Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,057,759 C→T 7.1% V305V (GTG→GTA)  PP_3576 ← transmembrane sensor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,057,7590CT 7.1% 167.7 / 2.0 56V305V (GTG→GTA) PP_3576transmembrane sensor
Reads supporting (aligned to +/- strand):  ref base C (28/24);  new base T (2/2);  total (30/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.39e-01

CGCGCGCCAGTGGGCGCAGGCGGCAAGATTTTACTGCAGGCATTGAACAAGGCTCCCCTTTTATTTTCTCCTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCGGTAACACGCAAGCGCTCCAGTTCTGGTGCCCAGCGCAGTACGC  >  NC_002947/4057619‑4057895
                                                                                                                                            |                                                                                                                                        
cgcgcgcCAGTGGGCGCAGGCGGCAAGATTTTACTGCAGGCATTGAACAAGGCTCCCCTTTTATTTTCTCCTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAAt                                                                                                                                   <  2:320428/148‑1 (MQ=255)
 gcgcgcCAGTGGGCGCAGGCGGCAAGATTTTACTGCAGGCATTGAACAAGGCTCCCCTTTTATTTTCTCCTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATaa                                                                                                                                 <  1:358481/149‑1 (MQ=255)
   gcgcCAGTGGGCGCAGGCGGCAAGATTTTACTGCAGGCATTGAACAAGGCTCCCCTTTTATTTTCTCCTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAAcg                                                                                                                               >  4:328097/1‑149 (MQ=255)
       cAGTGGGCGCAGGCGGCAAGATTTTACTGCAGGCATTGAACAAGGCTCCCCTTTTATTTTCTCCTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCAc                                                                                                                                        >  6:90611/1‑136 (MQ=255)
       cAGTGGGCGCAGGCGGCAAGATTTTACTGCAGGCATTGAACAAGGCTCCCCTTTTATTTTCTCCTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCAc                                                                                                                                        <  5:90611/136‑1 (MQ=255)
         gTGGGCGCAGGCGGCAAGATTTTACTGCAGGCATTGAACAAGGCTCCCCTTTTATTTTCTCCTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCg                                                                                                                         <  5:80835/149‑1 (MQ=255)
                   gcggcAAGATTTTACTGCAGGCATTGAACAAGGCTCCCCTTTTATTTTCTCCTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCGCGTGCGGGCAGGCGCCCCCACTCACGCGTGCGGGTCTGcccc                                                                                                               >  2:168670/1‑149 (MQ=255)
                    cggcAAGATTTTACTGCAGGCATTGAACAAGGCTCCCCTTTTATTTTCTCCTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCt                                                                                                              <  3:31144/149‑1 (MQ=255)
                       cAAGATTTTACTGCAGGCATTGAACAAGGCTCCCCTTTTATTTTCTCCTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGAGTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGGACATGc                                                                                                            >  4:249168/1‑148 (MQ=255)
                        aaGATTTTACTGCAGGCATTGAACAAGGCTCCCCTTTTATTTTCTCCTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCa                                                                                                           >  7:254103/1‑148 (MQ=255)
                            ttttACTGCAGGCATTGAACAAGGCTCCCCTTTTATTTTCTCCTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACgg                                                                                                       <  8:254103/148‑1 (MQ=255)
                                     aGGCATTGAACAAGGCTCCCCTTTTATTTTCTCCTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTggcg                                                                                             >  4:228196/1‑149 (MQ=255)
                                         aTTGAACAAGGCTCCCCTTTTATTTTCTCCTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTggcggc                                                                                           >  7:15789/1‑147 (MQ=255)
                                         aTTGAACAAGGCTCCCCTTTTATTTTCTCCTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTggcggc                                                                                           >  3:79009/1‑147 (MQ=255)
                                         aTTCAACAAGGCTCCCCTTTTATTTTCTCCTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACATGCAACGGCAGGCTGgcggcg                                                                                          >  7:163259/1‑148 (MQ=255)
                                                ccggCGCCCCTTGTATTTGCTCCTCCCGGTACTGACCGGCGCGTGAAAAAACGGGGACAGTCTCATGAGCGATTGTCTCGGGCGGGCAGGCGCCCCCACTACCGCGGGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGc                                                                                  <  1:168670/147‑1 (MQ=255)
                                                             tATTTTCTCCTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAg                                                                                     >  2:9893/1‑133 (MQ=255)
                                                             tATTTTCTCCTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAg                                                                                     <  1:9893/133‑1 (MQ=255)
                                                               ttttCTCCTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCcggtcggt                                                                   <  1:24079/149‑1 (MQ=255)
                                                                   ctcCTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTgtc                                                                <  3:328097/148‑1 (MQ=255)
                                                                     ccTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGc                                                                                                      <  2:283061/108‑1 (MQ=255)
                                                                      cTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTgtcgtc                                                             >  1:81822/1‑148 (MQ=255)
                                                                        aCCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCcggt                                                                       >  3:136832/1‑136 (MQ=255)
                                                                        aCCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCcggt                                                                       <  4:136832/136‑1 (MQ=255)
                                                                            gTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCg                                                       >  7:34637/1‑148 (MQ=255)
                                                                                   ccGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGc                                               <  8:15789/149‑1 (MQ=255)
                                                                                      gCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCgg                                            <  6:148607/149‑1 (MQ=255)
                                                                                       cGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCTGTCGGTGTCGTCAAGGCGGAACGTGCCGGt                                           <  3:315799/149‑1 (MQ=255)
                                                                                           tGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGa                                                                                         <  6:56602/99‑1 (MQ=255)
                                                                                           tGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGa                                                                                         >  5:56602/1‑99 (MQ=255)
                                                                                            gAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGc                                                        >  5:404933/1‑131 (MQ=255)
                                                                                            gAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGc                                                        <  6:404933/131‑1 (MQ=255)
                                                                                              aaaaaaGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCGGTaa                                         <  5:131144/144‑1 (MQ=255)
                                                                                              aaaaaaGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCGGTaa                                         >  6:131144/1‑144 (MQ=255)
                                                                                                      ggACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCGGTAACACGCAAGCGCTc                            >  5:349352/1‑149 (MQ=255)
                                                                                                            tctcATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCGGTAACACGCAAGCGCTCCAGTTc                      >  5:405832/1‑149 (MQ=255)
                                                                                                             ctcATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCGGTAACACGCAAGCGCTCCAGTTCt                     >  6:252728/1‑149 (MQ=255)
                                                                                                              tcATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCGGTAACACGCAAGCGCTCCAGTTCTg                    >  7:3292/1‑149 (MQ=255)
                                                                                                              tcATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCGGTAACACGCAAGCGCTCCAGTTCTg                    >  1:118984/1‑149 (MQ=255)
                                                                                                                aTGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCGGTAACACGCAAGCGCTCCAGTTCTg                    <  2:118984/147‑1 (MQ=255)
                                                                                                                aTGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCGGTAACACGCAAGCGCTCCAGTTCTGGt                  <  3:228196/149‑1 (MQ=255)
                                                                                                                 tGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCGGTAACACGCAAGCGCTcc                           >  5:255501/1‑139 (MQ=255)
                                                                                                                 tGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCGGTAACACGCAAGCGCTcc                           <  6:255501/139‑1 (MQ=255)
                                                                                                                 tGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCGGTAACACGCAAGCGCTCCAGTTCTGGTg                 >  7:304590/1‑149 (MQ=255)
                                                                                                                 tGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCGGTAACACGCAAGCGCTCCAGTTCTGGTg                 <  8:34637/149‑1 (MQ=255)
                                                                                                                   aTCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCGGt                                           <  6:87782/121‑1 (MQ=255)
                                                                                                                   aTCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCGGt                                           >  5:87782/1‑121 (MQ=255)
                                                                                                                   aTCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCGGTAACACGCAAGCGCTCCAGTTCTGGTGcc               >  3:50813/1‑149 (MQ=255)
                                                                                                                     cGATTTTCTCGTGCGGTCAGGCTTACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCcggtc                                                                      >  1:7009/1‑92 (MQ=255)
                                                                                                                     cGATTTTCTCGTGCGGTCAGGCTTACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCcggtc                                                                      >  1:184946/1‑92 (MQ=255)
                                                                                                                     cGATTTTCTCGTGCGGTCAGGCTTACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCcggtc                                                                      <  2:184946/92‑1 (MQ=255)
                                                                                                                     cGATTTTCTCGTGCGGTCAGGCTTACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCcggtc                                                                      <  2:7009/92‑1 (MQ=255)
                                                                                                                      gATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCGGTAac                                        <  4:209960/121‑1 (MQ=255)
                                                                                                                      gATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCGGTAac                                        >  3:209960/1‑121 (MQ=255)
                                                                                                                            ctcGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCGGTAACACGCAAGCGCTCCAGTTCTGGTGCCCAGCGCAg       >  2:55557/1‑148 (MQ=255)
                                                                                                                                tGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCGGTAACACGCAAGCGCTCCAGTTCTGGTGCCCAGCGCAGTACGc  >  5:48417/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                        
CGCGCGCCAGTGGGCGCAGGCGGCAAGATTTTACTGCAGGCATTGAACAAGGCTCCCCTTTTATTTTCTCCTACCGGTAATGACCGGCGAGTGAAAAAAAGGGGACAGTCTCATGATCGATTTTCTCGTGCGGTCAGGCTCACCCAATAACGCGTGCGGGTCTGCACCTGCAACGGCAGGCTGGCGGCGAGCAGGGCCAACACCCGGTCGGTGTCGTCAAGGCGGAACGTGCCGGTAACACGCAAGCGCTCCAGTTCTGGTGCCCAGCGCAGTACGC  >  NC_002947/4057619‑4057895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑