Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	313,967	C→G	7.2%	V192L (GTG→CTG) ‡	yrfG ←	purine nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	313,967	0	C	G	7.2%	265.2 / 8.8	83	V192L (GTG→CTG) ‡	yrfG	purine nucleotidase
Reads supporting (aligned to +/- strand):  ref base C (40/37);  new base G (3/3);  total (43/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.65e-01

CCTGACTGATCGCTGAAGTCTGTACAGGCCTCTTCGCGGGCGCGCCCGCGAATGGGGCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCATCCCAAAACTGCGGGCTTTCCTTCGCGTAGCC  >  NC_002947/313819‑314108                                                                                                                                                      |                                                                                                                                              ccTGACTGATCGCTGAAGTCTGTACAGGCCTCTTCGCGGGCGCGCCCGCGAATGGGGCCTTACAGGCCTACAAGTAACTACCGGTAATCATCGACAGCCGCAAACTATTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCTGCTGGCGAAc                                                                                                                                               >  5:403046/1‑149 (MQ=255)  ctgactgaTCGCTGAAGTCTGTACAGGCCTCTTCGCGGGCGCGCCCGCGAATGGGGCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCAcc                                                                                                                                              <  7:183686/149‑1 (MQ=255)     actgaTCGCTGAAGTCTGTACAGGCCTCTTCGCGGGCGCGCCCGCGAATGGGGCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGc                                                                                                                                            <  2:245166/148‑1 (MQ=255)         aTCGCTGAAGTCTGTACAGGCCTCTTCGCGGGCGCGCCCGCGAATGGGGCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGGTGCGCCAGc                                                                                                                                        <  7:249042/148‑1 (MQ=255)           cGCTGAAGTCTGTACAGGCCTCTTCGCGGGCGCGCCCGCGAATGGGGCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAg                                                                                                                                     >  2:115679/1‑149 (MQ=255)            gCTGAAGTCTGTACAGGCCTCTTCGCGGGCGCGCCCGCGAATGGGGCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGt                                                                                                                                    >  2:167855/1‑149 (MQ=255)                aaGTCTGTCGAGGCCGGTTCGCGGGGGCGCCCGCGAATGTGGCCTTACAGGCATACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCTGCCCTGCTTTGCTAT‑CCGGCTGGCGGTGCGCCAGccggata                                                                                                                                  <  6:34062/147‑7 (MQ=255)                aaGTCTGTACAGGCCTCTTCGCGGGCGCGCCCGCGAATGGGGCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTTT‑CCGGCTGGCGCACCGCCAGCAAGTGc                                                                                                                                  <  1:16934/147‑1 (MQ=255)                          aGGCCTCTTCGCGGGCGCGCCCGCGAATGGGGCCTTACAGGCCTACCAGTAACTCCCGGTATTCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAAc                                                                                                                      >  5:397064/1‑149 (MQ=255)                            gCCTCTTCGCGGGCGCGCCCGCGAATGGGGCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAAccc                                                                                                                    >  7:153575/1‑148 (MQ=255)                             cccattCGCGGGCGCGCCCGCGAATGGGGCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAAccgc                                                                                                                   >  8:228442/5‑149 (MQ=255)                               tctTCGCGGGCGCGCCCGCGAATGGGGCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAAccgccg                                                                                                                 >  3:37904/1‑149 (MQ=255)                                       ggCGCGCCCGCGAATGGGGCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTgc                                                                                                         <  7:122322/149‑1 (MQ=255)                                        gcgcgcCCGCGAATGGGGCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTgcg                                                                                                        <  7:114866/149‑1 (MQ=255)                                            gcCCGCGAATGGGGCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGa                                                                                                    <  3:34456/149‑1 (MQ=255)                                              ccGCGAATGGGGCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCa                                                                                                      >  2:366235/1‑145 (MQ=255)                                              ccGCGAATGGGGCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCa                                                                                                      <  1:366235/145‑1 (MQ=255)                                              ccGCGAATGGGGCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAAt                                                                                                  >  4:289859/1‑149 (MQ=255)                                              ccGCGAATGGGGCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAAt                                                                                                  >  1:152636/1‑149 (MQ=255)                                               cgcgAATGGGGCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATc                                                                                                 >  1:393261/1‑149 (MQ=255)                                                 cgAATGGGGCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGc                                                                                               <  2:213782/149‑1 (MQ=255)                                                     tGGGGCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTATCCCCGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAg                                                                                            <  1:115679/149‑1 (MQ=255)                                                     tGGGGCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGc                                                                                           >  3:350996/1‑149 (MQ=255)                                                       gggCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCgt                                                                                         <  2:152636/149‑1 (MQ=255)                                                        ggCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTg                                                                                                               <  4:219664/126‑1 (MQ=255)                                                        ggCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTg                                                                                                               >  3:219664/1‑126 (MQ=255)                                                        ggCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCgtg                                                                                        >  4:239095/1‑149 (MQ=255)                                                         gCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCgtgt                                                                                       >  2:276837/1‑149 (MQ=255)                                                              aCAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCa                                                                                                                         >  7:352778/1‑110 (MQ=255)                                                              aCAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCa                                                                                                                         <  8:352778/110‑1 (MQ=255)                                                                aGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGAt                                                                                >  2:346004/1‑149 (MQ=255)                                                                aGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGAt                                                                                <  7:191773/149‑1 (MQ=255)                                                                    cTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATc                                                                                                 <  4:126920/128‑1 (MQ=255)                                                                    cTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATc                                                                                                 >  3:126920/1‑128 (MQ=255)                                                                    cTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAAc                                                                            <  3:239095/149‑1 (MQ=255)                                                                    cTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACAGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAAc                                                                            <  6:403046/149‑1 (MQ=255)                                                                     tACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGaa                                                                             >  1:307149/1‑147 (MQ=255)                                                                          gTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCGCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCTTCGATGAACAGGCTg                                                                      >  7:192908/1‑149 (MQ=255)                                                                          gTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCt                                                                       <  5:380990/148‑1 (MQ=255)                                                                          gTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCt                                                                       >  6:380990/1‑148 (MQ=255)                                                                           tAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGc                                                                     <  2:393261/149‑1 (MQ=255)                                                                               tCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGATTCGCCAGCGTGTCATCGATGAACAGGCTGCGTTc                                                                 >  7:244313/1‑149 (MQ=255)                                                                               tCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTc                                                                 >  5:55482/1‑149 (MQ=255)                                                                                 ccGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATg                                                                               <  1:192351/133‑1 (MQ=255)                                                                                 ccGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATg                                                                               >  2:192351/1‑133 (MQ=255)                                                                                 ccGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCg                                                               <  1:79845/149‑1 (MQ=255)                                                                                 ccGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCg                                                               >  3:312994/1‑149 (MQ=255)                                                                                 ccGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCg                                                               <  8:153575/149‑1 (MQ=255)                                                                                     tAATCCTCGACCGCCGCAAACTCTTCAGTATGCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTc                                                           <  2:307149/149‑1 (MQ=255)                                                                                      aaTCCTCGACCGCCGCAAACTCTTCAGGATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTc                                                           >  1:13102/1‑148 (MQ=255)                                                                                       aTCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCg                                                          <  5:76041/148‑1 (MQ=255)                                                                                             gACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGcc                                                     <  2:26948/147‑1 (MQ=255)                                                                                                    gcAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGc                                            <  6:55482/149‑1 (MQ=255)                                                                                                    gcAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGc                                            <  4:37904/149‑1 (MQ=255)                                                                                                        aCTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTg                                         >  8:325417/1‑148 (MQ=255)                                                                                                          tctTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTc                                                                 >  4:251154/1‑122 (MQ=255)                                                                                                          tctTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTc                                                                 <  3:251154/122‑1 (MQ=255)                                                                                                          tctTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAg                                      >  3:72576/1‑149 (MQ=255)                                                                                                            ttCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAggg                                    >  1:220668/1‑149 (MQ=255)                                                                                                            ttCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAggg                                    >  3:32366/1‑149 (MQ=255)                                                                                                              cAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAgg                                     <  7:228442/146‑1 (MQ=255)                                                                                                              cAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCa                                  >  7:226755/1‑149 (MQ=255)                                                                                                               aGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCAt                                 <  2:13102/149‑1 (MQ=255)                                                                                                                gTAGCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCTCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCATc                                <  8:171959/149‑1 (MQ=255)                                                                                                                 tATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCATcc                               <  3:289859/149‑1 (MQ=255)                                                                                                                 tATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCATcc                               <  4:153312/149‑1 (MQ=255)                                                                                                                 tATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCCCCAGAAAGTGCCCCACACCAAACAGCCGTGCACTGAGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTTCGTTCAGGTTCGAAGCCGATATCCGCCTGCAGGGCATcc                               >  6:10047/1‑149 (MQ=255)                                                                                                                    cccGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCATCCCa                             >  2:154170/1‑148 (MQ=255)                                                                                                                      cgcgGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCATCCCaaaa                          <  4:32366/149‑1 (MQ=255)                                                                                                                           cccTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCATCCCAAAACTGCg                     >  5:335764/1‑149 (MQ=255)                                                                                                                           cccTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCATCCCAAAACTGCg                     <  4:350996/149‑1 (MQ=255)                                                                                                                                   tGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCATCCCAAAACTGCGGGCTTTc              <  1:346004/148‑1 (MQ=255)                                                                                                                                    gCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCACCACACCAAACAGCCGAGAACTGCGCACAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGACGATATCCGCCTGCAGGGCATCCCAAAACTGCGGGCTTTCCt            >  3:295711/1‑149 (MQ=255)                                                                                                                                        t‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCATCCCAAAACTGCGGGCTTTCCTTcgc        >  7:125925/1‑149 (MQ=255)                                                                                                                                        t‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCATCCCAAAACTGCGGGCTTTCCTTcgc        >  3:371472/1‑149 (MQ=255)                                                                                                                                          cttgCTGGCGGTGCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCATCCCAAAACTGCGGGCTTTCCTTcgcg       >  8:245298/4‑149 (MQ=255)                                                                                                                                           ttgCTGGCGGTGCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCATCCCAAAACTGCGGGCTTTc              <  1:241491/139‑1 (MQ=255)                                                                                                                                           ttgCTGGCGGTGCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCATCCCAAAACTGCGGGCTTTc              >  2:241491/3‑141 (MQ=255)                                                                                                                                           ttgCTGGCGGTGCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCATCCCAAAACTGCGGGCTTTCCTTCGCGt      >  1:371713/3‑149 (MQ=255)                                                                                                                                             gCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCATCCCAAAACTGCGGGCTTTCCTTCGCGTAg    >  1:200243/1‑149 (MQ=255)                                                                                                                                                 gcgcACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCATCCCAAAACTGCGGGCTTTCCTTCGCGTAGcc  <  6:397064/147‑1 (MQ=255)                                                                                                                                                   gcACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCATCCCAAAACTGCGGGCTTTCCTTCGCGt      <  5:237379/141‑1 (MQ=255)                                                                                                                                                   gcACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCATCCCAAAACTGCGGGCTTTCCTTCGCGt      >  6:237379/1‑141 (MQ=255)                                                                                                                                                    cACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCATCCCAAAACTGCGGGCTTTCCTTc          <  5:23062/136‑1 (MQ=255)                                                                                                                                                    cACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCATCCCAAAACTGCGGGCTTTCCTTc          >  6:23062/1‑136 (MQ=255)                                                                                                                                                      |                                                                                                                                              CCTGACTGATCGCTGAAGTCTGTACAGGCCTCTTCGCGGGCGCGCCCGCGAATGGGGCCTTACAGGCCTACCAGTAACTCCCGGTAATCCTCGACCGCCGCAAACTCTTCAGTATCCCGCGGCCCTGCTTTGCTAT‑CCGGCTGGCGCACCGCCAGCAAGTGCCCCACACCAAACCGCCGTGCACTGCGCAGAATCGCCAGCGTGTCATCGATGAACAGGCTGCGTTCCGGTTCGAAGCCGATATCCGCCTGCAGGGCATCCCAAAACTGCGGGCTTTCCTTCGCGTAGCC  >  NC_002947/313819‑314108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑