Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,236,548 A→T 7.3% A75A (GCT→GCA) ‡ catA‑I ← catechol 1,2‑dioxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,236,5480AT 7.3% 185.1 / 3.3 64A75A (GCT→GCA) ‡catA‑Icatechol 1,2‑dioxygenase
Reads supporting (aligned to +/- strand):  ref base A (23/36);  new base T (3/2);  total (26/38)
Fisher's exact test for biased strand distribution p-value = 3.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

TCGTCCATGCGCGCTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAGCGCGG  >  NC_002947/4236399‑4236691
                                                                                                                                                     |                                                                                                                                               
tcgtcCATGCGCGCTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCACCCTGCAGCAGGTCGAGGAAGTGCTCGATA‑CCAGgccagca                                                                                                                                                 <  5:119702/149‑1 (MQ=255)
  gtcCATGCGCGCTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGgccagcag                                                                                                                                                >  8:109312/1‑149 (MQ=255)
  gtcCATGCGCGCTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGgccagca                                                                                                                                                 <  7:105019/148‑1 (MQ=255)
      aTGCGCGGTTCGCTCTGGGCCAGCGGCGCCCCTGCTAGGTACAAGGGGCCTTGGATGGTGCGCGGGGTGCCGGCGCCAATGCCGGCTGCGGCATCGTTGGCATCCTGCAGCAGGTCGAGGATGTGCTCGATACCCAGgccagcagccag                                                                                                                                            <  6:379614/149‑1 (MQ=37)
       tGCGCGCTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGgccagcagccag                                                                                                                                            >  6:394855/1‑148 (MQ=255)
          gcgcTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGAGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAgg                                                                                                                                        >  8:282910/1‑149 (MQ=255)
           cgcTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGgc                                                                                                                                       <  5:358735/149‑1 (MQ=255)
                  cgcTGGGCCAGGGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGGTTCGGCATCCTTGGCATCCTGCAGCTGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTc                                                                                                                                <  4:373029/147‑1 (MQ=255)
                  cccTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTc                                                                                                                                >  8:193208/1‑149 (MQ=255)
                  cccTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTc                                                                                                                                >  4:138366/1‑149 (MQ=255)
                   ccTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCg                                                                                                                               >  1:403685/1‑149 (MQ=255)
                       ggCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTAc                                                                                                                           >  4:267085/1‑149 (MQ=255)
                        gCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACg                                                                                                                          <  7:99949/149‑1 (MQ=255)
                        gCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACg                                                                                                                          <  3:267085/149‑1 (MQ=255)
                         ccAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCTGCTGGCTGCTGgcctgg                                                                                                                                   >  4:222813/1‑138 (MQ=25)
                         ccAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCTGCTGGCTGCTGgcctgg                                                                                                                                   <  3:222813/139‑2 (MQ=25)
                         ccAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGgc                                                                                                                                       <  3:119976/135‑1 (MQ=255)
                         ccAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACgg                                                                                                                         >  7:96301/1‑149 (MQ=255)
                         ccAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTAGATACCCAGGCCAGCAGCCAGCAGgc                                                                                                                                       >  4:119976/1‑135 (MQ=255)
                                  ccccGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAgg                                                                                                                <  3:138366/149‑1 (MQ=255)
                                   ccccGCAACGTCCCACTGTCCTGCCATGGGGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCCTCCTGCAGCCGGTCGCGGAAGTGCTCGATCCCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGc                                                                                                               <  4:321415/149‑1 (MQ=255)
                                   cccGGCAACGTACAACGGGCCTTCTATGGTGCGCGGGGTGCCGCCGCCAAGGCAGGCTTCTGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGATCGATAACCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCTCCAAGGc                                                                                                               >  5:361888/1‑149 (MQ=37)
                                       gCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGtt                                                                                                           <  5:394855/149‑1 (MQ=255)
                                        cAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTg                                                                                                          <  5:190729/149‑1 (MQ=255)
                                         aaCGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGa                                                                                                         <  2:116265/149‑1 (MQ=255)
                                         aaCGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGa                                                                                                         <  7:113946/149‑1 (MQ=255)
                                          aCGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAg                                                                                                        <  5:256861/149‑1 (MQ=255)
                                               cAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAg                                                                                                    <  7:282910/148‑1 (MQ=255)
                                                  cGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGa                                                                                                <  7:63025/149‑1 (MQ=255)
                                                    ggCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACg                                                                                              >  5:184580/1‑149 (MQ=255)
                                                        ttCGATGTTGCGCGGGTTGCCGACGCCAAGGCCGGCTTCGGCATCCTTGGAATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCATCATGCCTGCCTCGTTACGGCCGCACAGGCGGTTGACGTAGTCGACGGCGt                                                                                          >  4:374096/1‑149 (MQ=37)
                                                          cGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGt                                                                                          <  2:403685/147‑1 (MQ=255)
                                                          cGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGc                                                                                        >  5:8357/1‑149 (MQ=255)
                                                           gATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGc                                                                                            <  2:292866/144‑1 (MQ=255)
                                                           gATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGc                                                                                            >  1:292866/1‑144 (MQ=255)
                                                           gATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGc                                                                                        <  5:263640/148‑1 (MQ=255)
                                                           gATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGc                                                                                        <  4:128362/148‑1 (MQ=255)
                                                           gATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGc                                                                                        >  3:125480/1‑148 (MQ=255)
                                                             tGGTGCGGGGGGTGCCGCCGCTAAGGCGGGCTTCGGAATCCTTGGCATCCTGGAGCAGTTCGAGGAAGTGCTCGATACCCATGCCTGCAGCCAGCATGCCTGCCTGGTTACTGCCGCCCTGGCGGTTGTTGTAGTCGACGGCGTTCCa                                                                                      <  6:361888/148‑1 (MQ=255)
                                                               gTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGaa                                                                                   <  3:138688/149‑1 (MQ=255)
                                                                tGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAAc                                                                                  <  2:284351/149‑1 (MQ=255)
                                                                  cgcgGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTc                                                                                <  7:146726/149‑1 (MQ=255)
                                                                   gcgGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGTAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGCCGGCGTGCCAGCACTCg                                                                               >  8:264874/1‑149 (MQ=255)
                                                                   gcgGGGTGCCGCCGCAAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCg                                                                               <  3:374096/149‑1 (MQ=255)
                                                                       ggcgccgccgccAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCt                                                                           <  5:370848/146‑1 (MQ=255)
                                                                       ggTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCt                                                                           <  7:193208/149‑1 (MQ=255)
                                                                                   aaGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAAtctc                                                                 >  1:113559/1‑147 (MQ=255)
                                                                                              cGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGAt                                                     >  1:407518/1‑148 (MQ=255)
                                                                                               ggCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATc                                                    <  6:184580/148‑1 (MQ=255)
                                                                                                 cATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAgg                                                 <  8:292838/149‑1 (MQ=255)
                                                                                                     cTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAggcggg                                             <  8:96301/149‑1 (MQ=255)
                                                                                                     cTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAggcggg                                             >  1:305881/1‑149 (MQ=255)
                                                                                                         gCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGGCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGgt                                         <  7:109312/149‑1 (MQ=255)
                                                                                                            tgcTGCAGCTTGTCGAGGATGTGCTGGATACCCAGGCCAGCTGCCAGCAGGCCTGCCTGGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGTACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCTGTGTc                                      <  2:115304/147‑1 (MQ=25)
                                                                                                                gcagcaGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTc                                                        <  2:239593/127‑1 (MQ=255)
                                                                                                                gcagcaGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTc                                                        >  1:239593/1‑127 (MQ=255)
                                                                                                                 cagcagGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTgca                                 <  6:8357/149‑1 (MQ=255)
                                                                                                                        tCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCAcgcg                          >  5:315866/1‑149 (MQ=255)
                                                                                                                           aGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCAcgcg                          >  7:38448/1‑146 (MQ=255)
                                                                                                                                 tGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGAt                 <  1:276457/149‑1 (MQ=255)
                                                                                                                                    tctaTACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGAGTCTTGCAGCACGCGCAGAATGATCTg              >  7:265831/4‑149 (MQ=255)
                                                                                                                                      gATAGCCAGGCCAGCAGGCATCAGGCCTGCCTCGTTACTGCCTCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGc             <  4:172028/148‑1 (MQ=37)
                                                                                                                                      gATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCt            <  1:75510/149‑1 (MQ=255)
                                                                                                                                       cgaggcAGGCCTGCTGGCTGCTGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCtt           >  5:79380/6‑149 (MQ=25)
                                                                                                                                        tACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCATGTCTTCGATCAGGCGGTCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTg          <  8:370994/149‑1 (MQ=255)
                                                                                                                                          cccAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGaa        <  2:305881/149‑1 (MQ=255)
                                                                                                                                           ccAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAg       >  7:296022/1‑149 (MQ=255)
                                                                                                                                           ccAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAg       >  8:55734/1‑149 (MQ=255)
                                                                                                                                             aGGCCTGCTGGCTGCTGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAgcg     >  5:80612/1‑149 (MQ=25)
                                                                                                                                               gccagcagccagcaGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCtt                                                         <  3:118792/95‑1 (MQ=255)
                                                                                                                                               gccagcagccagcaGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCtt                                                         >  4:118792/1‑95 (MQ=255)
                                                                                                                                               gccagcagccagcaGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAGcgcg   >  4:157776/1‑149 (MQ=255)
                                                                                                                                                ccagcagccagcagGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAGCGCgg  <  8:38448/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
TCGTCCATGCGCGCTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAGCGCGG  >  NC_002947/4236399‑4236691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑