‑147 (MQ=255) ttgtCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGCTGGATCAGCCCGCGCCCTGCCAGGCCCGGGCACGGAGCAACAggtggt > 4:321311/1‑149 (MQ=255) tgtCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggtggt > 4:346329/1‑148 (MQ=255) tCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTc > 1:98550/1‑147 (MQ=255) gTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGgcgc < 7:367741/149‑1 (MQ=255) ggtctcAAGGGTGGCGCCTGACAACGAGCTGTTTGAGGGGCATAAACCAGGTGTTGCGGG‑G‑GCTCATCCAGCGGCCCCCACGATGTATAACCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgag‑cc < 8:32752/147‑1 (MQ=255) aaGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAG‑CCGACAGc < 3:346329/148‑1 (MQ=255) gtgTTGCGCAATATTAAGAGCTGTTTTACGGGCTTAAAATAGGTTTTTG‑GC‑GTGCTTATCCATCGTCAGCAAGTTTGTATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAG‑CCGACAGCga < 2:263370/146‑1 (MQ=255) gACTAAGAGCTGTATGACGGCCATAAACGACGTGTTTC‑GCGG‑GCTGATCCATGGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAG‑CCGACAGCGAGAGCGTGTGCCg < 6:294189/149‑1 (MQ=255) gACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGAAGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTAAAGCAGGCGCCGAG‑CCGACAGCGAGAGCGTGTGCCg > 5:294189/1‑149 (MQ=255) cTGGATGACGGGCAGAAAAGCCGTGTTGC‑GCGG‑GCTGATCCATCGGCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAG‑CCGACAGCGAGAGCGTGTGCCGGTCGCGCAt < 3:321311/149‑1 (MQ=255) tGGATGACGGGCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAG‑CCGACAGCGAGAGCGTGTGCCGGTCGCGCATg > 2:244665/1‑149 (MQ=255) aTGACGGGCCGAAAAGACGTGTTTC‑GCGG‑GCTTATCCATCGTCAGCCACGATGGAGCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAG‑CCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAg < 8:116731/149‑1 (MQ=255) gggCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAG‑CCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGAc < 1:244665/149‑1 (MQ=255) gggCAGAAAAGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAG‑CCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGAc > 6:411893/1‑149 (MQ=255) aaGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAG‑CCGACAGCGAGAGCGTGTGCCGGTcgc < 5:191498/128‑1 (MQ=255) aaGACGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAG‑CCGACAGCGAGAGCGTGTGCCGGTcgc > 6:191498/1‑128 (MQ=255) aCGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTc < 1:88590/145‑1 (MQ=255) aCGTGTTTC‑GCGG‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAG‑CCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTc > 2:88590/1‑144 (MQ=255) gtgtTTC‑GCGG‑GCTGATCCATCGTCAGCAACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAG‑CCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAgc < 1:101871/149‑1 (MQ=255) | GCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTC‑GC‑G‑GCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAG‑CCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGC > NC_002947/3845557‑3845842 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |