| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,547,602 | A→C | 10.4% | intergenic (‑257/‑87) | pydP ← / → pydB | NCS1 family transporter PydP/bifunctional D‑hydantoinase/dihydropyrimidinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,547,602 | 0 | A | C | 10.4% | 157.3 / 5.4 | 57 | intergenic (‑257/‑87) | pydP/pydB | NCS1 family transporter PydP/bifunctional D‑hydantoinase/dihydropyrimidinase |
| Reads supporting (aligned to +/- strand): ref base A (26/25); new base C (3/3); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
AGTCAAATACCAGGAAAACCCCGGCGCCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTACCCC > NC_002947/4547461‑4547742 | aGTCAAATACCAGGAAAACCCCGGCGCCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACa < 3:42893/148‑1 (MQ=255) tCAAATACCAGGAAAACCCCGGCGCCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGa < 6:245706/149‑1 (MQ=255) tCAAATACCAGGAAAACCCCGGCGCCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGa > 3:170899/1‑149 (MQ=255) cAAATACCAGGAAAACCCCGGCGCCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGAt > 1:372990/1‑149 (MQ=255) cAAATACCAGGAAAACCCCGGCAACCATACGCCCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAAAGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTCTCAGGACTGACAGGaa > 6:336660/1‑148 (MQ=255) tACCAGGAAAACCCCCGCGCCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTAAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGTCCTGACAAGAttcga < 8:139507/149‑4 (MQ=255) ccAGGAAAACCCCGGCGCCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGAccg > 3:77952/1‑149 (MQ=255) ccAGGAAAACCCCGGCGCCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCCTGTCAGTCCTGACAAGAttcgag < 1:51275/148‑5 (MQ=255) aGGAAAACCCCGGCGCCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCCTGTCAGTCCTGACAAGAttcga < 8:222522/145‑4 (MQ=255) aGGAAAACCCCGGCGCCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCCTGTCAGTCCTGACAAGAttcga > 7:222522/1‑142 (MQ=255) aaaCCCCGGCGCCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGAccgccctgc > 3:202980/1‑149 (MQ=255) ccccCGCGACCATGGGCGCGTTAAATTTTGCATTTTTATATCAACAATTTAAAAACCACAAATTTAATGGTAAAATTTTTTTGCTTAATCCAGAATCAGCATCTAGCCTCGATTATTGTCAGGACTGACAGTATTGAccgccattcccg < 8:311825/149‑1 (MQ=255) aacGGCGCCCATTCTCGCCTTAATTATTAACTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTACTTGCTGAATCCAGAATCAGCATCTAGCATCGAATCTATTAAGTAATGACACGATTGAacgccctcaccgc > 4:75555/3‑149 (MQ=255) ccGGCGCCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGAccgccctgcccgcc < 7:51289/149‑1 (MQ=255) gcgcCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACa > 2:174872/1‑125 (MQ=255) gcgcCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACa < 1:174872/125‑1 (MQ=255) cgcCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGAccgccctgcccgccctgc < 7:335763/149‑1 (MQ=255) agcCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGAccgccctgcccgccctg > 4:52559/2‑148 (MQ=255) gcCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCa > 6:103371/1‑149 (MQ=255) gcgcgTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGa > 2:68892/1‑148 (MQ=255) gcgcgTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGa > 8:13622/1‑148 (MQ=255) gatAATTTTTTACTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATg < 8:375945/147‑1 (MQ=255) ttttttCCTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCa > 6:415425/1‑146 (MQ=255) tttCCTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAAc > 5:33412/1‑149 (MQ=255) ttCCTATTTATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAAc < 7:333822/148‑1 (MQ=255) ccTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCg < 1:68892/148‑1 (MQ=255) cTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGc < 4:77952/149‑1 (MQ=255) ttGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCa < 4:170899/148‑1 (MQ=255) acaacTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGt > 1:403574/1‑147 (MQ=255) ttAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTg > 7:283758/1‑149 (MQ=255) aaaaaCCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGc < 3:52559/148‑1 (MQ=255) aaaaCCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCCAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCg < 5:415425/148‑1 (MQ=255) tttAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCa < 8:25947/149‑1 (MQ=255) tttAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCa < 8:20064/149‑1 (MQ=255) aTGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTc > 1:11646/1‑149 (MQ=255) ttttCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGACCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGAt > 4:149260/1‑149 (MQ=255) cTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGgcg > 4:208082/1‑149 (MQ=255) cTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGCACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGgc < 8:106387/148‑1 (MQ=255) aaTCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCa > 6:189902/1‑149 (MQ=255) tCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATTACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTGGATCCGTGGCGCCAcc > 2:301583/1‑149 (MQ=255) tCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTTTTGATCCGTGGCGCCAcc < 4:202980/149‑1 (MQ=255) tCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGACCGCCCTGCATCCATGACAATGCAAAGAACCGGCCTAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCAcc > 6:409144/1‑149 (MQ=255) ccAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTg < 5:341032/140‑1 (MQ=255) ccAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTg > 6:341032/1‑140 (MQ=255) aGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTg < 6:33412/149‑1 (MQ=255) tCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTa > 6:174296/1‑149 (MQ=255) aTCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCAc < 7:13622/149‑1 (MQ=255) gCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGa < 3:208082/146‑1 (MQ=255) ccTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAagag < 3:75555/149‑1 (MQ=255) cTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAagaga < 2:372990/149‑1 (MQ=255) cTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAagaga < 8:283758/149‑1 (MQ=255) gtcaaTCCTGTCAGTCCTGACAAGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAagagag > 6:240097/4‑149 (MQ=255) gtcaaTCCTGTCAGTCCTGACAAGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAagagag > 8:345469/4‑149 (MQ=255) aTCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTAc < 2:11646/149‑1 (MQ=255) tCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTAcc > 7:317712/1‑149 (MQ=255) tCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTAcc > 1:40325/1‑149 (MQ=255) ttGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTAcccc > 3:305702/1‑149 (MQ=255) | AGTCAAATACCAGGAAAACCCCGGCGCCCATTCGCGCGTTAATTTTTTCCTATTGATATCAACAACTTAAAAACCACAAATTTAATGGGAAAATTTTCTTGCTGAATCCAGAATCAGCATCTAGCCTCGAATCTTGTCAGGACTGACAGGATTGACCGCCCTGCCCGCCCTGCATCCATGACAATGCAAAGAACCGGCCAAGACCGGTCAGCCTGCGAGGAAAACGGCATGTCCCTGTTGATCCGTGGCGCCACCGTGGTTACCCACGAAGAGAGTTACCCC > NC_002947/4547461‑4547742 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |