Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,224,495 G→A 7.8% intergenic (‑607/+214) PP_3703 ← / ← PP_3704 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,224,4950GA 7.8% 144.6 / 2.2 51intergenic (‑607/+214)PP_3703/PP_3704hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (18/29);  new base A (2/2);  total (20/31)
Fisher's exact test for biased strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.39e-01

GATCGTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCGCCCATCTGTAGCGGAGATGATACAGAACCTTAGGATTGTCCATGTAAACCTGGAAAAGCGCCAAGGAAGGTTCTGT  >  NC_002947/4224349‑4224638
                                                                                                                                                  |                                                                                                                                               
gATCGTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTg                                                                                                                                               <  5:216689/149‑1 (MQ=255)
  tcgtcgCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCa                                                                                                                                             <  1:81978/149‑1 (MQ=255)
    gtcgCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCAc                                                                                                                                            <  7:94106/148‑1 (MQ=255)
       gCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCtgt                                                                                                                                        <  6:243066/149‑1 (MQ=255)
       gCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATCCGGGAAAACCACGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACAtgt                                                                                                                                        >  2:170849/1‑149 (MQ=255)
        cccTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAATGCAcaggtg                                                                                                                                       >  4:295770/1‑144 (MQ=255)
               aaTCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAATGCACAGGTGCACTGCt                                                                                                                                <  3:295770/149‑1 (MQ=255)
                aTCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTg                                                                                                                               <  6:372340/149‑1 (MQ=255)
                 tCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTgg                                                                                                                              <  2:332690/149‑1 (MQ=255)
                   cAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGtt                                                                                                                            <  6:253476/149‑1 (MQ=255)
                   cAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGtt                                                                                                                            <  7:350249/149‑1 (MQ=255)
                   cAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGtt                                                                                                                            <  3:57448/149‑1 (MQ=255)
                    aaaCGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTg                                                                                                                               >  4:311754/1‑145 (MQ=255)
                    aaaCGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTg                                                                                                                               <  3:311754/145‑1 (MQ=255)
                       cGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCATATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCa                                                                                                                        <  3:272667/149‑1 (MQ=255)
                       cGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGAAACAAAGCCGCCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAACCCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCa                                                                                                                        <  1:177311/149‑1 (MQ=255)
                           aaTGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACgt                                                                                                                     <  1:273783/148‑1 (MQ=255)
                             tGTCCCTAAGCCTGGAGGAGGGGAACAAACTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAACACCCCGCGGAAAATAAACCTAGGTGGCCAAGCAGTGCACCTGTCCATTGCTGGTTGCCACgtgt                                                                                                                   >  8:66079/1‑148 (MQ=255)
                                 ccTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGCCGGCCAGCTACGTGATAAGCGGAGCGATGCGGGAACACGCCGCGGAAAATAAACCTAGGTGGCACAGCAGTGCACCTGTGCATTGCTGGATGCCACGTGTAAAgg                                                                                                              >  2:91167/1‑149 (MQ=255)
                                 ccTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCAc                                                                                                                       >  3:221920/1‑140 (MQ=255)
                                 ccTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCAc                                                                                                                       <  4:221920/140‑1 (MQ=255)
                                       ccTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTAt                                                                                                         >  3:340055/1‑148 (MQ=255)
                                           gcggcgGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAg                                                                                                      <  4:340055/147‑1 (MQ=255)
                                           gcggcgGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGc                                                                                                     <  5:130939/148‑1 (MQ=255)
                                              gcggGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTaaa                                                                                                 <  6:352829/149‑1 (MQ=255)
                                              gcggGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTaaa                                                                                                 <  6:183547/149‑1 (MQ=255)
                                                ggggAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGa                                                                                               >  3:341011/1‑149 (MQ=255)
                                                ggggAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGATAAAGa                                                                                               >  3:335243/1‑149 (MQ=255)
                                                  ggAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACa                                                                                             <  7:332941/149‑1 (MQ=255)
                                                   gAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCt                                                                                                                                >  8:111149/1‑113 (MQ=255)
                                                   gAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCt                                                                                                                                <  7:111149/113‑1 (MQ=255)
                                                      caacCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGAc                                                                                         <  1:123192/149‑1 (MQ=255)
                                                         agtCGCTCCCGTAACATAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAAGCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTTAAGACAGGACTgg                                                                                      <  1:170849/147‑1 (MQ=255)
                                                          cTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTgg                                                                                      >  8:219613/1‑148 (MQ=255)
                                                          cTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTgg                                                                                      <  7:219613/148‑1 (MQ=255)
                                                                  cGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCgcgc                                                                             >  1:318651/1‑149 (MQ=255)
                                                                    tAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCGccc                                                                           <  1:366397/149‑1 (MQ=255)
                                                                    tAACAAAGACGGCCAGATACGTGATAAGAGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCGGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATAGCGcc                                                                            >  7:111636/1‑148 (MQ=255)
                                                                       cAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCGCCCATc                                                                        >  6:168246/1‑149 (MQ=255)
                                                                                  aGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCGCCCATCTGTAGCg                                                                 >  2:72264/1‑145 (MQ=255)
                                                                                      aCGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCTCCCATCTGTAGCGGAgatga                                                          >  2:387020/1‑148 (MQ=255)
                                                                                                cGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCa                                                                                                                        >  3:324824/1‑76 (MQ=255)
                                                                                                cGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCa                                                                                                                        <  4:324824/76‑1 (MQ=255)
                                                                                                                         gAAAATAAACCTAGGTGGCCCAGAAGTGCTCCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCGCCCATCTGTAGGGGAGATGATACAGAACCTTAGGATTGTCCATGTAAACCTGGaaa                      <  7:66079/149‑1 (MQ=255)
                                                                                                                             aTAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCGCCCATCTGTAGCGGAGATGATACa                                                      <  7:334654/113‑1 (MQ=255)
                                                                                                                             aTAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCGCCCATCTGTAGCGGAGATGATACa                                                      >  8:334654/1‑113 (MQ=255)
                                                                                                                                    tAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGATTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCGCCCATCTGTAGCGGAGATGATACAGAACCTTAGGATTGTCCATGTAAACCTGGAAAAGCGCCaagg            >  2:350210/1‑148 (MQ=255)
                                                                                                                                      ggTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCGCCCATCTGTAGCGGAGATGATACAGAACCTTAGGATTGTCCATGTAAACCTGGAAAAGCGCCaagga           <  4:341011/147‑1 (MQ=255)
                                                                                                                                      ggTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGATTATGAGCTAAAGACAGGACTGGGTATCGCGCCCATCTGTAGCGGAGATGATACAGAACCTTAGGATTGTCCATGTAAACCTGGAAAAGCGCCaagga           <  4:335243/147‑1 (MQ=255)
                                                                                                                                            ccAGCAATGCACAGGTGCACTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGTTATCGCGCCCATCTGTAGCGGAGATGATACa                                                      <  1:360324/98‑1 (MQ=38)
                                                                                                                                            ccAGCAATGCACAGGTGCACTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGTTATCGCGCCCATCTGTAGCGGAGATGATACa                                                      >  2:360324/1‑98 (MQ=38)
                                                                                                                                             cagcagTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCGCCCATCTGTAGCGGAGATGATACAGAACCTTAGGATTGTCCATGTAAACCTCGAAAAGCGCCAAGGAAGGTTCtgt  >  6:289979/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GATCGTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAAAGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTATCGCGCCCATCTGTAGCGGAGATGATACAGAACCTTAGGATTGTCCATGTAAACCTGGAAAAGCGCCAAGGAAGGTTCTGT  >  NC_002947/4224349‑4224638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑