Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,236,550	C→G	7.4%	A75P (GCT→CCT) ‡	catA‑I ←	catechol 1,2‑dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,236,550	0	C	G	7.4%	201.9 / 4.5	64	A75P (GCT→CCT) ‡	catA‑I	catechol 1,2‑dioxygenase
Reads supporting (aligned to +/- strand):  ref base C (23/36);  new base G (3/2);  total (26/38)
Fisher's exact test for biased strand distribution p-value = 3.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCGCGCTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAGCGCGG  >  NC_002947/4236405‑4236691                                                                                                                                                  |                                                                                                                                              aTGCGCGGTTCGCTCTGGGCCAGCGGCGCCCCTGCTAGGTACAAGGGGCCTTGGATGGTGCGCGGGGTGCCGGCGCCAATGCCGGCTGCGGCATCGTTGGCATCCTGCAGCAGGTCGAGGATGTGCTCGATACCCAGgccagcagccag                                                                                                                                            <  6:379614/149‑1 (MQ=37)  tGCGCGCTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGgccagcagccag                                                                                                                                            >  6:394855/1‑148 (MQ=255)     gcgcTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGAGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAgg                                                                                                                                        >  8:282910/1‑149 (MQ=255)      cgcTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGgc                                                                                                                                       <  5:358735/149‑1 (MQ=255)             cgcTGGGCCAGGGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGGTTCGGCATCCTTGGCATCCTGCAGCTGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTc                                                                                                                                <  4:373029/147‑1 (MQ=255)             cccTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTc                                                                                                                                >  8:193208/1‑149 (MQ=255)             cccTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTc                                                                                                                                >  4:138366/1‑149 (MQ=255)              ccTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCg                                                                                                                               >  1:403685/1‑149 (MQ=255)                  ggCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTAc                                                                                                                           >  4:267085/1‑149 (MQ=255)                   gCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACg                                                                                                                          <  7:99949/149‑1 (MQ=255)                   gCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACg                                                                                                                          <  3:267085/149‑1 (MQ=255)                    ccAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCTGCTGGCTGCTGgcctgg                                                                                                                                   >  4:222813/1‑138 (MQ=25)                    ccAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCTGCTGGCTGCTGgcctgg                                                                                                                                   <  3:222813/139‑2 (MQ=25)                    ccAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGgc                                                                                                                                       <  3:119976/135‑1 (MQ=255)                    ccAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACgg                                                                                                                         >  7:96301/1‑149 (MQ=255)                    ccAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTAGATACCCAGGCCAGCAGCCAGCAGgc                                                                                                                                       >  4:119976/1‑135 (MQ=255)                             ccccGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAgg                                                                                                                <  3:138366/149‑1 (MQ=255)                              ccccGCAACGTCCCACTGTCCTGCCATGGGGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCCTCCTGCAGCCGGTCGCGGAAGTGCTCGATCCCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGc                                                                                                               <  4:321415/149‑1 (MQ=255)                              cccGGCAACGTACAACGGGCCTTCTATGGTGCGCGGGGTGCCGCCGCCAAGGCAGGCTTCTGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGATCGATAACCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCTCCAAGGc                                                                                                               >  5:361888/1‑149 (MQ=37)                                  gCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGtt                                                                                                           <  5:394855/149‑1 (MQ=255)                                   cAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTg                                                                                                          <  5:190729/149‑1 (MQ=255)                                    aaCGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGa                                                                                                         <  7:113946/149‑1 (MQ=255)                                    aaCGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGa                                                                                                         <  2:116265/149‑1 (MQ=255)                                     aCGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAg                                                                                                        <  5:256861/149‑1 (MQ=255)                                          cAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAg                                                                                                    <  7:282910/148‑1 (MQ=255)                                             cGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGa                                                                                                <  7:63025/149‑1 (MQ=255)                                               ggCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACg                                                                                              >  5:184580/1‑149 (MQ=255)                                                   ttCGATGTTGCGCGGGTTGCCGACGCCAAGGCCGGCTTCGGCATCCTTGGAATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCATCATGCCTGCCTCGTTACGGCCGCACAGGCGGTTGACGTAGTCGACGGCGt                                                                                          >  4:374096/1‑149 (MQ=37)                                                     cGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGt                                                                                          <  2:403685/147‑1 (MQ=255)                                                     cGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGc                                                                                        >  5:8357/1‑149 (MQ=255)                                                      gATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGc                                                                                            <  2:292866/144‑1 (MQ=255)                                                      gATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGc                                                                                            >  1:292866/1‑144 (MQ=255)                                                      gATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGc                                                                                        >  3:125480/1‑148 (MQ=255)                                                      gATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGc                                                                                        <  4:128362/148‑1 (MQ=255)                                                      gATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGc                                                                                        <  5:263640/148‑1 (MQ=255)                                                        tGGTGCGGGGGGTGCCGCCGCTAAGGCGGGCTTCGGAATCCTTGGCATCCTGGAGCAGTTCGAGGAAGTGCTCGATACCCATGCCTGCAGCCAGCATGCCTGCCTGGTTACTGCCGCCCTGGCGGTTGTTGTAGTCGACGGCGTTCCa                                                                                      <  6:361888/148‑1 (MQ=255)                                                          gTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGaa                                                                                   <  3:138688/149‑1 (MQ=255)                                                           tGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAAc                                                                                  <  2:284351/149‑1 (MQ=255)                                                             cgcgGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTc                                                                                <  7:146726/149‑1 (MQ=255)                                                              gcgGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGTAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGCCGGCGTGCCAGCACTCg                                                                               >  8:264874/1‑149 (MQ=255)                                                              gcgGGGTGCCGCCGCAAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCg                                                                               <  3:374096/149‑1 (MQ=255)                                                                  ggcgccgccgccAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCt                                                                           <  5:370848/146‑1 (MQ=255)                                                                  ggTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCt                                                                           <  7:193208/149‑1 (MQ=255)                                                                              aaGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAAtctc                                                                 >  1:113559/1‑147 (MQ=255)                                                                                         cGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGAt                                                     >  1:407518/1‑148 (MQ=255)                                                                                          ggCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATc                                                    <  6:184580/148‑1 (MQ=255)                                                                                            cATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAgg                                                 <  8:292838/149‑1 (MQ=255)                                                                                                cTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAggcggg                                             <  8:96301/149‑1 (MQ=255)                                                                                                cTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAggcggg                                             >  1:305881/1‑149 (MQ=255)                                                                                                    gCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGGCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGgt                                         <  7:109312/149‑1 (MQ=255)                                                                                                       tgcTGCAGCTTGTCGAGGATGTGCTGGATACCCAGGCCAGCTGCCAGCAGGCCTGCCTGGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGTACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCTGTGTc                                      <  2:115304/147‑1 (MQ=25)                                                                                                           gcagcaGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTc                                                        >  1:239593/1‑127 (MQ=255)                                                                                                           gcagcaGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTc                                                        <  2:239593/127‑1 (MQ=255)                                                                                                            cagcagGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTgca                                 <  6:8357/149‑1 (MQ=255)                                                                                                                   tCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCAcgcg                          >  5:315866/1‑149 (MQ=255)                                                                                                                      aGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCAcgcg                          >  7:38448/1‑146 (MQ=255)                                                                                                                            tGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGAt                 <  1:276457/149‑1 (MQ=255)                                                                                                                               tctaTACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGAGTCTTGCAGCACGCGCAGAATGATCTg              >  7:265831/4‑149 (MQ=255)                                                                                                                                 gATAGCCAGGCCAGCAGGCATCAGGCCTGCCTCGTTACTGCCTCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGc             <  4:172028/148‑1 (MQ=37)                                                                                                                                 gATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCt            <  1:75510/149‑1 (MQ=255)                                                                                                                                  cgaggcAGGCCTGCTGGCTGCTGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCtt           >  5:79380/6‑149 (MQ=25)                                                                                                                                   tACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCATGTCTTCGATCAGGCGGTCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTg          <  8:370994/149‑1 (MQ=255)                                                                                                                                     cccAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGaa        <  2:305881/149‑1 (MQ=255)                                                                                                                                      ccAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAg       >  7:296022/1‑149 (MQ=255)                                                                                                                                      ccAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAg       >  8:55734/1‑149 (MQ=255)                                                                                                                                        aGGCCTGCTGGCTGCTGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAgcg     >  5:80612/1‑149 (MQ=25)                                                                                                                                          gccagcagccagcaGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCtt                                                         <  3:118792/95‑1 (MQ=255)                                                                                                                                          gccagcagccagcaGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCtt                                                         >  4:118792/1‑95 (MQ=255)                                                                                                                                          gccagcagccagcaGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAGcgcg   >  4:157776/1‑149 (MQ=255)                                                                                                                                           ccagcagccagcagGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAGCGCgg  <  8:38448/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                              ATGCGCGCTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAGCGCGG  >  NC_002947/4236405‑4236691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑