Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,503,790	A→G	15.9%	intergenic (+39/‑59)	xanP → / → tusD	xanthine permease/sulfur transfer protein complex subunit TusD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,503,790	0	A	G	15.9%	51.2 / 2.5	26	intergenic (+39/‑59)	xanP/tusD	xanthine permease/sulfur transfer protein complex subunit TusD
Reads supporting (aligned to +/- strand):  ref base A (12/9);  new base G (2/2);  total (15/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCCGGCGGCGATTCGCAATGTGCTGGAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCTGGCCGGCGGG  >  NC_002947/4503647‑4503938                                                                                                                                                |                                                                                                                                                     aTGCCGGCGGCGATTCGCAATGTGCTGGAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTATCCCGCGCCCGCTTGCACAAgc                                                                                                                                                 >  6:231056/1‑149 (MQ=255)    ccGGCGGCGATTCGCAATGTGCTGGAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAgcgg                                                                                                                                               <  4:163641/148‑1 (MQ=255)       gcggcgATTCGCAATGTGCTGGAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGTGCAAgcgggcgc                                                                                                                                           <  7:130093/149‑1 (MQ=255)        cggcgATTCGCAATGTGCTGGAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAgcgggcgcg                                                                                                                                          <  3:343200/149‑1 (MQ=255)                            gAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATg                                                                                                                      >  3:328958/1‑149 (MQ=255)                            gAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCCCGCCCGCATGCCCCAGCGGGCGCGGGCTTTTTTGGTTTATCATg                                                                                                                      >  6:226531/1‑149 (MQ=255)                               tggggTGTGGCTACCGGGTTGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGc                                                                                                                    <  5:226531/146‑1 (MQ=255)                                  ggTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATc                                                                                                                         >  3:269337/1‑140 (MQ=255)                                  ggTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATc                                                                                                                         <  4:269337/140‑1 (MQ=255)                                                                  gCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCGTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCt                                                                                >  4:307372/1‑149 (MQ=255)                                                                                                      ttGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATAATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTcg                                            >  5:409378/1‑149 (MQ=255)                                                                                                          aCCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGAc                                        <  4:328958/149‑1 (MQ=255)                                                                                                                    tGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCt                                 <  6:299419/146‑1 (MQ=255)                                                                                                                    tGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTgag                              >  5:299419/1‑147 (MQ=255)                                                                                                                        tGAGAGTATCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTc                          <  5:231056/149‑1 (MQ=255)                                                                                                                                  ccgcgcccgcTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCgg                >  5:41557/1‑149 (MQ=255)                                                                                                                                   cgcgcccgcTTGTGCAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTGCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCg                 <  5:391944/147‑1 (MQ=255)                                                                                                                                   cgcgcccgcTTGTGCAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGAACCATTCCGTTGCATGAGGTAGCCATGAAATTCGCTTTCGCGGGGTTCTCCCCGGCCCCGGCGCCCTCTTCGCGACGCGCCCGGCGCGGCGCCGAGGCg                 >  6:391944/1‑147 (MQ=255)                                                                                                                                   cgcgcccgcTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGt               >  6:162244/1‑149 (MQ=255)                                                                                                                                   cgcgcccgcTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGt               >  8:47116/1‑149 (MQ=255)                                                                                                                                      gcccgcTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCt            >  6:208119/1‑149 (MQ=255)                                                                                                                                        ccgcTTGTGCAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATGGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCTgg          >  8:344854/1‑149 (MQ=255)                                                                                                                                        ccgcTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCTgg          >  6:8377/1‑149 (MQ=255)                                                                                                                                            ttGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGa                     >  4:382381/1‑134 (MQ=255)                                                                                                                                            ttGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGa                     <  3:382381/134‑1 (MQ=255)                                                                                                                                              gCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCTGGCcgg      <  5:208119/147‑1 (MQ=255)                                                                                                                                               cacaAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCTGGCcggcg    <  5:8377/148‑1 (MQ=255)                                                                                                                                                acaAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCTGGCCGGCggg  >  5:47498/1‑149 (MQ=255)                                                                                                                                                acaAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCTGGCCGGCggg  >  1:249404/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                     ATGCCGGCGGCGATTCGCAATGTGCTGGAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCGCGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCTGGCCGGCGGG  >  NC_002947/4503647‑4503938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑