Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 328,510 A→G 6.6% L247P (CTG→CCG)  PP_0270 ← sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947328,5100AG 6.6% 174.0 / 2.4 60L247P (CTG→CCG) PP_0270sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base A (31/25);  new base G (2/2);  total (33/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

CATCTGCGTGATGTTCTGCTGCAGGCGCTGGCGGTGTTCGTCATCGGGTAAGCGCTCCAGTCGCAGGCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCCGCGTGCGGCATCGGCCAGGCGCCGCAACGGGCGCAGGCACAGGCGCACGGC  >  NC_002947/328365‑328658
                                                                                                                                                 |                                                                                                                                                    
cATCTGCGTGATGTTCTGCTGCAGGCGCTGGCGGTGTTCGTCATCGGGTAAGCGCTCCAGTCGCAGGCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGcc                                                                                                                                                   >  6:351329/1‑149 (MQ=255)
 aTCTGCGTGATGTTCTGCTGCAGGCGCTGGCGGTGTTCGTCATCGGGTAAGCGCTCCAGTCGCAGGCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGCCCATGGCAATCAGCCg                                                                                                                                                  >  4:353445/1‑149 (MQ=255)
    tGCGTGATGTTCTGCTGCAGGCGCTGGCGGTGTTCGTCATCGGGTAAGCGCTCCAGTCGCAGGCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCgctg                                                                                                                                               >  5:38/1‑149 (MQ=255)
         gATGTTCTGCTGCAGGCGCTGGCGGTGTTCGTCATCGGGTAAGCGCTCCAGTCGCAGGCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCgctgct                                                                                                                                             <  4:301048/146‑1 (MQ=255)
         gATGTTCTGCTGCAGGCGCTGGCGGTGTTCGTCATCGGGTAAGCGCTCCAGTCGCAGGCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCgctgct                                                                                                                                             >  3:301048/1‑146 (MQ=255)
          aTGTTCTGCTGCAGGCGCTGGCGGTGTTCGTCATCGGGTAAGCGCTCCAGTCGCAGGCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCa                                                                                                                                          <  2:24185/148‑1 (MQ=255)
           tGTTCTGCTGCAGGCGCTGGCGGTGTTCGTCATCGGGTAAGCGCTCCAGTCGCAGGCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATc                                                                                                                                        <  4:95123/149‑1 (MQ=255)
             ttCTGCTGCAGGCGCTGGCGGTGTTCGTCATCGGGTAAGCGCTCCAGTCGCAGGCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATcgc                                                                                                                                      >  1:134212/1‑149 (MQ=255)
             ttCTGCTGCAGGCGCTGGCGGTGTTCGGCATCGGGTAAGCGCTCCAGTCGCAGGCGCATGCGGGTCAGTGGGGGGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATcgg                                                                                                                                      <  5:342658/149‑2 (MQ=255)
                  ctgcAGGCGCTGGCGGTGTTCGTCATCGGGTAAGCGCTCCAGTCGCAGGCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTg                                                                                                                                  <  5:351329/148‑1 (MQ=255)
                     cAGGCGCTGGCGGTGTTCGTCATCGGGTAAGCGCTCCAGTCGCAGGCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCAAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAAc                                                                                                                               <  2:273421/148‑1 (MQ=255)
                              gCGGTGTTCGTCATCGGGTAAGCGCTCCAGTCGCAGGCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTgcgc                                                                                                                       <  2:138410/147‑1 (MQ=255)
                                  tgtTCGTCATCGGGTAAGCGCTCCAGTCGCAGGCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTg                                                                                                                 >  7:55636/1‑149 (MQ=255)
                                                  aGCGCTCCAGTCGCAGGCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCggg                                                                                                 <  6:38/149‑1 (MQ=255)
                                                     gcTCCAGTCGCAGGCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCa                                                                                              >  1:291465/1‑149 (MQ=255)
                                                        ccAGTCGCAGGCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATc                                                                                            >  7:363918/1‑148 (MQ=255)
                                                           gTCGCAGGCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAgc                                                                                        >  2:148337/1‑149 (MQ=255)
                                                            tCGCAGGCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCt                                                                                                                  >  4:62696/1‑122 (MQ=255)
                                                            tCGCAGGCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCt                                                                                                                  <  3:62696/122‑1 (MQ=255)
                                                              gCAGGCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCGGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAgcgc                                                                                      >  4:339539/1‑148 (MQ=255)
                                                              gCAGGCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCGGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAgcgc                                                                                      >  4:262412/1‑148 (MQ=255)
                                                                 ggCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAgg                                                                                  <  2:134212/149‑1 (MQ=255)
                                                                  gcgcATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAggg                                                                                 >  2:234693/1‑149 (MQ=255)
                                                                     cATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGc                                                                                                                             <  6:252177/102‑1 (MQ=255)
                                                                     cATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGc                                                                                                                             >  5:252177/1‑102 (MQ=255)
                                                                      aTGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCGGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAgg                                                                             <  3:339539/149‑1 (MQ=255)
                                                                      aTGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCGGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAgg                                                                             <  3:262412/149‑1 (MQ=255)
                                                                        gCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGgct                                                                           >  7:80633/1‑149 (MQ=255)
                                                                          gggTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGgctgc                                                                         >  4:120698/1‑149 (MQ=255)
                                                                          gggTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGgctgc                                                                         >  2:96917/1‑149 (MQ=255)
                                                                             tCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTc                                                                       >  5:339834/1‑148 (MQ=255)
                                                                                gTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAgg                                                                   >  7:222219/1‑149 (MQ=255)
                                                                                gTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAgg                                                                   <  4:202770/149‑1 (MQ=255)
                                                                                           aGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCgg                                                                                                  >  1:162138/1‑107 (MQ=255)
                                                                                           aGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAg                                                        <  1:148337/149‑1 (MQ=255)
                                                                                            ggccATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAgg                                                       >  6:5666/4‑149 (MQ=255)
                                                                                                 tGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCcgcg                                                  >  8:246238/1‑149 (MQ=255)
                                                                                                 tGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGAGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCcgcg                                                  >  4:361053/1‑149 (MQ=255)
                                                                                                   agaCACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGTCGCGt                                                 <  8:55636/148‑1 (MQ=255)
                                                                                                       acGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCCGCGTGCgg                                             >  3:240259/1‑148 (MQ=255)
                                                                                                       acGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCCGCGTGCGGc                                            <  8:80633/149‑1 (MQ=255)
                                                                                                         ggcggcCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCCGCGTGCGGCAt                                          >  5:282195/1‑149 (MQ=255)
                                                                                                          gcggcCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCCGCGTGCGGCATc                                         >  2:82759/1‑149 (MQ=255)
                                                                                                               cAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCCGCGTGCGGCATCGGCCa                                    <  6:339834/149‑1 (MQ=255)
                                                                                                                aGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCCGCGTGCGGCATCGGCCAg                                   >  3:237220/1‑149 (MQ=255)
                                                                                                                  gAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCCGCGTGCGGCATCGGCCAGgc                                 <  5:5666/149‑1 (MQ=255)
                                                                                                                      tAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCCGCGTGCGGCATCGGCCAGGcgccg                             <  8:222219/149‑1 (MQ=255)
                                                                                                                        ggCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCCGCGTGCGGCATCGGCCAGgcgc                               <  8:283384/145‑1 (MQ=255)
                                                                                                                        ggCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCCGCGTGCGGCATCGGCCAGgcgc                               >  7:283384/1‑145 (MQ=255)
                                                                                                                          ccTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCCGCGTGCGGCATCGGCCAGGCGCCGCAAc                         <  3:353445/149‑1 (MQ=255)
                                                                                                                                 gTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCCGCGTGCGGCATCGGCCAGGCGCCGCAACGGgcg                    >  8:363394/1‑147 (MQ=255)
                                                                                                                                  ttGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCCGCGTGCGGCATCGGCCAGGCGCCGCAACGGGCGCAg                 <  7:363394/149‑1 (MQ=255)
                                                                                                                                  ttGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCCGCGTGCGGCATCGGCCAGGCGCCGCAACGGGCGCAg                 <  2:291465/149‑1 (MQ=255)
                                                                                                                                       cATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCCGCGTGCGGCATCGGCCAGGCGCCGCAACGGGCGCAGGca              >  6:85391/1‑147 (MQ=255)
                                                                                                                                        aTGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCCGCGTGCGGCATCGGCCAGGCGCCGCAACGGGCGCAGGCACAg           <  3:361053/149‑1 (MQ=255)
                                                                                                                                        aTGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCCGCGTGCGGCATCGGCCAGGCGCCGCAACGGGCGCAGGCACAg           >  4:362884/1‑149 (MQ=255)
                                                                                                                                          ggCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCCGCGTGCGGCATCGGCCAGGCGCCGCAACGGGCGCAGGCACAGgc         >  2:282441/1‑149 (MQ=255)
                                                                                                                                           gCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCCGCGTGCGGCATCGGCCAGGCGCCGCAACGGGCGCAGGCACAGgc         <  4:240259/148‑1 (MQ=255)
                                                                                                                                             aaTCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGgctgc                                                                         <  7:322035/82‑1 (MQ=255)
                                                                                                                                             aaTCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGgctgc                                                                         >  8:322035/1‑82 (MQ=255)
                                                                                                                                                 aGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCCGCGTGCGGCATCGGCCAGGCGCCGCAACGGGCGCAGGCACAGGCGCACGGc  <  8:363918/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CATCTGCGTGATGTTCTGCTGCAGGCGCTGGCGGTGTTCGTCATCGGGTAAGCGCTCCAGTCGCAGGCGCATGCGGGTCAGTGGGGTGCGCAGGTCATGAGACACGGCGGCCAGGAAGTAGGCCTTGTCGTTGACCATGGCAATCAGCCGCTGCTGCATCGCGTTGAACGCCTGCGCCGCCTGGCGCACTTCCTCCGGGCCATCCAGCGCCAGGGGAGGCTGCTCGAGGTTGCTGCCCAGGCCGCGTGCGGCATCGGCCAGGCGCCGCAACGGGCGCAGGCACAGGCGCACGGC  >  NC_002947/328365‑328658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑