Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,549,029 G→C 6.1% P447P (CCG→CCC)  pydB → bifunctional D‑hydantoinase/dihydropyrimidinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,549,0290GC 6.1% 211.3 / 2.5 65P447P (CCG→CCC) pydBbifunctional D‑hydantoinase/dihydropyrimidinase
Reads supporting (aligned to +/- strand):  ref base G (31/30);  new base C (2/2);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

GGCACCCGCACCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCA  >  NC_002947/4548886‑4549176
                                                                                                                                               |                                                                                                                                                   
ggCACCCGCACCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGgcgc                                                                                                                                                >  6:360379/1‑149 (MQ=255)
ggCACCCGCACCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCCCCGCGCCCGgctc                                                                                                                                                >  1:39816/1‑147 (MQ=255)
 gCACCCGCACCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGcgcg                                                                                                                                               >  8:176787/1‑149 (MQ=255)
     ccGCACCATCTCCGCCAAAACCCACCACCAGCAGGAGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGgc                                                                                                                                           >  2:93373/1‑149 (MQ=255)
       gCACCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGgcgc                                                                                                                                         >  8:155680/1‑149 (MQ=255)
        cACCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCCCCGCGCCCGGCTCGgcgcgca                                                                                                                                        <  2:39816/149‑2 (MQ=255)
         aCCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTa                                                                                                                                       <  5:360379/149‑1 (MQ=255)
         aCCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCCCCGCGCCCGGCTCGgcgcgcag                                                                                                                                       <  2:240795/149‑3 (MQ=255)
           cATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTAtg                                                                                                                                     <  5:90194/149‑1 (MQ=255)
           cATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTAtg                                                                                                                                     >  2:269490/1‑149 (MQ=255)
                  gCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAAcg                                                                                                                              >  2:25773/1‑149 (MQ=255)
                   ccAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAAcgg                                                                                                                             >  2:234377/1‑149 (MQ=255)
                          ccaccaccaGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCg                                                                                                                       >  5:259824/1‑148 (MQ=255)
                          ccaccaccaGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCg                                                                                                                       >  3:46572/1‑148 (MQ=255)
                          ccaccaccaGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCg                                                                                                                       >  6:29138/1‑148 (MQ=255)
                               accaGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCcg                                                                                                                 >  2:165232/1‑149 (MQ=255)
                                ccaGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCAT‑CCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAAcg                                                                                                                              >  2:102581/1‑134 (MQ=255)
                                ccaGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCAT‑CCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAAcg                                                                                                                              <  1:102581/134‑1 (MQ=255)
                                  cgcagGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCcggcg                                                                                                              <  1:167852/148‑1 (MQ=255)
                                       gTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTa                                                                                                         >  5:55999/1‑149 (MQ=255)
                                       gTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTa                                                                                                         >  5:212096/1‑149 (MQ=255)
                                       gTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTa                                                                                                         >  3:125185/1‑149 (MQ=255)
                                              tCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTg                                                                                                  >  7:252347/1‑149 (MQ=255)
                                               cAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGc                                                                                                 >  3:152223/1‑149 (MQ=255)
                                                aaCATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAg                                                                                                     >  3:65121/1‑144 (MQ=255)
                                                aaCATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAg                                                                                                     <  4:65121/144‑1 (MQ=255)
                                                aaCATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGc                                                                                                 <  7:176787/148‑1 (MQ=255)
                                                aaCATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCt                                                                                                <  4:369265/149‑1 (MQ=255)
                                                        cGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGTTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGgcgc                                                                                         <  1:93373/148‑1 (MQ=255)
                                                            ggCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGc                                                                                    <  1:25773/149‑1 (MQ=255)
                                                                gCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAg                                                                                <  6:212096/149‑1 (MQ=255)
                                                                 cACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGc                                                                               <  5:29138/149‑1 (MQ=255)
                                                                       gcgcGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGc                                                                         <  1:165232/149‑1 (MQ=255)
                                                                         gcgGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTAt                                                                                                        <  6:138022/116‑1 (MQ=255)
                                                                         gcgGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTAt                                                                                                        >  5:138022/1‑116 (MQ=255)
                                                                            gCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAg                                                                                                     >  5:253644/1‑116 (MQ=255)
                                                                            gCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAg                                                                                                     <  6:253644/116‑1 (MQ=255)
                                                                            gCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCg                                                                    <  4:268182/149‑1 (MQ=255)
                                                                             cATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCcggcg                                                                                                              >  6:50680/1‑106 (MQ=255)
                                                                             cATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCcggcg                                                                                                              <  5:50680/106‑1 (MQ=255)
                                                                             cATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGa                                                                   <  1:269490/149‑1 (MQ=255)
                                                                               tCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATg                                                                 <  2:389772/149‑1 (MQ=255)
                                                                                 ccAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGc                                                                <  4:125185/148‑1 (MQ=255)
                                                                                  cAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCt                                                              >  5:203964/1‑149 (MQ=255)
                                                                                  cAAGCCACACCATCAGCCACCCCAAGGTGCTCTAGGCCCATGCCAACCTGCTCCCCGAGCCGCGCGCGGGGCGCTATCTGCAACGCCCGGCGTACCCGCCTGTCTATGACGTGCTGCCTCGCCCCCCCGATCAACAGCGGCCGATGcc                                                               >  8:368725/1‑148 (MQ=255)
                                                                                                 gCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGt                                               >  8:314291/1‑149 (MQ=255)
                                                                                                  ccAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGt                                               >  2:27469/1‑148 (MQ=255)
                                                                                                  ccAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTc                                              <  5:270257/149‑1 (MQ=255)
                                                                                                  ccAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTc                                              <  6:259824/149‑1 (MQ=255)
                                                                                                       gCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTgg                                         <  6:55999/149‑1 (MQ=255)
                                                                                                       gCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTgg                                         <  1:234377/149‑1 (MQ=255)
                                                                                                        cAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTggg                                        >  8:234384/1‑149 (MQ=255)
                                                                                                           ggTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGgct                                     >  8:280423/1‑149 (MQ=255)
                                                                                                            gTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGGCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGgctg                                    <  4:360239/149‑1 (MQ=255)
                                                                                                               ctctGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGgctgct                                  <  6:185271/148‑1 (MQ=255)
                                                                                                                     gCCGATGGCGACCTGCGCGGCGAGCGGGGCGCGGGGCGCTATGTGGAACGGCCTGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAg                            <  7:368725/148‑1 (MQ=255)
                                                                                                                      ccGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGcc                          >  7:361100/1‑149 (MQ=255)
                                                                                                                         aTGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCAt                       >  4:189544/1‑149 (MQ=255)
                                                                                                                          tGGCGACCTGCGCGCCGAGCCGGACGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGTCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATc                      >  4:270724/1‑149 (MQ=255)
                                                                                                                              gACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGa                  <  7:155680/149‑1 (MQ=255)
                                                                                                                              gACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCACAGCAGccccaatgac                  <  3:353137/149‑8 (MQ=255)
                                                                                                                                 cTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGcc                          <  2:219305/138‑1 (MQ=255)
                                                                                                                                 cTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGcc                          >  1:219305/1‑138 (MQ=255)
                                                                                                                                   gcgcccCGCGCCCGGCTCGGCGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGc               >  5:98031/1‑147 (MQ=255)
                                                                                                                                            gCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTcc    <  6:203964/149‑1 (MQ=255)
                                                                                                                                               gggCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCca  >  6:158014/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GGCACCCGCACCATCTCCGCCAAAACCCACCACCAGCAGGTGGACTTCAACATTTTCGAAGGCCGCACCGTGCGCGGCATCCCAAGCCACACCATCAGCCAGGGCAAGGTGCTCTGGGCCGATGGCGACCTGCGCGCCGAGCCGGGCGCGGGGCGCTATGTGGAACGGCCGGCGTACCCGGCGGTGTATGAGGTGCTGGGGCGCCGGGCCGAGCATCAGCGGCCGATGCCTGTTCCGCGCTGAAGTCATTGGGGCTGCTGTGCAGCCCATCGCGACGCAAGGCCGCTCCCA  >  NC_002947/4548886‑4549176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑