Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,042,321 C→A 7.4% L137I (CTC→ATC) ‡ PP_3563 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,042,3210CA 7.4% 286.0 / 8.7 89L137I (CTC→ATC) ‡PP_3563hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (39/43);  new base A (4/3);  total (43/46)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

AGCCTGTCGGGGCCACGCGACGCACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGCACAGTGCTGGCCGGG  >  NC_002947/4042177‑4042464
                                                                                                                                                 |                                                                                                                                               
aGCCTGTCGGGGCCACGCGACGCACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCa                                                                                                                                              <  2:12143/148‑1 (MQ=255)
  ccTGTCGGGGCCACGCGACGCACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCg                                                                                                                                           >  2:329459/1‑149 (MQ=255)
   cTGTCGGGGCCACGCGACGCACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCgg                                                                                                                                          >  7:279030/1‑149 (MQ=255)
      tCGGGGCCACGCGACGGACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGTTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGgcc                                                                                                                                        <  8:262542/148‑1 (MQ=255)
      tCGGGGCCACGCGACGCACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGgccg                                                                                                                                       >  2:58324/1‑149 (MQ=255)
      tCGGGGCCACGCGACGCACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGgc                                                                                                                                         <  6:31888/147‑1 (MQ=255)
       cGGGGCCACGCGACGCACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGgccgc                                                                                                                                      >  2:274026/1‑149 (MQ=255)
        ggggCCACGCGACGCACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGAGCAGCGgccgt                                                                                                                                      <  5:401648/148‑2 (MQ=255)
            ccACGCGACGCACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTggg                                                                                                                                 <  1:29089/149‑1 (MQ=255)
            ccACGCGACGCACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTggg                                                                                                                                 <  1:317236/149‑1 (MQ=255)
                 cgACGCACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGTATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACt                                                                                                                            <  1:78064/149‑1 (MQ=255)
                     gcacgcGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCa                                                                                                                        <  7:331838/149‑1 (MQ=255)
                        cgcgCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAgg                                                                                                                      >  3:137595/1‑148 (MQ=255)
                        cgcgCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAgg                                                                                                                      <  4:137595/148‑1 (MQ=255)
                         gcgcCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGcc                                                                                                                    >  1:288639/1‑149 (MQ=255)
                           gccgccATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGa                                                                                                                  <  2:288639/149‑1 (MQ=255)
                            ccgccATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAg                                                                                                                 <  7:290646/149‑1 (MQ=255)
                            ccgccATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAg                                                                                                                 >  1:178214/1‑149 (MQ=255)
                               ccATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCGGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGa                                                                                                              >  7:106341/1‑149 (MQ=255)
                                 atgcatgcCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACa                                                                                                            <  8:378559/149‑1 (MQ=255)
                                   gcatgcCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAg                                                                                                           >  7:24004/1‑148 (MQ=255)
                                     atgcCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGGGGCCGCCTGGGCGAGTGGCAGGCCGAGGGACAGAAg                                                                                                        <  5:91867/149‑1 (MQ=255)
                                     atgcCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAg                                                                                                        <  2:178214/149‑1 (MQ=255)
                                         cATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGtgc                                                                                                    >  3:53196/1‑149 (MQ=255)
                                          aTCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGtgct                                                                                                   >  6:56141/1‑149 (MQ=255)
                                           tCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGtgctg                                                                                                  >  1:16118/1‑149 (MQ=255)
                                             gAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCt                                                                                                                                    <  3:198746/113‑1 (MQ=255)
                                             gAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCt                                                                                                                                    >  4:198746/1‑113 (MQ=255)
                                              aGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAgg                                                                                                                <  5:136224/132‑1 (MQ=255)
                                              aGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAgg                                                                                                                >  6:136224/1‑132 (MQ=255)
                                                 aaaGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCaggag                                                                                            >  5:54931/1‑149 (MQ=255)
                                                  aaGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCaggagg                                                                                           <  2:346258/149‑1 (MQ=255)
                                                   aGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGt                                                                                          >  3:26582/1‑149 (MQ=255)
                                                    gCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGt                                                                                          >  4:304640/1‑148 (MQ=255)
                                                    gCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTc                                                                                         <  1:58324/149‑1 (MQ=255)
                                                         tGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAAcgc                                                                                    >  4:61513/1‑149 (MQ=255)
                                                         tGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAAcgc                                                                                    >  4:303234/1‑149 (MQ=255)
                                                                  gtgCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAg                                                                                                                                             <  6:259679/83‑1 (MQ=255)
                                                                  gtgCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAg                                                                                                                                             >  5:259679/1‑83 (MQ=255)
                                                                    gCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGGCCCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAg                                                                          <  1:329459/149‑1 (MQ=255)
                                                                    gCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAgg                                                                         >  5:72078/1‑149 (MQ=255)
                                                                     cTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGt                                                                        <  5:56141/149‑1 (MQ=255)
                                                                     cTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGATCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGt                                                                        >  2:38414/1‑149 (MQ=255)
                                                                      tCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTg                                                                       >  3:259724/1‑149 (MQ=255)
                                                                        gACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGc                                                                      >  1:155134/1‑148 (MQ=255)
                                                                          cccGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCAc                                                                   <  8:24004/149‑1 (MQ=255)
                                                                           cggCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACg                                                                  <  8:106341/147‑1 (MQ=255)
                                                                            cGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTg                                                                                                                           >  1:397482/1‑91 (MQ=255)
                                                                            cGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTg                                                                                                                           <  2:397482/91‑1 (MQ=255)
                                                                            cGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACgg                                                                 >  1:188539/1‑149 (MQ=255)
                                                                            cGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACgg                                                                 <  8:279030/149‑1 (MQ=255)
                                                                            cGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACgg                                                                 <  1:353828/149‑1 (MQ=255)
                                                                                aTTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAAt                                                             >  5:355813/1‑149 (MQ=255)
                                                                                  tcgtcgATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATgc                                                           >  3:8693/1‑149 (MQ=255)
                                                                                    gtcgATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATgcg                                                          <  4:53196/148‑1 (MQ=255)
                                                                                    gtcgATATCGGCGGCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATgcgg                                                         <  7:381807/149‑1 (MQ=255)
                                                                                        atatCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCggcggc                                                     <  1:208614/149‑1 (MQ=255)
                                                                                           tCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGTCAATGCGGCGGCCAg                                                  >  8:67204/1‑149 (MQ=255)
                                                                                           tCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAg                                                  <  3:61513/149‑1 (MQ=255)
                                                                                           tCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAg                                                  <  3:303234/149‑1 (MQ=255)
                                                                                              gCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAAcgcc                                                                                   >  4:250464/1‑113 (MQ=255)
                                                                                              gCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAAcgcc                                                                                   <  3:250464/113‑1 (MQ=255)
                                                                                              gCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCg                                               >  1:376567/1‑149 (MQ=255)
                                                                                                 tCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGttt                                            <  3:304640/149‑1 (MQ=255)
                                                                                                     ccAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTgccg                                        <  4:8693/149‑1 (MQ=255)
                                                                                                      cAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTgccgc                                       <  4:259724/149‑1 (MQ=255)
                                                                                                            ggCCGCGACTGGCGCATCGTGATGGCACCG‑CCGCTGCTCAGCGGACCACAGGCCGACACGCACGCCGAGTGACAGAAGGTCCTGCACGAGGTCAACGCCGCGCGCAAGCTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGccgcccc                                 >  4:202938/1‑149 (MQ=255)
                                                                                                               cgcgACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGccgccccgc                               >  5:181894/1‑148 (MQ=255)
                                                                                                               cgcgACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGccgccccgc                               >  3:394483/1‑148 (MQ=255)
                                                                                                                gcgACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCa                             <  2:376567/149‑1 (MQ=255)
                                                                                                                    cTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACt                           >  4:108197/1‑147 (MQ=255)
                                                                                                                       gcgcATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGAGGCCCGCCGTTTGCCGCCGCCCCGCCACTAAGCt                      >  1:327454/1‑149 (MQ=255)
                                                                                                                        cgcATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATgcgg                                                         <  2:400576/113‑1 (MQ=255)
                                                                                                                        cgcATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTg                     <  4:386432/149‑1 (MQ=255)
                                                                                                                          cATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGa                   <  1:274026/149‑1 (MQ=255)
                                                                                                                           aTCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAg                  <  2:155134/149‑1 (MQ=255)
                                                                                                                               tGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGca                >  1:233761/1‑147 (MQ=255)
                                                                                                                               tGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGca                >  6:216843/1‑147 (MQ=255)
                                                                                                                                   ggCGCGG‑CCGCTGCTCAGCGGCCGCCTGGGCCACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCCCCCCGCCACAAAGCTGGAGCACAGTGc          >  4:141155/1‑149 (MQ=255)
                                                                                                                                    aggcGG‑CCGCTGAGCAGCGGCCGGCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGtt                                             <  7:307256/110‑1 (MQ=255)
                                                                                                                                    aggcGG‑CCGCTGAGCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGtt                                             >  8:307256/4‑113 (MQ=255)
                                                                                                                                      aCGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGCACAGTGCTg        >  1:315703/1‑148 (MQ=255)
                                                                                                                                      aCGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGCACAGTGCTg        <  2:315703/148‑1 (MQ=255)
                                                                                                                                        gg‑CCGCTGAGCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGcaca              <  7:26596/140‑1 (MQ=255)
                                                                                                                                        gg‑CCGCTGAGCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGCACAGTGCTGGcc     >  6:175717/1‑149 (MQ=255)
                                                                                                                                        gg‑CCGCTGAGCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCAGTTTGCCGCCGCCCCGCCACTAAGCTGGAGcaca              >  8:26596/1‑140 (MQ=255)
                                                                                                                                         g‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGCACAGTGCTGGCCg    <  6:54931/149‑1 (MQ=255)
                                                                                                                                            cGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGCACAGTGCTGGCCggg  <  2:233761/149‑1 (MQ=255)
                                                                                                                                            cGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGGGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCAGCCGCCCCTCCACTAAGCTGGAGCACAGTTCTGGCCggg  <  5:40763/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AGCCTGTCGGGGCCACGCGACGCACGCGCCGCCATGCATGCCATCGAGGAAAGCTTCTGCCAGGTGGTGCTCGACCCGCAATTCGTCGATATCGGCGTCAGCCAGGAGGGCCGCGACTGGCGCATCGTGCTGGCACGG‑CCGCTGCTCAGCGGCCGCCTGGGCGACTGGCAGGCCGAGGGACAGAAGGTGCTGCAGGAGGTCAACGCCGCGCGCAAGGTGCCACGGCAATGCGGCGGCCAGCCGTTTGCCGCCGCCCCGCCACTAAGCTGGAGCACAGTGCTGGCCGGG  >  NC_002947/4042177‑4042464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑