Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,066,001 A→C 7.9% R1021R (CGT→CGG)  mdtC ← multidrug transporter membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,066,0010AC 7.9% 143.6 / 4.4 51R1021R (CGT→CGG) mdtCmultidrug transporter membrane protein
Reads supporting (aligned to +/- strand):  ref base A (24/23);  new base C (2/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01

CACTGAGCAAGGCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCTGGCTCAGCAGC  >  NC_002947/4065857‑4066148
                                                                                                                                                |                                                                                                                                                   
cACTGAGCAAGGCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGt                                                                                                                                                 >  4:189588/1‑149 (MQ=255)
 aCTGAGCAAGGCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGtt                                                                                                                                                >  1:302661/1‑149 (MQ=255)
         aGGCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGt                                                                                                                                        <  5:400112/149‑1 (MQ=255)
          ggCCGCGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGGTGAATCGGTg                                                                                                                                       >  3:382707/1‑149 (MQ=255)
          ggCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTg                                                                                                                                       <  2:302661/149‑1 (MQ=255)
          ggCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTg                                                                                                                                       <  3:53367/149‑1 (MQ=255)
            ccACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGGCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGATACGGTGgc                                                                                                                                     <  2:320111/149‑1 (MQ=255)
                 cacaACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAgg                                                                                                                                <  6:400042/149‑1 (MQ=255)
                    aaCCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTCCAGGGCACGGTGAAGTGGGGGCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCgg                                                                                                                             <  7:143489/149‑1 (MQ=255)
                           cACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCcaggt                                                                                                                      <  7:63045/149‑1 (MQ=255)
                            aCCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCcaggta                                                                                                                     <  2:92652/149‑1 (MQ=255)
                             ccGAGCTGGCGCGCTCCACGGCCACGTGTCAGTGCTTGAAGGCCAGGGTGATGTGGGGGCTGGCCAAATGTCATAGGGGGGTTTCCAGAGCGGCGTGGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCcaggtac                                                                                                                    <  8:131333/149‑1 (MQ=255)
                               gAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGGGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTAGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCcaggtacag                                                                                                                  <  1:309209/149‑1 (MQ=255)
                                    ggCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTaaa                                                                                                             <  5:51211/149‑1 (MQ=255)
                                     gcgcGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAac                                                                                                            >  2:119324/1‑149 (MQ=255)
                                             cacggccacggGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGcg                                                                                                    >  7:112794/1‑149 (MQ=255)
                                              acggccacggGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGcgt                                                                                                   >  7:120258/1‑149 (MQ=255)
                                                     cggGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGcgtcgt                                                                                                <  2:172139/145‑1 (MQ=255)
                                                     cggGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGcgtcgt                                                                                                >  1:172139/1‑145 (MQ=255)
                                                           aGTGATTGCAGGGCACGGTGAAGTGGGGTGTGGGAAAAAGTCATAGCGGGGTTTCCAGAGGGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAATGGGTg                                                                                      <  4:382707/149‑1 (MQ=255)
                                                            gTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAATGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGt                                                                                       >  6:105752/1‑147 (MQ=255)
                                                            gTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGa                                                                                     <  3:380078/149‑1 (MQ=255)
                                                                    aGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTgg                                                                             <  8:120258/149‑1 (MQ=255)
                                                                      ggCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCt                                                                           <  3:271750/149‑1 (MQ=255)
                                                                      ggCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCt                                                                           >  2:134027/1‑149 (MQ=255)
                                                                         aCGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCAAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGaa                                                                        >  4:382581/1‑149 (MQ=255)
                                                                          cGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAac                                                                       <  3:189588/149‑1 (MQ=255)
                                                                           ggTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAaca                                                                      >  3:147657/1‑149 (MQ=255)
                                                                             tGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAacac                                                                     >  7:290187/1‑148 (MQ=255)
                                                                              gAAGTGGGGTCTGGGCAAAAGTCATAGCGCGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAacac                                                                     >  1:365653/1‑147 (MQ=255)
                                                                                      gTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCAcc                                                            >  5:207940/1‑148 (MQ=255)
                                                                                         tGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCgat                                                         >  2:6757/1‑148 (MQ=255)
                                                                                            gCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGatgatg                                                     >  2:311117/1‑149 (MQ=255)
                                                                                            gCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGatgatg                                                     <  6:207940/149‑1 (MQ=255)
                                                                                            gCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGatgatg                                                     >  1:102123/1‑149 (MQ=255)
                                                                                             cAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATgg                                                    >  1:22126/1‑149 (MQ=255)
                                                                                                 aGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAggg                                                                                        <  5:250525/109‑1 (MQ=255)
                                                                                                 aGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAggg                                                                                        >  6:250525/1‑109 (MQ=255)
                                                                                                           gggTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGc                                      >  1:107027/1‑149 (MQ=255)
                                                                                                            ggTGTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCt                                     <  8:184322/149‑1 (MQ=255)
                                                                                                                   agagCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCAt                              >  6:391511/1‑149 (MQ=255)
                                                                                                                          cgTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTgcg                       <  4:147657/149‑1 (MQ=255)
                                                                                                                           gTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTgcgc                      <  2:365653/149‑1 (MQ=255)
                                                                                                                              ggTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCtt                   <  1:167888/149‑1 (MQ=255)
                                                                                                                                      gcctgcgcCACCGTTTCAACCGTTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCTgg           >  7:291059/5‑149 (MQ=255)
                                                                                                                                      gcctgcgcCACCGTTTCAACCGTTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCTgg           >  8:331736/5‑149 (MQ=255)
                                                                                                                                         cgcgCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCTGGCt         >  1:278040/1‑148 (MQ=255)
                                                                                                                                          gcgcCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCTGGCt         >  2:302733/1‑147 (MQ=255)
                                                                                                                                            gcCACCGTTTCAACCGTTGGCGCGGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCTGGCTcagc     <  5:129947/149‑1 (MQ=255)
                                                                                                                                            gcCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCTGGCTcagc     >  8:5642/1‑149 (MQ=255)
                                                                                                                                             ccACCGTTTCAACCGTTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCTGGCTcagca    <  7:331736/149‑1 (MQ=255)
                                                                                                                                                aCGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCTGGCTCagcagc  >  3:93512/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CACTGAGCAAGGCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCTGGCTCAGCAGC  >  NC_002947/4065857‑4066148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑