Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,282,382 A→G 5.7% V283A (GTG→GCG)  PP_3753 ← AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,282,3820AG 5.7% 206.4 / 2.5 72V283A (GTG→GCG) PP_3753AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (31/35);  new base G (2/2);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

AGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTCCATAGCGCAATGCCCGCAGGTGCCGATACACCTCA  >  NC_002947/4282245‑4282528
                                                                                                                                         |                                                                                                                                                  
aGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTAGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCtt                                                                                                                                          >  1:104381/1‑148 (MQ=255)
 gCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGa                                                                                                                                        <  2:126583/149‑1 (MQ=255)
  cTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGAt                                                                                                                                       <  3:212551/149‑1 (MQ=255)
    cccTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCGCTTCATCCTTGATct                                                                                                                                     >  4:100065/1‑149 (MQ=255)
    cccTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCGCTTCATCCTTGATct                                                                                                                                     >  4:94466/1‑149 (MQ=255)
    cccTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATct                                                                                                                                     <  3:31741/149‑1 (MQ=255)
      cTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATctgc                                                                                                                                   <  4:321887/149‑1 (MQ=255)
         ggCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATctgctg                                                                                                                                 <  7:360105/148‑1 (MQ=255)
         ggCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATctgctg                                                                                                                                 <  5:95286/148‑1 (MQ=255)
                  cGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGGTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGc                                                                                                                       <  3:212544/149‑1 (MQ=255)
                    gTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTAATCCTTAATATGATGGTAAGAACGcaa                                                                                                                     >  8:58193/1‑147 (MQ=255)
                      ccACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATctg                                                                                                                                    <  2:23350/132‑1 (MQ=255)
                      ccACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATctg                                                                                                                                    >  1:23350/1‑132 (MQ=255)
                        aCTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTAc                                                                                                                             <  4:115672/137‑1 (MQ=255)
                        aCTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTAc                                                                                                                             >  3:115672/1‑137 (MQ=255)
                        aCTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTc                                                                                                                  >  6:212437/1‑148 (MQ=255)
                        aCTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTc                                                                                                                  <  5:212437/148‑1 (MQ=255)
                         cttcttGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGcc                                                                                                                      <  8:104041/143‑1 (MQ=255)
                         cttcttGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGcc                                                                                                                      >  7:104041/1‑143 (MQ=255)
                            cttGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCCGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGCCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACCAACGCCCCTCCCGCt                                                                                                             >  4:121305/1‑149 (MQ=255)
                             ttGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTc                                                                                                            >  8:147391/1‑149 (MQ=255)
                             ttGAACGCCCGGTGGAAGGCACTCGGTACCTGAAACCCCGCCAGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGCCGCACTTCATCCATGATCTGCTGGTACGAACGCCCCTCCCGCTc                                                                                                            >  3:38139/1‑149 (MQ=255)
                               gAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTcc                                                                                                           >  6:226513/1‑148 (MQ=255)
                               gAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTcc                                                                                                           >  8:29771/1‑148 (MQ=255)
                                aaCGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCaa                                                                                                         >  1:150029/1‑149 (MQ=255)
                                aaCGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCaa                                                                                                         >  5:104438/1‑149 (MQ=255)
                                      cGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCg                                                                                                   <  7:29771/149‑1 (MQ=255)
                                       ggTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCgg                                                                                                  <  2:150029/149‑1 (MQ=255)
                                        gTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGc                                                                                                 <  7:229407/149‑1 (MQ=255)
                                                 aCTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTAc                                                                                        <  7:147391/149‑1 (MQ=255)
                                                  cTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACt                                                                                       >  7:304946/1‑149 (MQ=255)
                                                   tCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTg                                                                                      >  3:63160/1‑149 (MQ=255)
                                                       ttCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCAc                                                                                  <  8:304946/149‑1 (MQ=255)
                                                       ttCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCAc                                                                                  <  7:25311/149‑1 (MQ=255)
                                                          cTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGa                                                                               <  8:174544/149‑1 (MQ=255)
                                                          cTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGa                                                                               >  1:400182/1‑149 (MQ=255)
                                                           tGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGAt                                                                              >  2:27050/1‑149 (MQ=255)
                                                            gAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATc                                                                             >  4:248617/1‑149 (MQ=255)
                                                             aaaCCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAg                                                                                            >  6:410388/1‑133 (MQ=255)
                                                             aaaCCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAg                                                                                            <  5:410388/133‑1 (MQ=255)
                                                              aaCCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATccc                                                                           <  1:346455/149‑1 (MQ=255)
                                                              aaCCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATccc                                                                           <  2:104381/149‑1 (MQ=255)
                                                                 cccGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTg                                                                        <  5:407229/149‑1 (MQ=255)
                                                                 cccGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTg                                                                        <  3:121305/149‑1 (MQ=255)
                                                                         cTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTgcg                                                                >  1:400208/1‑149 (MQ=255)
                                                                          tCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCt                                                                                                                   >  5:77557/1‑97 (MQ=255)
                                                                          tCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCt                                                                                                                   <  6:77557/97‑1 (MQ=255)
                                                                                 tttCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCg                                                        <  3:231648/149‑1 (MQ=255)
                                                                                 tttCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTACTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCg                                                        >  4:144358/1‑149 (MQ=255)
                                                                                    cGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTAc                                                                                        >  2:219862/1‑114 (MQ=255)
                                                                                    cGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGc                                                      <  1:301551/148‑1 (MQ=255)
                                                                                     ggCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCGCTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGcc                                                     <  3:94466/148‑1 (MQ=255)
                                                                                     ggCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCGCTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGcc                                                     <  3:100065/148‑1 (MQ=255)
                                                                                        aaTGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCCCTTCATCCTTGATCTGCTGGTAGGAACGCCCCTCCCGCTCCAACTGACGGCGTAAGGTAATGGCACTGATCCCGACTTGCTGCGCCAGTGCGTCCAATg                                                 >  6:36473/1‑149 (MQ=255)
                                                                                            cTCAACGCGCCTTCTCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCg                                              <  7:58193/148‑1 (MQ=255)
                                                                                              cAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCTCTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGc                                            >  4:88297/1‑148 (MQ=255)
                                                                                                aCGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCAc                                         >  4:290876/1‑149 (MQ=255)
                                                                                                  gcgcCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCCTCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCACCTGCCGGCGAAAGGGACTGGCCCGGCTCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGccccgg                                       >  8:195098/1‑145 (MQ=255)
                                                                                                  gcgcCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTg                                       <  6:104438/149‑1 (MQ=255)
                                                                                                  gcgcCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTg                                       >  8:207490/1‑149 (MQ=255)
                                                                                                  gcgcCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTg                                       >  5:217111/1‑149 (MQ=255)
                                                                                                         cgcgCAGCCGCTCGAAGGCCATGGCCCGGCTCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTCCATa                                 <  3:248617/148‑1 (MQ=255)
                                                                                                                 cGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTCCATAGCGCAATGc                        >  5:339615/1‑149 (MQ=255)
                                                                                                                  gCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTCTTGCTGCGCCAGTGCGGCCAATGACGGCCACTGTCCAAAGCGCAATGcc                       >  1:387076/1‑149 (MQ=255)
                                                                                                                       cagggcctGGCCCGGCGCACTTCATGCTTGATCTGCTTGTACGAACGCCCCTGCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCGTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTCCATAGCGCAATGCCCGCAg                  <  4:38139/142‑1 (MQ=255)
                                                                                                                       aaGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTCCATAGCGCAATGCCCGCAg                  >  2:126975/1‑149 (MQ=255)
                                                                                                                       aaGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTCCATAGCGCAATGCCCGCAg                  >  4:240398/1‑149 (MQ=255)
                                                                                                                       aaGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGGACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTCCATAGCGCAATGCCCGCAg                  <  6:217111/149‑1 (MQ=255)
                                                                                                                           ccATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTCCATAGCGCAATGCCCGCAGGTGc              <  6:339615/149‑1 (MQ=255)
                                                                                                                              tGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTCCATAGCGCAATGCCCGCAGGTGCCGa           <  7:207490/149‑1 (MQ=255)
                                                                                                                                  ccGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTCCATAGCGCAATGCCCGCAGGTGCCGATaca       <  2:400208/149‑1 (MQ=255)
                                                                                                                                       gcACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTCCATAGCGCAATGCCCGCAGGTGCCGATACACCTCa  <  4:63160/149‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                  
AGCTCCCTGGGCTCTGCCCGGTCCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCATCCTTGATCTGCTGGTACGAACGCCCCTCCCGCTCCAACTGCCGGCGAAAGGTACTGGCACTGATCCCCTGTTGCTGCGCCAGTGCGGCCAATGTCGGCCACTGTCCATAGCGCAATGCCCGCAGGTGCCGATACACCTCA  >  NC_002947/4282245‑4282528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑