Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,289,430 T→G 7.5% K1053Q (AAG→CAG)  PP_3761 ← two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,289,4300TG 7.5% 188.7 / 4.2 64K1053Q (AAG→CAG) PP_3761two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base T (30/29);  new base G (2/3);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTCGACCTTGTG  >  NC_002947/4289282‑4289577
                                                                                                                                                    |                                                                                                                                                   
caaccgcgCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCtt                                                                                                                                                     <  2:187990/142‑1 (MQ=255)
        cTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTc                                                                                                                                               >  6:365137/1‑147 (MQ=255)
         tGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAg                                                                                                                                            >  3:26234/1‑149 (MQ=255)
           gCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAggg                                                                                                                                          <  5:294899/149‑1 (MQ=255)
             ggATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGgcg                                                                                                                                        <  4:305598/149‑1 (MQ=255)
             ggATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTGGAGCACAAGGgcg                                                                                                                                        >  8:117948/1‑149 (MQ=255)
               aTCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCT‑CAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTg                                                                                                                                     <  8:177393/149‑1 (MQ=255)
                tCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTGGAGCACAAGGgcgcca                                                                                                                                     <  7:117948/149‑3 (MQ=255)
                gcAGGCGGGTGGCCTGGATCCCGTGCATCTGCGGCTTCATGACGTCCATCAGGAGCAGGTCGATTTCTTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTGGAGCACAAGGgcgcca                                                                                                                                     <  8:180474/148‑3 (MQ=255)
                   ggCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCCGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGt                                                                                                                                   >  6:356802/1‑148 (MQ=255)
                      gggTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAgg                                                                                                                               <  5:228488/149‑1 (MQ=255)
                           gacTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTACAGGCGCTCGATGGCTTCGCGACCGTTGCTGCAGATTTCGACAATTGCGCCCTTGTGCTCCAGGGCGCTGGTCAGTGCaa                                                                                                                           >  6:122732/3‑148 (MQ=255)
                            ccTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCa                                                                                                                            >  5:226668/1‑146 (MQ=255)
                                 aaGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGGCAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATAtt                                                                                                                    <  1:342154/149‑1 (MQ=255)
                                     cGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCTATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTTCGGCCGATTTCGACAATGTCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGc                                                                                                                <  5:122732/149‑1 (MQ=255)
                                     cGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGc                                                                                                                <  2:197628/149‑1 (MQ=255)
                                     cGTCCATCTCCGGCATCATCACGTCCATCAGAACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACg                                                                                                                 >  4:141335/1‑148 (MQ=255)
                                      gTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCa                                                                                                                                             <  7:217659/119‑1 (MQ=255)
                                      gTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCa                                                                                                                                             >  8:217659/1‑119 (MQ=255)
                                      gTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCaaa                                                                                                                          <  6:113637/138‑1 (MQ=255)
                                      gTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCaa                                                                                                                           <  4:240955/137‑1 (MQ=255)
                                      gTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCaa                                                                                                                           >  3:240955/1‑137 (MQ=255)
                                      gTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCCTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCaaa                                                                                                                          >  5:113637/1‑138 (MQ=255)
                                           tctcCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACAtcg                                                                                                          >  5:217837/1‑149 (MQ=255)
                                              ccGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACAtcgtc                                                                                                        <  4:26234/148‑1 (MQ=255)
                                               cGGCATCATCACGTCCATCAGCACCAGGTCGATATAGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCACTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGCTATTAAGCACAtcgtcg                                                                                                       >  6:53266/1‑148 (MQ=255)
                                                     catcaCGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTccacca                                                                                                >  5:151049/1‑149 (MQ=255)
                                                       tcaCGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACcag                                                                                               >  6:145753/1‑148 (MQ=255)
                                                          cGTCCATCAGCACCAGGTCGATATCGTCGTGCTTCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCTGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCagcagc                                                                                           <  5:53266/149‑1 (MQ=255)
                                                                   gCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGAGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTccacc                                                                                                 >  5:283649/1‑134 (MQ=255)
                                                                        aGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTc                                                                             <  4:124455/149‑1 (MQ=255)
                                                                          gTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCg                                                                            >  2:133229/1‑148 (MQ=255)
                                                                                atCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCt                                                                     >  5:68959/1‑149 (MQ=255)
                                                                                 gcgtcgtGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGGACACCtt                                                                    <  8:188844/148‑1 (MQ=255)
                                                                                   gtcgtGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTg                                                                   <  5:365137/148‑1 (MQ=255)
                                                                                        gctgctCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCgg                                                             >  8:18543/1‑149 (MQ=255)
                                                                                              ccAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTgcgc                                                       >  1:27670/1‑149 (MQ=255)
                                                                                               cAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTgcgc                                                       <  6:226668/148‑1 (MQ=255)
                                                                                                 ggCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGcgcgcg                                                    <  5:145753/149‑1 (MQ=255)
                                                                                                     cTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCt                                                <  6:151049/149‑1 (MQ=255)
                                                                                                      tCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCtt                                               >  7:379433/1‑149 (MQ=255)
                                                                                                        gATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGa                                             <  3:141335/149‑1 (MQ=255)
                                                                                                        gATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGa                                             <  5:216485/149‑1 (MQ=255)
                                                                                                        gATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGa                                             <  6:68959/149‑1 (MQ=255)
                                                                                                         aTGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGACCACCTTGTCGCGGCTGCGCGCGGTCTTGAg                                            >  6:312885/1‑149 (MQ=255)
                                                                                                          tGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCACGCGGTCTTGAg                                            >  8:141393/1‑148 (MQ=255)
                                                                                                            gCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCAt                                         >  8:6422/1‑149 (MQ=255)
                                                                                                              ttCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGa                                             <  6:270999/143‑1 (MQ=255)
                                                                                                              ttCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGa                                             >  5:270999/1‑143 (MQ=255)
                                                                                                              ttCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGc                                       >  3:385011/1‑149 (MQ=255)
                                                                                                                   gcCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGa                                  >  2:227328/1‑149 (MQ=255)
                                                                                                                            ggCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTg                         <  1:227328/149‑1 (MQ=255)
                                                                                                                              ccGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGa                       <  7:6422/149‑1 (MQ=255)
                                                                                                                                gATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACa                     <  4:385011/149‑1 (MQ=255)
                                                                                                                                gATTTCGACAACGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGc                                           <  7:215713/127‑1 (MQ=255)
                                                                                                                                gATTTCGACAACGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGc                                           >  8:215713/1‑127 (MQ=255)
                                                                                                                                 aTTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAg                    <  1:133229/149‑1 (MQ=255)
                                                                                                                                       aCAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGa              >  8:300962/1‑149 (MQ=255)
                                                                                                                                         aaTGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGAtt            >  6:267267/1‑149 (MQ=255)
                                                                                                                                         aaTGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGAt             >  2:362647/1‑148 (MQ=255)
                                                                                                                                          aTGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTc           <  8:379433/149‑1 (MQ=255)
                                                                                                                                          aTGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCACGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTc           <  7:141393/149‑1 (MQ=255)
                                                                                                                                           cagcgcCCTGGAGCACAAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTc           <  2:359003/146‑1 (MQ=255)
                                                                                                                                           cagcgcCCTGGAGCACAAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTc           >  1:359003/3‑148 (MQ=255)
                                                                                                                                                  cTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCTTTGACGTTCGTTGGACAGCTGCGATTCGACCTtgt   >  4:224333/1‑149 (MQ=255)
                                                                                                                                                   ttGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGACCATGCGTTGACGTTCGTTGGACAGCTGCGATTCGACCTtgtg  >  7:230611/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTCGACCTTGTG  >  NC_002947/4289282‑4289577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑