Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,252,213 T→G 6.4% intergenic (‑181/+59) PP_3726 ← / ← rocE enoyl‑CoA hydratase/isomerase family protein/amino acid permease RocE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,252,2130TG 6.4% 226.9 / 4.0 73intergenic (‑181/+59)PP_3726/rocEenoyl‑CoA hydratase/isomerase family protein/amino acid permease RocE
Reads supporting (aligned to +/- strand):  ref base T (37/31);  new base G (3/2);  total (40/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTGATGAGGGAGATACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAGCACAGGGCAATGAAG  >  NC_002947/4252069‑4252356
                                                                                                                                                |                                                                                                                                               
cTTGATGAGGGAGATACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGAGGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGAc                                                                                                                                             >  8:85707/1‑149 (MQ=255)
   gatgaGGGAGATACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCt                                                                                                                                           <  3:162806/148‑1 (MQ=255)
   gatgaGGGAGATACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCt                                                                                                                                           >  8:102854/1‑148 (MQ=255)
     tgaGGGAGATACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGTGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTca                                                                                                                                         <  3:51652/148‑1 (MQ=255)
        gggAGATACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGGTCGAGCGCCGCCTACGCGGTGCGCGATCTCACAgg                                                                                                                                     <  1:292181/149‑1 (MQ=38)
         ggAGATACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCCCAGgc                                                                                                                                    >  4:209004/1‑149 (MQ=255)
              tACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGa                                                                                                                               <  1:100833/149‑1 (MQ=255)
               aCTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGa                                                                                                                               >  7:382042/1‑148 (MQ=255)
                   aaCGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGAc                                                                                                                                             <  4:363119/130‑1 (MQ=255)
                   aaCGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGAc                                                                                                                                             >  3:363119/1‑130 (MQ=255)
                   aaCGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATg                                                                                                                          >  8:296949/1‑149 (MQ=255)
                      gAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAg                                                                                                                       >  6:399423/1‑149 (MQ=255)
                           ttCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGAc                                                                                                                   >  2:212919/1‑148 (MQ=255)
                                    gCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTAACACGCGCTGAAAATGCAGCGACAAACACCTGc                                                                                                         >  8:132825/1‑149 (MQ=255)
                                             gCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGAc                                                                                                                   <  1:66622/130‑1 (MQ=255)
                                             gCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGAc                                                                                                                   >  2:66622/1‑130 (MQ=255)
                                             gCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCcgacgacga                                                                                                >  3:39719/1‑149 (MQ=255)
                                             gCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCcgacgac                                                                                                  >  5:111671/1‑147 (MQ=255)
                                                 ttACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTg                                                                                            >  3:402974/1‑149 (MQ=255)
                                                    cAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTgcg                                                                                          <  7:296949/148‑1 (MQ=255)
                                                     aaCTTCGCAGAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTgcggc                                                                                        <  4:39719/149‑1 (MQ=255)
                                                     aaCTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTgcggc                                                                                        <  4:402974/149‑1 (MQ=255)
                                                        ttCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGt                                                                                      >  8:249853/1‑148 (MQ=255)
                                                           gCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGATAATGCAGCGAGCAACACCTGCCGCCGACGAGCTGCGGCGTCaaa                                                                                  >  6:198556/1‑149 (MQ=255)
                                                               aaCTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCcga                                                                                                      >  3:389283/1‑125 (MQ=255)
                                                               aaCTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCcga                                                                                                      <  4:389283/125‑1 (MQ=255)
                                                                 cTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTg                                                                            >  2:250607/1‑149 (MQ=255)
                                                                  tCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAAc                                                                                 >  3:350131/1‑143 (MQ=255)
                                                                  tCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAAc                                                                                 <  4:350131/143‑1 (MQ=255)
                                                                    ggTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGgt                                                                          <  2:407125/148‑1 (MQ=255)
                                                                    ggTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGgt                                                                          <  1:297619/148‑1 (MQ=255)
                                                                         cTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTg                                                                    <  7:85707/149‑1 (MQ=255)
                                                                          tGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTgg                                                                   >  6:94742/1‑149 (MQ=255)
                                                                          tGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTgg                                                                   >  8:131385/1‑149 (MQ=255)
                                                                           ggCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTggg                                                                  >  1:214214/1‑149 (MQ=255)
                                                                            gCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCGGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGt                                                                 <  1:25296/149‑1 (MQ=255)
                                                                              gTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGc                                                               >  5:77891/1‑149 (MQ=255)
                                                                                  cacaAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAg                                                           >  3:55206/1‑149 (MQ=255)
                                                                                   acaAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAgg                                                          >  8:349616/1‑149 (MQ=255)
                                                                                    caAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGc                                                         <  4:55206/149‑1 (MQ=255)
                                                                                     aaGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGcc                                                        <  5:399423/149‑1 (MQ=255)
                                                                                       gggCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGcccc                                                      >  6:66633/1‑149 (MQ=255)
                                                                                        ggCGTTCGTCATGACGGCTTTAGAGCATGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCAAAGGCGATGAAAATGCAGAGACAAACACATCCCGACGACGAGCTGCGCCGTAAAACCCATGGTGTGATGGGTGCGAAGGcccc                                                      >  7:256560/1‑148 (MQ=255)
                                                                                           gTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTgcggc                                                                                        <  1:144810/111‑1 (MQ=255)
                                                                                           gTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTgcggc                                                                                        >  2:144810/1‑111 (MQ=255)
                                                                                                tCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGAcg                                             >  4:370014/1‑149 (MQ=255)
                                                                                                 cATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGccc                                                       <  6:193389/138‑1 (MQ=255)
                                                                                                 cATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGccc                                                       >  5:193389/1‑138 (MQ=255)
                                                                                                  aTGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCaaa                                                                                  >  5:303647/1‑110 (MQ=255)
                                                                                                  aTGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCaaa                                                                                  <  6:303647/110‑1 (MQ=255)
                                                                                                  aTGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGAcgg                                            <  2:214214/148‑1 (MQ=255)
                                                                                                     aCGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGAcggcgg                                         <  5:66633/148‑1 (MQ=255)
                                                                                                      cGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGtt                                       <  7:249853/149‑1 (MQ=255)
                                                                                                      cGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGtt                                       <  6:111671/149‑1 (MQ=255)
                                                                                                       ggCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGtt                                       >  2:386312/1‑148 (MQ=255)
                                                                                                              tGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACgaag                                <  8:256560/148‑1 (MQ=255)
                                                                                                                 ccTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGt                            <  5:94742/149‑1 (MQ=255)
                                                                                                                  cTGTGAGGTCGCGCGCCGCCTACGCGGTGCGCGATCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTa                           >  5:76739/1‑149 (MQ=38)
                                                                                                                  cTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAATACCGGGCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTa                           >  1:324674/1‑149 (MQ=255)
                                                                                                                   tgtgAGGTCGAGCGCCGCCTACGCGGTGCGCGATCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACgaagaa                              >  7:171158/1‑145 (MQ=38)
                                                                                                                   tgtgAGGTCGAGCGCCGCCTACGCGGTGCGCGATCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACgaagaa                              <  8:171158/145‑1 (MQ=38)
                                                                                                                   tgtgAGGTCGAGCGCCGCCTACGCGGTGCGCGATCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTAc                          >  1:238547/1‑149 (MQ=38)
                                                                                                                   tgtgAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGAcgg                                            <  3:2535/131‑1 (MQ=255)
                                                                                                                   tgtgAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGAcgg                                            >  4:2535/1‑131 (MQ=255)
                                                                                                                   tgtgAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTAc                          >  3:377389/1‑149 (MQ=255)
                                                                                                                     tgAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTAcgc                        <  7:102854/149‑1 (MQ=255)
                                                                                                                       agaTCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGa                                     <  7:82842/134‑1 (MQ=255)
                                                                                                                       agaTCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGa                                     >  8:82842/1‑134 (MQ=255)
                                                                                                                        gaTCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTAcgccg                      >  4:151917/1‑148 (MQ=255)
                                                                                                                            gcgcACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATa                  <  7:132825/148‑1 (MQ=255)
                                                                                                                                   gcgTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAGCACAggg          <  4:377389/149‑1 (MQ=255)
                                                                                                                                         gcggcgCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAGCACAGGGCAATg     <  7:131385/148‑1 (MQ=255)
                                                                                                                                           ggcgCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAGCACAGGGCAATGAAg  >  8:291179/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CTTGATGAGGGAGATACTAAACGAGTGTTCAGCAAGGCGGGATGGGCTGTTACAACTTCGCACAACTCGGTGTCTGGCGTTACACAAGGGCGTTCGTCATGACGGCTTTATGGCCTGTGAGATCGCGCACCGCGTAGGCGGCGCTCGACCTCACAGGCGCTGAAAATGCAGCGACCAACACCTGCCGACGACGAGCTGCGGCGTCAAACCCCTGGTGTGCTGGGTGCGAAGGCCCCCTGCCGACGGCGGTTGACGAAGAAGTACGCCGCATAGCACAGGGCAATGAAG  >  NC_002947/4252069‑4252356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑