Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,143,612	T→G	7.4%	F84C (TTC→TGC)	PP_3647 →	oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,143,612	0	T	G	7.4%	206.0 / 7.1	68	F84C (TTC→TGC)	PP_3647	oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (33/30);  new base G (3/2);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

CCAGTGCATTCAGCGCGCTGTCGATCGACCCGGCGCTGGTGCTGTTCTGCCCCAACTACGCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAGTACGACGGCGGTGACCACGCGATCATTGTCGGCGCG  >  NC_002947/4143465‑4143760                                                                                                                                                    |                                                                                                                                                     ccAGTGCATTCAGCGCGCTGTCGATCGACCCGGCGCTGGTGCTGTTCTGCCCCAACTACGCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTc                                                                                                                                                     >  5:49639/1‑149 (MQ=255) ccAGTGCATTCAGCGCGCTGTCGATCGACCCGGCGCTGGTGCTGTTCTGCCCCAACTACGCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTc                                                                                                                                                     <  8:146000/149‑1 (MQ=255)       cATTCAGCGCGCTGTCGATCGACCCGGCGCTGGTGCTGTTCTGCCCCAACTACGCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAgg                                                                                                                                                <  6:284330/148‑1 (MQ=255)         ttCAGCGCGCTGTCGATCGACCCGGCGCTGGTGCTGTTCTGCCCCAACTACGCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAggcaa                                                                                                                                             <  8:105607/149‑1 (MQ=255)             gcgcgcTGTCGATCGACCCGGCGCTGGTGCTGTTCTGCCCCAACTACGCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAggcaaggg                                                                                                                                          >  8:163966/1‑148 (MQ=255)                 gcTGTCGATCGACCCGGCGCTGGTGCTGTTCTGCCCCAACTACGCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAggcaagggcaag                                                                                                                                      >  6:5169/1‑148 (MQ=255)                     tcgatcgaCCCGGCGCTGGTGCTGTTCTGCCCCAACTACGCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAg                                                                                                                                                 <  8:137895/133‑1 (MQ=255)                     tcgatcgaCCCGGCGCTGGTGCTGTTCTGCCCCAACTACGCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAg                                                                                                                                                 >  7:137895/1‑133 (MQ=255)                     tcgatcgaCCCGGCGCTGGTGCTGTTCTGCCCCAACTACGCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGAc                                                                                                                                   <  6:153693/147‑1 (MQ=255)                     tcgatcgaCCCGGCGCTGGTGCTGTTCTGCCCCAACTACGCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACaa                                                                                                                                 <  4:270775/149‑1 (MQ=255)                            aCCCGGCGCTGGTGCTGTTCTGCCCCAACTACGCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAg                                                                                                                          <  5:189436/149‑1 (MQ=255)                            aCCCGGCGCTGGTGCTGTTCTGCCCCAACTACGCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAg                                                                                                                          >  7:8032/1‑149 (MQ=255)                             cccGGCGCTGGTGCTGTTCTGCCCCAACTACGCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAgg                                                                                                                         <  5:375588/149‑1 (MQ=255)                               cGGCGCTGGTGCTGTTCTGCCCCAACTACGCCTCCGACACCTACCCTATCCTGCGCTACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAATGCCAAGGGc                                                                                                                       <  5:191852/149‑1 (MQ=255)                                  cgcTGGTGCTGTTCTGCCCCAACTACGCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTc                                                                                                                    <  6:179804/149‑1 (MQ=255)                                       gTGCTGTTCTGCCCCAACTACGCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGt                                                                                                                <  7:163966/148‑1 (MQ=255)                                         gCTGTTCTGCCCCAACTACGCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGa                                                                                                                                    <  1:256251/126‑1 (MQ=255)                                         gCTGTTCTGCCCCAACTACGCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCAGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGa                                                                                                                                    >  2:256251/1‑126 (MQ=255)                                               cTGCCCCAACTACGCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTg                                                                                                        <  5:374143/148‑1 (MQ=255)                                                  ccccAACTACGCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTgagc                                                                                                     <  8:8032/148‑1 (MQ=255)                                                   cccAACTACGCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTgagc                                                                                                     >  1:229/1‑147 (MQ=255)                                                            gCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCaa                                                                                          <  7:370360/149‑1 (MQ=255)                                                              cTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAAcc                                                                                        >  3:54314/1‑149 (MQ=255)                                                              cTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAAcc                                                                                        >  4:232783/1‑149 (MQ=255)                                                               tCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAAccc                                                                                       >  8:4838/1‑149 (MQ=255)                                                               tCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAAccc                                                                                       >  4:216677/1‑149 (MQ=255)                                                                        tACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGc                                                                              >  7:358264/1‑149 (MQ=255)                                                                           ccGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGcc                                                                             >  3:73469/1‑147 (MQ=255)                                                                            cGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAg                                                                          <  6:67840/149‑1 (MQ=255)                                                                             gATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAgg                                                                         >  2:198225/1‑149 (MQ=255)                                                                                   gcgcGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACgg                                                                   >  1:187622/1‑149 (MQ=255)                                                                                     gcgACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGc                                                                  >  2:49438/1‑148 (MQ=255)                                                                                      cgACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGa                                                                >  4:174904/1‑149 (MQ=255)                                                                                           gcaagcaGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGatcatc                                                           <  8:98745/149‑1 (MQ=255)                                                                                             aagcaGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGatcatc                                                           >  4:297416/1‑147 (MQ=255)                                                                                                caGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTg                                                      <  3:216677/149‑1 (MQ=255)                                                                                                   ttCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCg                                                    >  2:76132/1‑148 (MQ=255)                                                                                                   ttCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCg                                                    >  3:162544/1‑148 (MQ=255)                                                                                                      gCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAg                                                                          >  5:306388/1‑123 (MQ=255)                                                                                                       ccATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCATTCGCAGGCAAGGGAAAGGACAAGGCCAAGGGCGTCGAGTGGCCCCTGAGCGCGCTGGGAACCCCCCTGCTGGCCAAGGCCACGGCGATCAGCGAGGGCGAATtg                                               >  6:240976/1‑149 (MQ=255)                                                                                                         aTCCCCCTGCTGGCCCCCGTACATCCCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATtgtg                                             <  5:240976/149‑1 (MQ=255)                                                                                                         aTCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATtgtg                                             <  7:4838/149‑1 (MQ=255)                                                                                                         aTCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATtgtg                                             <  1:76132/149‑1 (MQ=255)                                                                                                           ccACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATtgtg                                             >  1:348337/1‑147 (MQ=255)                                                                                                            cACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGgc                                           <  1:49438/148‑1 (MQ=255)                                                                                                             aCCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATtgt                                              <  4:251645/144‑1 (MQ=255)                                                                                                             aCCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATtgt                                              >  3:251645/1‑144 (MQ=255)                                                                                                             aCCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGgcgc                                         >  4:324455/1‑149 (MQ=255)                                                                                                               ctgctgTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGa                                       <  6:49639/149‑1 (MQ=255)                                                                                                                tgctgTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCTACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAg                                      >  1:331653/1‑149 (MQ=255)                                                                                                                              cAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAGTACGACGGCGGTGa                        <  3:324455/149‑1 (MQ=255)                                                                                                                               aGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAGTACGACGGCGGTGAc                       <  5:5169/149‑1 (MQ=255)                                                                                                                               aGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAGTACGACGGCGGTGAc                       >  2:179101/1‑149 (MQ=255)                                                                                                                               aGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAGTACGACGGCGGTGAc                       <  3:174904/149‑1 (MQ=255)                                                                                                                                       gAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAGTACGACGGCGGTGACCACGCGAt               >  8:204228/1‑149 (MQ=255)                                                                                                                                        ttgcccttGCCTGCGAAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAGTACGACGGCGGTGACCACGCGAt               >  2:107600/8‑148 (MQ=255)                                                                                                                                          gCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCCACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAGTACGACGGCGGTGACCACGCGATCAt            >  2:339999/1‑149 (MQ=255)                                                                                                                                           cccttGCCTGCGAAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAGTacga                                 >  6:338948/5‑127 (MQ=255)                                                                                                                                           cccttGCCTGCGAAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAGTacga                                 <  5:338948/123‑1 (MQ=255)                                                                                                                                           cGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAGTACGACGGCGGTGACCACGCGATCAtt           <  8:358264/149‑1 (MQ=255)                                                                                                                                           cGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAGTACGACGGCGGTGACCACGCGATCAtt           <  3:232783/149‑1 (MQ=255)                                                                                                                                            gTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAGTACGACGGCGGTGACCACGCGATCATTg          >  2:394524/1‑149 (MQ=255)                                                                                                                                             cttGCCTGCGAAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAGTACGACGGCGGTGACCACGCGATCATTGt         >  8:386894/3‑149 (MQ=255)                                                                                                                                                gCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAGTACGACGGCGGTGACCACGCGa                <  4:69002/139‑1 (MQ=255)                                                                                                                                                gCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAGTACGACGGCGGTGACCACGCGa                >  3:69002/1‑139 (MQ=255)                                                                                                                                                gCCTGCGAAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAGTACGACGGCGGTGACCACGCGATCATTGTCg       <  1:107600/148‑1 (MQ=255)                                                                                                                                                  cTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAGTACGACGGCGGTGACCACGCGATCATTGTCGgcg    >  2:53508/1‑149 (MQ=255)                                                                                                                                                  cTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAGTACGACGGCGGTGACCACGCGATCATTGTCGgcg    >  8:84053/1‑149 (MQ=255)                                                                                                                                                   ttCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAGTACGACGGCGGTGACCACGCGATCATTGTCGgcgc   >  3:99718/1‑149 (MQ=255)                                                                                                                                                    tCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAGTACGACGGCGGTGACCACGCGATCATTGTCGGcgcg  >  2:375841/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CCAGTGCATTCAGCGCGCTGTCGATCGACCCGGCGCTGGTGCTGTTCTGCCCCAACTACGCCTCCGACACCTACCCGATCCTGCGCGACAGCAAGCAGTTCGCCATCCACCTGCTGTCCGCCGACCAGACCGCCGAGGCGTATGCCTTCGCAGGCAAGGGCAAGGACAAGGCCAAGGGCGTCGAGTGGCACCTGAGCGAGCTGGGCAACCCCCTGCTGGCCAAGGCCACGGCGATCATCGAGTGCGAATTGTGGCGCGAGTACGACGGCGGTGACCACGCGATCATTGTCGGCGCG  >  NC_002947/4143465‑4143760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑