Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,066,013 G→T 7.5% H1017Q (CAC→CAA mdtC ← multidrug transporter membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,066,0130GT 7.5% 166.7 / 3.4 53H1017Q (CAC→CAAmdtCmultidrug transporter membrane protein
Reads supporting (aligned to +/- strand):  ref base G (24/25);  new base T (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

AGGCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCTGGCTCAGCAGCAACGGCAATGC  >  NC_002947/4065866‑4066159
                                                                                                                                                   |                                                                                                                                                  
aGGCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGt                                                                                                                                                   <  5:400112/149‑1 (MQ=255)
 ggCCGCGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGGTGAATCGGTg                                                                                                                                                  >  3:382707/1‑149 (MQ=255)
 ggCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTg                                                                                                                                                  <  3:53367/149‑1 (MQ=255)
 ggCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTg                                                                                                                                                  <  2:302661/149‑1 (MQ=255)
   ccACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGGCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGATACGGTGgc                                                                                                                                                <  2:320111/149‑1 (MQ=255)
        cacaACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAgg                                                                                                                                           <  6:400042/149‑1 (MQ=255)
           aaCCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTCCAGGGCACGGTGAAGTGGGGGCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCgg                                                                                                                                        <  7:143489/149‑1 (MQ=255)
                  cACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCcaggt                                                                                                                                 <  7:63045/149‑1 (MQ=255)
                   aCCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCcaggta                                                                                                                                <  2:92652/149‑1 (MQ=255)
                    ccGAGCTGGCGCGCTCCACGGCCACGTGTCAGTGCTTGAAGGCCAGGGTGATGTGGGGGCTGGCCAAATGTCATAGGGGGGTTTCCAGAGCGGCGTGGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCcaggtac                                                                                                                               <  8:131333/149‑1 (MQ=255)
                      gAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGGGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTAGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCcaggtacag                                                                                                                             <  1:309209/149‑1 (MQ=255)
                           ggCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTaaa                                                                                                                        <  5:51211/149‑1 (MQ=255)
                            gcgcGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAac                                                                                                                       >  2:119324/1‑149 (MQ=255)
                                    cacggccacggGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGcg                                                                                                               >  7:112794/1‑149 (MQ=255)
                                     acggccacggGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGcgt                                                                                                              >  7:120258/1‑149 (MQ=255)
                                            cggGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGcgtcgt                                                                                                           <  2:172139/145‑1 (MQ=255)
                                            cggGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGcgtcgt                                                                                                           >  1:172139/1‑145 (MQ=255)
                                                  aGTGATTGCAGGGCACGGTGAAGTGGGGTGTGGGAAAAAGTCATAGCGGGGTTTCCAGAGGGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAATGGGTg                                                                                                 <  4:382707/149‑1 (MQ=255)
                                                   gTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAATGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGt                                                                                                  >  6:105752/1‑147 (MQ=255)
                                                   gTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGa                                                                                                <  3:380078/149‑1 (MQ=255)
                                                           aGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTgg                                                                                        <  8:120258/149‑1 (MQ=255)
                                                             ggCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCt                                                                                      <  3:271750/149‑1 (MQ=255)
                                                             ggCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCt                                                                                      >  2:134027/1‑149 (MQ=255)
                                                                aCGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCAAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGaa                                                                                   >  4:382581/1‑149 (MQ=255)
                                                                 cGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAac                                                                                  <  3:189588/149‑1 (MQ=255)
                                                                  ggTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAaca                                                                                 >  3:147657/1‑149 (MQ=255)
                                                                    tGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAacac                                                                                >  7:290187/1‑148 (MQ=255)
                                                                     gAAGTGGGGTCTGGGCAAAAGTCATAGCGCGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAacac                                                                                >  1:365653/1‑147 (MQ=255)
                                                                             gTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCAcc                                                                       >  5:207940/1‑148 (MQ=255)
                                                                                tGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCgat                                                                    >  2:6757/1‑148 (MQ=255)
                                                                                   gCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGatgatg                                                                <  6:207940/149‑1 (MQ=255)
                                                                                   gCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGatgatg                                                                >  1:102123/1‑149 (MQ=255)
                                                                                   gCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGatgatg                                                                >  2:311117/1‑149 (MQ=255)
                                                                                    cAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATgg                                                               >  1:22126/1‑149 (MQ=255)
                                                                                        aGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAggg                                                                                                   <  5:250525/109‑1 (MQ=255)
                                                                                        aGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAggg                                                                                                   >  6:250525/1‑109 (MQ=255)
                                                                                                  gggTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGc                                                 >  1:107027/1‑149 (MQ=255)
                                                                                                   ggTGTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCt                                                <  8:184322/149‑1 (MQ=255)
                                                                                                          agagCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCAt                                         >  6:391511/1‑149 (MQ=255)
                                                                                                                 cgTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTgcg                                  <  4:147657/149‑1 (MQ=255)
                                                                                                                  gTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTgcgc                                 <  2:365653/149‑1 (MQ=255)
                                                                                                                     ggTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCtt                              <  1:167888/149‑1 (MQ=255)
                                                                                                                             gcctgcgcCACCGTTTCAACCGTTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCTgg                      >  7:291059/5‑149 (MQ=255)
                                                                                                                             gcctgcgcCACCGTTTCAACCGTTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCTgg                      >  8:331736/5‑149 (MQ=255)
                                                                                                                                cgcgCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCTGGCt                    >  1:278040/1‑148 (MQ=255)
                                                                                                                                 gcgcCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCTGGCt                    >  2:302733/1‑147 (MQ=255)
                                                                                                                                   gcCACCGTTTCAACCGTTGGCGCGGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCTGGCTcagc                <  5:129947/149‑1 (MQ=255)
                                                                                                                                   gcCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCTGGCTcagc                >  8:5642/1‑149 (MQ=255)
                                                                                                                                    ccACCGTTTCAACCGTTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCTGGCTcagca               <  7:331736/149‑1 (MQ=255)
                                                                                                                                       aCGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCTGGCTCagcagc             >  3:93512/1‑148 (MQ=255)
                                                                                                                                               aaCGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCt                        >  6:322675/1‑129 (MQ=255)
                                                                                                                                               aaCGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCt                        <  5:322675/129‑1 (MQ=255)
                                                                                                                                                 cGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCTGGCTCAGCAGCAACGGCAATgc  <  2:22126/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGGCCACGCACAACCCGGCACCGAGCAGGCGCGAGCCACGGCCACGGGTCAGTGCTTGCAGGGCACGGTGAAGTGGGGTCTGGGCAAAAGTCATAGCGGGGTTTCCAGAGCGGCGTCGGTACGCACGCCGCGCCAACGGTTGAAACGGTGGCGCAGGCGGTCCAGGTACAGGTAAACCACCGGCGTCGTATAAAGGGTGAGGATCTGGCTGAACACCAGGCCACCGATGATGGTCAGGCCCAGCGGCTGACGCATTTCTGCGCCTTCAGCCTGGCTCAGCAGCAACGGCAATGC  >  NC_002947/4065866‑4066159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: