Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,425,312 T→A 6.4% intergenic (‑370/‑430) PP_5647 ← / → PP_3917 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,425,3120TA 6.4% 187.9 / 3.4 62intergenic (‑370/‑430)PP_5647/PP_3917hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (32/26);  new base A (2/2);  total (34/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

CTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGT  >  NC_002947/4425170‑4425459
                                                                                                                                              |                                                                                                                                                   
cTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGAt                                                                                                                                               >  6:279508/1‑149 (MQ=255)
 tAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATc                                                                                                                                              >  6:90580/1‑149 (MQ=255)
   gACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATc                                                                                                                                              <  7:280766/147‑1 (MQ=255)
        aGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAAtcgtc                                                                                                                                                    <  8:225944/136‑1 (MQ=255)
        aGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAAtcgtc                                                                                                                                                    >  7:225944/1‑136 (MQ=255)
        aGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGa                                                                                                                                       <  2:177026/149‑1 (MQ=255)
           gTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACgg                                                                                                                                    <  5:419758/149‑1 (MQ=255)
            ttCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACggg                                                                                                                                   <  8:10189/149‑1 (MQ=255)
                aTTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCct                                                                                                                               >  6:241477/1‑149 (MQ=255)
                   aCGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTg                                                                                                                                         >  6:178466/1‑136 (MQ=255)
                   aCGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTg                                                                                                                                         <  5:178466/136‑1 (MQ=255)
                     gCTTGGCAGCTACTCCAATGGGCGCTTGTGGTAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTg                                                                                                                           >  2:185766/1‑148 (MQ=255)
                       ttGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGaa                                                                                                                        >  6:36422/1‑149 (MQ=255)
                       ttGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGaa                                                                                                                        >  3:192673/1‑149 (MQ=255)
                          gCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAatc                                                                                                                      <  5:279508/148‑1 (MQ=255)
                             gCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTAGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCg                                                                                                                  >  1:368348/1‑149 (MQ=255)
                                  tCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGGCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTg                                                                                                              >  1:310240/1‑148 (MQ=255)
                                  tCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAatcat                                                                                                                    <  6:204544/142‑1 (MQ=255)
                                  tCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAatcat                                                                                                                    >  5:204544/1‑142 (MQ=255)
                                  tCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAat                                                                                                                       <  2:313740/139‑1 (MQ=255)
                                  tCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAat                                                                                                                       >  1:313740/1‑139 (MQ=255)
                                  tCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTg                                                                                                              >  7:247914/1‑148 (MQ=255)
                                    cAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAAc                                                                                                           >  1:22615/1‑149 (MQ=255)
                                    cAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGAcgc                                                                                                                                                  <  3:132663/110‑1 (MQ=255)
                                    cAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGAcgc                                                                                                                                                  <  3:138638/110‑1 (MQ=255)
                                    cAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGAcgc                                                                                                                                                  >  4:138638/1‑110 (MQ=255)
                                    cAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGAcgc                                                                                                                                                  >  4:132663/1‑110 (MQ=255)
                                              ttGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGTTAGGcc                                                                                                  >  4:317154/1‑148 (MQ=255)
                                                  gATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCg                                                                                             >  3:132772/1‑149 (MQ=255)
                                                             cgcTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATc                                                                                  <  4:192673/149‑1 (MQ=255)
                                                              gcTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCt                                                                                 <  5:90580/149‑1 (MQ=255)
                                                                  ggCATTGGATGCCCTCGATGGGTCCGGTGGGCCTCGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGAc                                                                              <  2:368348/148‑1 (MQ=255)
                                                                    cATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCACGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTg                                                                           >  6:188379/1‑149 (MQ=255)
                                                                              ccTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGc                                                                 >  2:51708/1‑149 (MQ=255)
                                                                              ccTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGc                                                                 <  4:366675/149‑1 (MQ=255)
                                                                                  gATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGtt                                                             <  6:5231/149‑1 (MQ=255)
                                                                                      ggTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATcc                                                         <  4:132772/149‑1 (MQ=255)
                                                                                            gTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTAtct                                                   >  3:302666/1‑149 (MQ=255)
                                                                                               ggCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTg                                                <  1:51708/149‑1 (MQ=255)
                                                                                               ggCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTg                                                <  8:247914/149‑1 (MQ=255)
                                                                                                 ccTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCGAACCGCCATTACGATCGGACTTGGCACTTTGGCCGTTATTCCTATCACTGGt                                              >  8:327948/1‑149 (MQ=255)
                                                                                                 ccTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGt                                              >  3:150430/1‑149 (MQ=255)
                                                                                                  cTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACtt                                                                            <  4:50653/118‑1 (MQ=255)
                                                                                                  cTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACtt                                                                            >  3:50653/1‑118 (MQ=255)
                                                                                                             gcgcCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACctc                                  >  5:289340/1‑149 (MQ=255)
                                                                                                               gcCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACctct                                 >  4:73145/1‑148 (MQ=255)
                                                                                                                 cTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTgg                                               >  5:340409/1‑132 (MQ=255)
                                                                                                                 cTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTgg                                               <  6:340409/132‑1 (MQ=255)
                                                                                                                    gAAAGGGTGGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATTCCTATCTCTGGTCATACGCACCTCTCAATg                            <  7:327948/148‑1 (MQ=255)
                                                                                                                    gAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATg                            <  2:22615/148‑1 (MQ=255)
                                                                                                                         ggTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAg                       >  2:85325/1‑148 (MQ=255)
                                                                                                                          gTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAg                       >  2:374901/1‑147 (MQ=255)
                                                                                                                          gTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGaa                     <  3:317154/149‑1 (MQ=255)
                                                                                                                          gTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCACTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAg                       >  8:186727/1‑147 (MQ=255)
                                                                                                                           tCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAg                    >  2:377036/1‑149 (MQ=255)
                                                                                                                              gTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTcc                 <  1:185766/149‑1 (MQ=255)
                                                                                                                                 aTCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCt                >  2:3232/1‑147 (MQ=255)
                                                                                                                                     gtgtAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGaa                     <  3:292069/138‑1 (MQ=255)
                                                                                                                                     gtgtAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGaa                     >  4:292069/1‑138 (MQ=255)
                                                                                                                                     gtgtAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGGACTTCTGAGcat          <  2:310240/149‑3 (MQ=255)
                                                                                                                                      tgtAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGc                                                                 >  2:237025/1‑93 (MQ=255)
                                                                                                                                      tgtAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGc                                                                 <  1:237025/93‑1 (MQ=255)
                                                                                                                                       gtAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGGACTTCTGAGcatt         <  3:73145/148‑4 (MQ=255)
                                                                                                                                         aaTCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAAtt      >  7:192094/1‑149 (MQ=255)
                                                                                                                                           tcgtcgCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGa    <  5:36422/149‑1 (MQ=255)
                                                                                                                                             gtcgCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAgt  >  7:139773/1‑149 (MQ=255)
                                                                                                                                             gtcgCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAgt  >  5:325713/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
CTAGACTCAGCGTTCAATTACGCTTGGCAGCTACTCCAATGGGCGCTTGTGATAGCTATAGCGCTCGGCATTGGATGCCCTCGATGGGTCCGGTGGGCCTTGTTTCTGCGCGCCTCGAAAGGGTCGGTTATCAGTGTAATCGTCGCGATCCTCTGGACGGGCCCTCTTGGAATCATCGTCTGAACCGGTAGGCCAACCGCCATTACGATCTGACTTGCCACTTTGGCCGTTATCCCTATCTCTGGTCATACGCACCTCTCAATGCTCAGAAGTCCTGAGCTAAATTGAGT  >  NC_002947/4425170‑4425459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑