Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,549,025 A→C 5.8% E446A (GAG→GCG)  pydB → bifunctional D‑hydantoinase/dihydropyrimidinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,549,0250AC 5.8% 210.0 / 3.3 69E446A (GAG→GCG) pydBbifunctional D‑hydantoinase/dihydropyrimidinase
Reads supporting (aligned to +/- strand):  ref base A (34/31);  new base C (2/2);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

ACCCGCAGGGCACCCGCACCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGC  >  NC_002947/4548878‑4549163
                                                                                                                                                   |                                                                                                                                          
aCCCGCAGGGCACCCGCACCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAg                                                                                                                                           <  6:160383/149‑1 (MQ=255)
 cccGCAGGGCACCCGCACCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGc                                                                                                                                          <  1:206135/149‑1 (MQ=255)
  ccGCAGGGCACCCGCACCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGcc                                                                                                                                         >  6:90194/1‑149 (MQ=255)
  ccGCAGGGCACCCGCACCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGcc                                                                                                                                         >  4:353137/1‑149 (MQ=255)
  ccGCAGGGCACCCGCACCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGcc                                                                                                                                         >  3:268182/1‑149 (MQ=255)
        ggCACCCGCACCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGgcgc                                                                                                                                   >  6:360379/1‑149 (MQ=255)
        ggCACCCGCACCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCCCCGCGCCCGgctc                                                                                                                                   >  1:39816/1‑147 (MQ=255)
         gCACCCGCACCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGcgcg                                                                                                                                  >  8:176787/1‑149 (MQ=255)
             ccGCACCATCTCCGCCAAAACCCACCACCAGCAGGAGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGgc                                                                                                                              >  2:93373/1‑149 (MQ=255)
               gCACCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGgcgc                                                                                                                            >  8:155680/1‑149 (MQ=255)
                cACCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCCCCGCGCCCGGCTCGgcgcgca                                                                                                                           <  2:39816/149‑2 (MQ=255)
                 aCCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTa                                                                                                                          <  5:360379/149‑1 (MQ=255)
                 aCCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCCCCGCGCCCGGCTCGgcgcgcag                                                                                                                          <  2:240795/149‑3 (MQ=255)
                   cATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTAtg                                                                                                                        <  5:90194/149‑1 (MQ=255)
                   cATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTAtg                                                                                                                        >  2:269490/1‑149 (MQ=255)
                          gCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAAcg                                                                                                                 >  2:25773/1‑149 (MQ=255)
                           ccAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAAcgg                                                                                                                >  2:234377/1‑149 (MQ=255)
                                  ccaccaccaGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCg                                                                                                          >  5:259824/1‑148 (MQ=255)
                                  ccaccaccaGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCg                                                                                                          >  6:29138/1‑148 (MQ=255)
                                  ccaccaccaGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCg                                                                                                          >  3:46572/1‑148 (MQ=255)
                                       accaGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCcg                                                                                                    >  2:165232/1‑149 (MQ=255)
                                        ccaGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCAT‑CCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAAcg                                                                                                                 <  1:102581/134‑1 (MQ=255)
                                        ccaGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCAT‑CCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAAcg                                                                                                                 >  2:102581/1‑134 (MQ=255)
                                          cgcagGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCcggcg                                                                                                 <  1:167852/148‑1 (MQ=255)
                                               gTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTa                                                                                            >  3:125185/1‑149 (MQ=255)
                                               gTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTa                                                                                            >  5:55999/1‑149 (MQ=255)
                                               gTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTa                                                                                            >  5:212096/1‑149 (MQ=255)
                                                      tCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTg                                                                                     >  7:252347/1‑149 (MQ=255)
                                                       cAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGc                                                                                    >  3:152223/1‑149 (MQ=255)
                                                        aaCATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAg                                                                                        >  3:65121/1‑144 (MQ=255)
                                                        aaCATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAg                                                                                        <  4:65121/144‑1 (MQ=255)
                                                        aaCATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGc                                                                                    <  7:176787/148‑1 (MQ=255)
                                                        aaCATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCt                                                                                   <  4:369265/149‑1 (MQ=255)
                                                                cGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGTTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGgcgc                                                                            <  1:93373/148‑1 (MQ=255)
                                                                    ggCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGc                                                                       <  1:25773/149‑1 (MQ=255)
                                                                        gCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAg                                                                   <  6:212096/149‑1 (MQ=255)
                                                                         cACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGc                                                                  <  5:29138/149‑1 (MQ=255)
                                                                               gcgcGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGc                                                            <  1:165232/149‑1 (MQ=255)
                                                                                 gcgGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTAt                                                                                           >  5:138022/1‑116 (MQ=255)
                                                                                 gcgGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTAt                                                                                           <  6:138022/116‑1 (MQ=255)
                                                                                    gCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAg                                                                                        >  5:253644/1‑116 (MQ=255)
                                                                                    gCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAg                                                                                        <  6:253644/116‑1 (MQ=255)
                                                                                    gCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCg                                                       <  4:268182/149‑1 (MQ=255)
                                                                                     cATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCcggcg                                                                                                 <  5:50680/106‑1 (MQ=255)
                                                                                     cATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCcggcg                                                                                                 >  6:50680/1‑106 (MQ=255)
                                                                                     cATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGa                                                      <  1:269490/149‑1 (MQ=255)
                                                                                       tCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATg                                                    <  2:389772/149‑1 (MQ=255)
                                                                                         ccAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGc                                                   <  4:125185/148‑1 (MQ=255)
                                                                                          cAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCt                                                 >  5:203964/1‑149 (MQ=255)
                                                                                          cAAGCCACACCATCAGCCACCCCAAGGTGCTCTAGGCCCATGCCAACCTGCTCCCCGAGCCGCGCGCGGGGCGCTATCTGCAACGCCCGGCGTACCCGCCTGTCTATGACGTGCTGCCTCGCCCCCCCGATCAACAGCGGCCGATGcc                                                  >  8:368725/1‑148 (MQ=255)
                                                                                                         gCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGt                                  >  8:314291/1‑149 (MQ=255)
                                                                                                          ccAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGt                                  >  2:27469/1‑148 (MQ=255)
                                                                                                          ccAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTc                                 <  6:259824/149‑1 (MQ=255)
                                                                                                          ccAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTc                                 <  5:270257/149‑1 (MQ=255)
                                                                                                               gCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTgg                            <  6:55999/149‑1 (MQ=255)
                                                                                                               gCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTgg                            <  1:234377/149‑1 (MQ=255)
                                                                                                                cAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTggg                           >  8:234384/1‑149 (MQ=255)
                                                                                                                   ggTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGgct                        >  8:280423/1‑149 (MQ=255)
                                                                                                                    gTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGGCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGgctg                       <  4:360239/149‑1 (MQ=255)
                                                                                                                       ctctGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGgctgct                     <  6:185271/148‑1 (MQ=255)
                                                                                                                             gCCGATGGCGACCTGCGCGGCGAGCGGGGCGCGGGGCGCTATGTGGAACGGCCTGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAg               <  7:368725/148‑1 (MQ=255)
                                                                                                                              ccGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGcc             >  7:361100/1‑149 (MQ=255)
                                                                                                                                 aTGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCAt          >  4:189544/1‑149 (MQ=255)
                                                                                                                                  tGGCGACCTGCGCGCCGAGCCGGACGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGTCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATc         >  4:270724/1‑149 (MQ=255)
                                                                                                                                      gACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGa     <  7:155680/149‑1 (MQ=255)
                                                                                                                                      gACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCACAGCAGccccaatgac     <  3:353137/149‑8 (MQ=255)
                                                                                                                                         cTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGcc             <  2:219305/138‑1 (MQ=255)
                                                                                                                                         cTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGcc             >  1:219305/1‑138 (MQ=255)
                                                                                                                                           gcgcccCGCGCCCGGCTCGGCGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGc  >  5:98031/1‑147 (MQ=255)
                                                                                                                                                   |                                                                                                                                          
ACCCGCAGGGCACCCGCACCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGC  >  NC_002947/4548878‑4549163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑