| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,271,005 | T→C | 7.3% | I382T (ATC→ACC) | mrdA‑I → | transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,271,005 | 0 | T | C | 7.3% | 156.4 / 2.6 | 55 | I382T (ATC→ACC) | mrdA‑I | transpeptidase |
| Reads supporting (aligned to +/- strand): ref base T (24/27); new base C (2/2); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01 | |||||||||||
AAGTGGCCATCGCTGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTGGGCCAGAAGGTGTCGCTGGACATGTTCGAGGAAGCCTCCGGGCTGATGCCCTCGGCC > NC_002947/4270858‑4271150 | tcgaggCCAGCGATGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCcat > 4:274786/5‑148 (MQ=255)tcgaggCCAGCGATGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCcat > 2:367729/5‑148 (MQ=255) aGTGGCCATCGCTGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCcatca < 1:191047/149‑1 (MQ=255) ccATCGCTGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGt < 2:279232/149‑1 (MQ=255) cATCGCTGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGtt < 7:72212/149‑1 (MQ=255) cTGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAAc < 8:7334/149‑1 (MQ=255) tCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACAcc > 1:215255/1‑149 (MQ=255) aCAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTAc < 5:171475/149‑1 (MQ=255) aCAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTAc > 8:204943/1‑149 (MQ=255) aGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTCCtt > 5:3631/1‑149 (MQ=255) gggTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTAc < 6:302685/149‑1 (MQ=255) cACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTgg > 1:395113/1‑149 (MQ=255) aCCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGgc > 2:165003/1‑149 (MQ=255) aCCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGgc > 2:365259/1‑149 (MQ=255) cccTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTa < 1:368370/132‑1 (MQ=255) cccTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTa > 2:368370/1‑132 (MQ=255) ggTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCa < 1:365259/149‑1 (MQ=255) aCCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCgg > 2:213718/1‑149 (MQ=255) cccGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGc < 2:215255/149‑1 (MQ=255) gCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCAc < 7:2244/149‑1 (MQ=255) ctacGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAAc > 7:37849/1‑94 (MQ=255) ctacGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAAc < 8:37849/94‑1 (MQ=255) gAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACAt > 6:297817/1‑149 (MQ=255) aaCTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACTGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCGCAAGTTGGGCATCGACCGGCTGCACGACTACATg > 2:54335/1‑149 (MQ=255) aaCTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATg < 3:274786/149‑1 (MQ=255) cccAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑cc > 2:213810/1‑147 (MQ=255) ccAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAg > 3:26117/1‑149 (MQ=255) cAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATc > 3:188576/1‑121 (MQ=255) cAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATc < 4:188576/121‑1 (MQ=255) ccACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTgg < 1:367729/149‑1 (MQ=255) cacaAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTggg > 6:101867/1‑149 (MQ=255) cacaAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTggg > 5:115523/1‑149 (MQ=255) cacaAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTgg > 5:20876/1‑148 (MQ=255) cacaAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTgg < 6:20876/148‑1 (MQ=255) acaAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTGGGc < 4:26117/149‑1 (MQ=255) cGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTGGGCCAGAAGgt < 1:213718/149‑1 (MQ=255) cAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTGGGCCAGAAGgtgt < 6:115523/149‑1 (MQ=255) cAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTGGGCCAGAAGgtgt < 7:204943/149‑1 (MQ=255) gAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTGGGCCAGAAGGTGTCGCTg < 6:152780/148‑1 (MQ=255) aCCGCAGCGGCGACGGCTGGGTGGACATGTACACTGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCGCAAGTTGGGCATCGACCGGCTGCACGACTACATGGACCGAGTTCGGCCTGGGCCAGAAGGTGTCGCTGGa < 1:54335/149‑1 (MQ=255) ccGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTGGGCCAGAAg < 2:168237/136‑1 (MQ=255) ccGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTGGGCCAGAAg > 1:168237/1‑136 (MQ=255) cGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACCAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTGGGCCAGAAGGTGTCGCTGGACAt < 6:3631/149‑1 (MQ=255) cggcgACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTGGGCCAGAAGGTGTCGCTGGACATGTTCg < 8:7311/149‑1 (MQ=255) aCGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTGGGCCAGAAGGTGTCGCTGGACATGTTCGAGGaa < 2:395113/149‑1 (MQ=255) gCTGGGTGGACATGTACACCGCCACCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACa < 5:176070/96‑1 (MQ=255) gCTGGGTGGACATGTACACCGCCACCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACa < 5:169896/96‑1 (MQ=255) gCTGGGTGGACATGTACACCGCCACCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACa > 6:169896/1‑96 (MQ=255) gCTGGGTGGACATGTACACCGCCACCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACa > 6:176070/1‑96 (MQ=255) gTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTGGGCCAGAAGGTGTCGCTGGACATGTTCGAGGAAGCCTCCgg < 5:101867/149‑1 (MQ=255) aTGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTGGGCCAGAAGGTGTCGCTGGACATGTTCGAGGAAGCCTCCGGGCTGAt > 4:367817/1‑149 (MQ=255) tGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCACACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTGGGCCAGAAGGTGTCGCTGGACATGTTCGAGGAAGCCTCCGGGCTGATg > 3:404345/1‑149 (MQ=255) gTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTGGGCCAGAAGGTGTCGCTgg < 6:370088/119‑1 (MQ=255) gTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTGGGCCAGAAGGTGTCGCTgg > 5:370088/1‑119 (MQ=255) tACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTGGGCCAGAAGGTGTCGCTGGACATGTTCGAGGAAGCCTCCGGGCTGATGcc > 3:129629/1‑149 (MQ=255) tACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTGGGCCAGAAGGTGTCGCTGGACATGTTCGAGGAAGCCTCCGGGCTGATGcc > 8:99156/1‑149 (MQ=255) accaccATCATGCGTTCCAACGCCACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTGGGCCAGAAGGTGACGCTGGACATGTTCGAGGAAGCCTCCGGGCTGCTTCCCTc > 7:334256/1‑149 (MQ=255) ccATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTGGGCCAGAAGGTGTCGCTGGACATGTTCGAGGAAGCCTCCGGGCTGATGCCCTCGGcc < 7:99156/149‑1 (MQ=255) catcatGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTGGGCCAGAAGGTGTCGCTGGACATGTTCGa > 3:394437/1‑120 (MQ=255) catcatGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTAAATGG‑CCGAGTTCGGCCTGGGCCAGAAGGTGTCGCTCGACATGTTCGAGGAAGCCTCCGGGCTGCTGCCCTCGGcc > 8:136664/1‑148 (MQ=255) | AAGTGGCCATCGCTGGCCTCGACAGCGGGGTGATCACCCCTGGCAGCCGGGTGTTCGACCCGGGCTACTACGAACTGCCCAACTATGACCACAAGTACCGCAACTGGAACCGCAGCGGCGACGGCTGGGTGGACATGTACACCGCCATCATGCGTTCCAACGACACCTACTTCTACGACCTGGCGCACAAGTTGGGCATCGACCGGCTGCACGACTACATGG‑CCGAGTTCGGCCTGGGCCAGAAGGTGTCGCTGGACATGTTCGAGGAAGCCTCCGGGCTGATGCCCTCGGCC > NC_002947/4270858‑4271150 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |