Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,522,241 C→G 6.2% F30L (TTC→TTG) ‡ idh → isocitrate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,522,2410CG 6.2% 305.6 / 7.5 96F30L (TTC→TTG) ‡idhisocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (41/49);  new base G (3/3);  total (44/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

CGCCTACCCTGCGCACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTACCACCCCTGAAGC  >  NC_002947/4522094‑4522387
                                                                                                                                                   |                                                                                                                                                  
cgcCTACCCTGCGCACCACGAGACTCGAGCACGCTCAGCAGACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTc                                                                                                                                                    <  5:354147/148‑1 (MQ=255)
 gcCTACCCTGCGCACCACGAGACGCGATCACGCTCAGCACCCAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGGGTTCAc                                                                                                                                                  <  4:209152/149‑1 (MQ=255)
           cgcACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGc                                                                                                                                         <  1:169531/148‑1 (MQ=255)
           cgcACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCt                                                                                                                                        <  3:293966/149‑1 (MQ=255)
           cgcACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGGTGAACGCTTCga                                                                                                                                          <  6:201609/147‑2 (MQ=255)
            gcACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTg                                                                                                                                       >  1:251822/1‑149 (MQ=255)
             caccacGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGa                                                                                                                                      <  4:283881/149‑1 (MQ=255)
                cacGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACAt                                                                                                                                   <  3:143785/149‑1 (MQ=255)
                cacGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACAt                                                                                                                                   >  3:324810/1‑149 (MQ=255)
                      aCTCGAGCACTCGCAGAACACAGAGAGTTAACCCTCCTGCCCACACGTGCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGt                                                                                                                             <  4:221149/149‑1 (MQ=255)
                      aCTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCCTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGt                                                                                                                             <  4:341712/149‑1 (MQ=255)
                      aCTCGAGCACGCTCAGCACACAGAGAGTTAACCCCCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGt                                                                                                                             <  2:368720/149‑1 (MQ=255)
                        tCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTc                                                                                                                            >  7:195728/1‑148 (MQ=255)
                          gAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCCCTGTCGa                                                                                                                          >  3:140532/1‑148 (MQ=255)
                           aGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGaaa                                                                                                                        <  6:394481/149‑1 (MQ=255)
                                gCTCAGCACACAGAGAGGTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTCCTCGCTGCTGCCGATCATCGACGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTcgc                                                                                                                   <  6:137402/149‑1 (MQ=255)
                                  tCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGa                                                                                                                 >  7:203460/1‑149 (MQ=255)
                                    aGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACa                                                                                                               >  3:62460/1‑149 (MQ=255)
                                       acacaGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACAttt                                                                                                            <  4:324810/149‑1 (MQ=255)
                                       acacaGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACAttt                                                                                                            <  5:303111/149‑1 (MQ=255)
                                         acaGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTcc                                                                                                          <  4:62460/149‑1 (MQ=255)
                                            gagagTTAACCCGCATGCCCACCCGTTCAAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCctg                                                                                                       >  1:23481/1‑149 (MQ=255)
                                                 ttAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCctggctgg                                                                                                  >  1:366619/1‑149 (MQ=255)
                                                 ttAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCctggctgg                                                                                                  >  5:120376/1‑149 (MQ=255)
                                                  tAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACa                                                                                                               <  8:3501/135‑1 (MQ=255)
                                                  tAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACa                                                                                                               >  7:3501/1‑135 (MQ=255)
                                                    aCCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGcc                                                                                                <  2:251822/148‑1 (MQ=255)
                                                     cccGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCg                                                                                               >  3:373130/1‑148 (MQ=255)
                                                        gCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATc                                                                                           >  5:113305/1‑149 (MQ=255)
                                                            gCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGcc                                                                                                >  5:213376/1‑140 (MQ=255)
                                                            gCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGcc                                                                                                <  6:213376/140‑1 (MQ=255)
                                                            gCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTg                                                                                       >  8:233648/1‑149 (MQ=255)
                                                              ccACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTgcc                                                                                     <  7:62594/149‑1 (MQ=255)
                                                              ccACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGGTGAACGCTTCga                                                                                                                                          >  2:172812/1‑95 (MQ=255)
                                                              ccACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGGTGAACGCTTCga                                                                                                                                          <  1:172812/96‑2 (MQ=255)
                                                               gctgtgTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTgccg                                                                                    <  4:336475/144‑1 (MQ=255)
                                                                  ccGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCct                                                                                                        >  6:117549/1‑126 (MQ=255)
                                                                  ccGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCt                                                                                 <  7:233648/149‑1 (MQ=255)
                                                                  ccGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCt                                                                                 >  1:113675/1‑149 (MQ=255)
                                                                         aaGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGa                                                                          <  2:366619/149‑1 (MQ=255)
                                                                         aaGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGa                                                                          <  3:262337/149‑1 (MQ=255)
                                                                          atatcatgTATAGCTTGACCGACGAAGGCCCGGCCGTCGGCACTTACTCGCTGCTGGCGATCATCGAAGCGTTCTGCGCTTCGGCTGACTTCGCTGTCGAAACTCGCGACATTTGCCTGGCTGGCCGTATCCTTGCCGGCTTCGCGGAg                                                                         <  4:115847/147‑1 (MQ=255)
                                                                               atcTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACt                                                                    <  5:326642/149‑1 (MQ=255)
                                                                                  tataCCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTggg                                                                 >  6:132048/1‑149 (MQ=255)
                                                                                   ataCCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTc                                                                               >  1:249825/1‑134 (MQ=255)
                                                                                   ataCCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTc                                                                               <  2:249825/134‑1 (MQ=255)
                                                                                   ataCCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGgc                                                                >  4:91454/1‑149 (MQ=255)
                                                                                    taCCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTgg                                                                  <  6:120376/146‑1 (MQ=255)
                                                                                    taCCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTgg                                                                  <  4:140532/146‑1 (MQ=255)
                                                                                       cTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCg                                                                            <  6:137886/133‑1 (MQ=255)
                                                                                       cTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGgcg                                                               >  3:71891/1‑146 (MQ=255)
                                                                                       cTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGgcg                                                               >  5:306136/1‑146 (MQ=255)
                                                                                       cTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCa                                                             <  4:71891/148‑1 (MQ=255)
                                                                                       cTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTACCGCCTTCCCGGAGCAACTGGgcg                                                               >  1:92289/1‑146 (MQ=255)
                                                                                           aCCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCagag                                                          >  5:34813/1‑147 (MQ=255)
                                                                                            ccGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAg                                                       >  2:21613/1‑149 (MQ=255)
                                                                                              gacgaAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATc                                                                                           >  6:185969/1‑111 (MQ=255)
                                                                                              gacgaAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATc                                                                                           <  5:185969/111‑1 (MQ=255)
                                                                                               tcgaAGCCCACGCCGTCGCCAGTTACTCGCTGCTGCCTATCATCGAAGCGTTCACCGCTTCGGCTTACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTTTGGTTGCCGCCTTCCCGGAGCAACTGGGCGGAGAGAAGc                                                      <  5:190188/146‑1 (MQ=255)
                                                                                               acgaAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTACCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCaa                                                    <  2:92289/149‑1 (MQ=255)
                                                                                                 gaAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGt                                                  <  6:113305/149‑1 (MQ=255)
                                                                                                 gaAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGt                                                  <  3:91454/149‑1 (MQ=255)
                                                                                                       cccGCCCTCGCCACTTACTCGCTGCTTGCGATCATGGAAGCGTTCAGCGCTTCGGCTGACTTCGCTGTGGAAACTCGCGAGATTTCGCTGTCTGGCAGTTTGCTTGCCGCCTTACCGGAGCAACTTGGCGCAGAGAAGCATGTTGGCGa                                            <  8:203460/149‑1 (MQ=255)
                                                                                                       cccGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGa                                            >  7:248051/1‑149 (MQ=255)
                                                                                                       cccGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCGTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCGTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGa                                            >  1:300407/1‑149 (MQ=255)
                                                                                                            ccTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCAcc                                       >  5:78887/1‑149 (MQ=255)
                                                                                                             cTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTCTCGAAACTCGCGACATTTCACTGGCTGGCCGTATACTTGCCGCCTTACCGGAGAAAATGGGCGCAGAGAAGCAAGTAGGCGATCAACt                                      >  1:111445/1‑149 (MQ=255)
                                                                                                              tCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTg                                     >  5:301740/1‑149 (MQ=255)
                                                                                                                gCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTTGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGc                                   <  3:6596/149‑1 (MQ=255)
                                                                                                                gCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGc                                   <  1:239694/149‑1 (MQ=255)
                                                                                                                  cACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGa                                            <  2:96963/138‑1 (MQ=255)
                                                                                                                  cACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGa                                            >  1:96963/1‑138 (MQ=255)
                                                                                                                     ttACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAAc                              >  2:318208/1‑149 (MQ=255)
                                                                                                                        cTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTgg                           <  1:318208/149‑1 (MQ=255)
                                                                                                                           gctgctGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCg                                             <  3:4272/128‑1 (MQ=255)
                                                                                                                           gctgctGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCg                                             >  4:4272/1‑128 (MQ=255)
                                                                                                                           gctgctGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGa                                            <  4:274210/129‑1 (MQ=255)
                                                                                                                           gctgctGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGa                                            >  3:274210/1‑129 (MQ=255)
                                                                                                                           gctgctGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTggg                          <  4:373130/147‑1 (MQ=255)
                                                                                                                                 gcCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTgg                  >  8:216181/1‑149 (MQ=255)
                                                                                                                                  ccGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTgg                  <  6:34813/148‑1 (MQ=255)
                                                                                                                                  ccGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGc                 >  7:367257/1‑149 (MQ=255)
                                                                                                                                  ccGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGc                 >  3:235600/1‑149 (MQ=255)
                                                                                                                                  ccGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGc                 <  5:132048/149‑1 (MQ=255)
                                                                                                                                     tcagccGAAGCGGTGAACGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCag                                                            <  4:8507/98‑1 (MQ=255)
                                                                                                                                     tcagccGAAGCGGTGAACGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCag                                                            >  3:8507/6‑103 (MQ=255)
                                                                                                                                         ccGAAGCGGTGAACGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTCGGCCAGCTGGCTaccgcc          >  5:348316/2‑149 (MQ=255)
                                                                                                                                           gAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTACCAccc         <  7:142888/148‑1 (MQ=255)
                                                                                                                                           gAAGCGTGCACCGCTTCGGCTGACATCGCTGGCGCAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTACCAccc         <  2:300407/148‑1 (MQ=255)
                                                                                                                                            aaGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTACCACCCCt       <  1:21613/149‑1 (MQ=255)
                                                                                                                                            aaGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACAGGGCGCAGAGAAGCAAGTAGGCGATCACCTGCCGGAACTCGGCCAGCTGGCTACCACACCt       >  4:64494/1‑149 (MQ=255)
                                                                                                                                             aGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTgg                  <  6:317082/137‑1 (MQ=255)
                                                                                                                                             aGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTgg                  >  5:317082/1‑137 (MQ=255)
                                                                                                                                               cGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTACCACCCCTGaa    <  6:78887/149‑1 (MQ=255)
                                                                                                                                               cGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCCGGCGGAACTGGGCCAGCTGGCTACCACCCCTGaa    <  6:306136/149‑1 (MQ=255)
                                                                                                                                                gTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGAGGAACTGGGCCAGCTGGCTACCACCCCTGAAg   >  5:106360/1‑149 (MQ=255)
                                                                                                                                                 ttCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTACCACCCCTGAAGc  >  4:376113/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGCCTACCCTGCGCACCACGAGACTCGAGCACGCTCAGCACACAGAGAGTTAACCCGCATGCCCACCCGTTCCAAGATCATCTATACCTTCACCGACGAAGCCCCCGCCCTCGCCACTTACTCGCTGCTGCCGATCATCGAAGCGTTCACCGCTTCGGCTGACATCGCTGTCGAAACTCGCGACATTTCCCTGGCTGGCCGTATCCTTGCCGCCTTCCCGGAGCAACTGGGCGCAGAGAAGCAAGTAGGCGATCACCTGGCGGAACTGGGCCAGCTGGCTACCACCCCTGAAGC  >  NC_002947/4522094‑4522387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑