Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,478,868 G→C 9.0% V73V (GTG→GTC)  ybdR → Zn‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,478,8680GC 9.0% 163.2 / 4.3 55V73V (GTG→GTC) ybdRZn‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (22/28);  new base C (2/3);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGACGACATCATCTTAAGGGTGACGGCCACCGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGGCGCGGATCGATCATCAACAAAAAGGGCATACCACCA  >  NC_002947/4478722‑4479015
                                                                                                                                                  |                                                                                                                                                   
gCAGACGACATCATCTTAAGGGTGACGGCCACCGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTgac                                                                                                                                                   <  1:45776/149‑1 (MQ=255)
   gacgacATCATCTTAAGGGTGACGGCCACCGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTgacgac                                                                                                                                                >  2:191306/1‑149 (MQ=255)
         atcatcTTAAGGGTGACGGCCACCGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTg                                                                                                                                            >  1:283467/1‑147 (MQ=255)
            atcTTAAGGGTGACGGCCACCGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGTCGTCACGTCCCTGccaa                                                                                                                                        <  8:220219/148‑4 (MQ=255)
            atcTTAAGGGTGACGGCCACCGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAgg                                                                                                                                        <  3:207853/148‑1 (MQ=255)
                 aaGGGTGACGGCCACCGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTg                                                                                                                                     <  1:280329/146‑1 (MQ=255)
                  aGGGTGACGGCCACCGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGa                                                                                                                                 <  4:348831/149‑1 (MQ=255)
                        aCGGCCACCGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAgt                                                                                                                           <  2:283467/149‑1 (MQ=255)
                          ggCCACCGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGAcc                                                                                                                               <  3:152445/143‑1 (MQ=255)
                          ggCCACCGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGAcc                                                                                                                               >  4:152445/1‑143 (MQ=255)
                          ggCCACCGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAgtgg                                                                                                                         >  3:122155/1‑149 (MQ=255)
                                cGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGTCGTCACGTCCCTGccaa                                                                                                                                        >  6:197001/1‑125 (MQ=255)
                                cGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGTCGTCACGTCCCTGccaa                                                                                                                                        <  5:197001/128‑4 (MQ=255)
                                     tCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCtttt                                                                                                              >  6:396653/1‑149 (MQ=255)
                                         cGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCtttt                                                                                                              <  6:418174/145‑1 (MQ=255)
                                         cGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCtttt                                                                                                              <  6:328843/145‑1 (MQ=255)
                                               cGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCtt                                                                                                    >  5:185659/1‑149 (MQ=255)
                                                gACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGTACGTGACGACCCTGCAGGTTGGCGACCGAGTGTTGATTACATTGGTCATCGCTtg                                                                                                   >  6:236266/1‑149 (MQ=255)
                                                  ccTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTtgtg                                                                                                 <  4:122155/149‑1 (MQ=255)
                                                     ccACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTgg                                                                                                >  7:215067/1‑147 (MQ=255)
                                                     ccACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGaa                                                                                              >  7:209364/1‑149 (MQ=255)
                                                              tCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCtttt                                                                                     >  7:189379/1‑149 (MQ=255)
                                                                   gCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTc                                                                                   >  1:29666/1‑146 (MQ=255)
                                                                   gCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTc                                                                                   <  2:29666/146‑1 (MQ=255)
                                                                   gCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCttc                                                                                >  4:258/1‑147 (MQ=255)
                                                                   gCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGc                                                                                <  8:189379/149‑1 (MQ=255)
                                                                    cAAGATCCCGGAAACAGAGCAAGGCGACTTCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGc                                                                                >  1:231362/1‑148 (MQ=255)
                                                                     aaGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGc                                                                                >  8:364759/1‑147 (MQ=255)
                                                                       gATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGGTTGTGGATTTTGGTTTTTCTGCcag                                                                             >  4:274397/1‑148 (MQ=255)
                                                                            cGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTgg                                                                                                <  8:79584/124‑1 (MQ=255)
                                                                            cGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGAt                                                                       <  2:293596/149‑1 (MQ=255)
                                                                              gAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGAtct                                                                     <  5:356936/149‑1 (MQ=255)
                                                                               aaaCAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGAtctc                                                                    <  6:185659/149‑1 (MQ=255)
                                                                                  cAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTAGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTc                                                                 >  1:310598/1‑149 (MQ=255)
                                                                                   agagCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCg                                                                <  1:66352/149‑1 (MQ=255)
                                                                                     agCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCTTTGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCGTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCt                                                              <  4:344344/149‑1 (MQ=255)
                                                                                     agCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGCCCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTATTCGCt                                                              >  5:324913/1‑149 (MQ=255)
                                                                                           gCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCtgt                                                        <  5:396653/149‑1 (MQ=255)
                                                                                              aCATCTTCGGCCACGAGTTCATGGGCATCTTGGAAGAGGTTGGCGGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGaa                                                     <  7:353032/149‑1 (MQ=255)
                                                                                                atgtttggCCACGTGTTCATGGGCTTCGTGGAAGAGGTTGGCATGGTCGTGACGTCCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAac                                                   <  5:236266/143‑1 (MQ=255)
                                                                                                     cGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAaccac                                                <  1:292260/147‑1 (MQ=255)
                                                                                                     cGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAaccac                                                <  8:215067/147‑1 (MQ=255)
                                                                                                     cGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAaccac                                                <  8:209364/147‑1 (MQ=255)
                                                                                                            gAGTTTATGGGCATCGTGGAAGATGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCgg                                       <  7:364759/149‑1 (MQ=255)
                                                                                                            gAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCgg                                       >  3:239107/1‑149 (MQ=255)
                                                                                                              gTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCtgt                                                        <  5:379823/130‑1 (MQ=255)
                                                                                                              gTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCtgt                                                        >  6:379823/1‑130 (MQ=255)
                                                                                                                 cATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGgcgc                                   <  7:100573/148‑1 (MQ=255)
                                                                                                                          cGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGGCGCGgatcgatc                          >  7:58558/1‑148 (MQ=255)
                                                                                                                              gAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGCGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGGCGCGGATCGATCATcaa                     >  7:28009/1‑149 (MQ=255)
                                                                                                                                 ccaacctgCAGGGTCGTCACGTCCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGGCGCGGATCGatcat                        <  3:224102/136‑1 (MQ=255)
                                                                                                                                 ccaacctgCAGGGTCGTCACGTCCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGGCGCGGATCGatcat                        >  4:224102/8‑143 (MQ=255)
                                                                                                                                        gCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGGCGCGGATCGATCATCAACAAAAAGGGc           <  2:231362/149‑1 (MQ=255)
                                                                                                                                        gCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGCGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGGCGCGGATCGATCATCAACAAAAAGGGc           <  8:28009/149‑1 (MQ=255)
                                                                                                                                          aGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGGCGCGGATCGATCATCAACAAAAAGGGCa          >  7:185478/1‑148 (MQ=255)
                                                                                                                                               cGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGGCGCGGATCGATCATCAACAAAAAGGGCATaccac    >  6:205724/1‑149 (MQ=255)
                                                                                                                                                 tGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGGCGCGGATCGATCATCAACAAAAAGGGCATAccacca  <  2:108724/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GCAGACGACATCATCTTAAGGGTGACGGCCACCGCCATCTGCGGATCCGACCTCCACCTCTATCGAGGCAAGATCCCGGAAACAGAGCAAGGCGACATCTTCGGCCACGAGTTCATGGGCATCGTGGAAGAGGTTGGCAGGGACGTGACGACCCTGCAGGTTGGCGACCGAGTGGTGATTCCTTTTGTCATCGCTTGTGGAAGTTGCTTTTTCTGCCAGCAGGATCTCTTCGCTGCCTGTGAAACCACCAATACCGGGCGCGGATCGATCATCAACAAAAAGGGCATACCACCA  >  NC_002947/4478722‑4479015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑