Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,807,254 G→A 6.9% S213F (TCC→TTC)  PP_4234 ← bifunctional aldehyde oxidase/xanthine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,807,2540GA 6.9% 169.8 / 2.1 58S213F (TCC→TTC) PP_4234bifunctional aldehyde oxidase/xanthine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (30/24);  new base A (2/2);  total (32/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

CTCGGGCGGGTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTGCGCTTGGCCTGGCGGAAGCG  >  NC_002947/4807109‑4807402
                                                                                                                                                 |                                                                                                                                                    
cTCGGGCGGGTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGgag                                                                                                                                                    >  2:32663/1‑148 (MQ=255)
   gggcgggTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGaa                                                                                                                                                  <  3:310340/147‑1 (MQ=255)
           tGTCCAGCTGCTCGTCAATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCAtcgtcg                                                                                                                                        <  4:246309/149‑1 (MQ=255)
             tCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTCCAGCCTGAAGAACTcca                                                                                                                                             >  1:137067/1‑140 (MQ=255)
             tCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTCCAGCCTGAAGAACTcca                                                                                                                                             <  2:137067/142‑3 (MQ=255)
               cAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCAtcgtcgtcg                                                                                                                                     >  4:290675/1‑148 (MQ=255)
                 gctgcgCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAAc                                                                                                                                  >  2:196061/1‑149 (MQ=255)
                     ctCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTGCAGGCTGGAGAACTCATCGTCGTCGAACGGCg                                                                                                                              >  3:38965/1‑149 (MQ=255)
                             tGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGcca                                                                                                                      >  4:368983/1‑149 (MQ=255)
                                       gTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCa                                                                                                            >  8:36690/1‑149 (MQ=255)
                                                      cTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCTGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCt                                                                                               <  5:60772/147‑1 (MQ=255)
                                                         ggTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCa                                                                                           <  3:290675/148‑1 (MQ=255)
                                                                tCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTc                                                                                   <  1:32663/149‑1 (MQ=255)
                                                                    cGTTCTGGGTGGGCTTCTTCGACCACACAGGCTTGCGGTTGAGAATTGTGTGGAACAGCATTGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACTTCGTGGCGCCACCAACCCGCACGAAGCGGAAGCTGCCATAAGGGTctc                                                                                 <  2:266629/147‑3 (MQ=255)
                                                                       gCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACGCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTg                                                                             <  8:39068/148‑1 (MQ=255)
                                                                           gTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCAt                                                                                          <  5:350762/131‑1 (MQ=255)
                                                                           gTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCAt                                                                                          >  6:350762/1‑131 (MQ=255)
                                                                           gTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTa                                                                          >  6:359329/1‑147 (MQ=255)
                                                                                gctgctTCGACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAgcggc                                                                   >  6:171464/1‑149 (MQ=255)
                                                                                   gctTCGACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTgg                                                                            >  7:16442/1‑137 (MQ=255)
                                                                                   gctTCGACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTgg                                                                            <  8:16442/137‑1 (MQ=255)
                                                                                   gctTCGACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGcc                                                                >  3:357664/1‑149 (MQ=255)
                                                                                        gACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTggg                                                            >  7:278011/1‑148 (MQ=255)
                                                                                              acCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAgc                                                                      >  7:165853/1‑132 (MQ=255)
                                                                                              acCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAgc                                                                      <  8:165853/132‑1 (MQ=255)
                                                                                              acCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCACACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGt                                                      >  2:87675/1‑148 (MQ=255)
                                                                                                cGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCAAGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCTTAAc                                                   >  2:396151/1‑149 (MQ=255)
                                                                                                  gCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACgg                                                 <  7:36690/149‑1 (MQ=255)
                                                                                                    ttGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGTCTGGAGAACTCATCGTCGTCGAACGGCTTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCTTAAGGGTCGCGCTg                                                                             <  5:340134/119‑1 (MQ=255)
                                                                                                    ttGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGAt                                               >  5:372484/1‑149 (MQ=255)
                                                                                                      gCGGTTGAGTTTGGTGGGGAACAGCATGGAGTTCTTCAGGCTTGAGAAATCATCGTCGTCGTACGTCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTTACATAAGGGTCTCTGTGGTACAGAGGCGCCTGGGCTCCGTATCGGAtg                                              <  6:365414/148‑2 (MQ=255)
                                                                                                         gTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTg                                                              >  2:31541/1‑129 (MQ=255)
                                                                                                         gTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTg                                                              <  1:31541/129‑1 (MQ=255)
                                                                                                             agaATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCctgct                                       <  3:368983/148‑1 (MQ=255)
                                                                                                             agaATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCctgct                                       <  1:196061/148‑1 (MQ=255)
                                                                                                              gaATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGa                                     >  4:20298/1‑149 (MQ=255)
                                                                                                              gaATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGa                                     <  5:171464/149‑1 (MQ=255)
                                                                                                              gaATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGa                                     >  2:29006/1‑149 (MQ=255)
                                                                                                               aaTGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGAt                                    >  6:87348/1‑149 (MQ=255)
                                                                                                                aTGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGAt                                    <  6:372484/148‑1 (MQ=255)
                                                                                                                 tggtggGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATc                                              >  1:385642/1‑137 (MQ=255)
                                                                                                                 tggtggGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATc                                              <  2:385642/137‑1 (MQ=255)
                                                                                                                     ggggAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACt                              >  3:4218/1‑149 (MQ=255)
                                                                                                                        gAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTg                             >  8:229729/1‑147 (MQ=255)
                                                                                                                             gCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTAATTGAACTGCAGCTTg                      >  7:300897/1‑149 (MQ=255)
                                                                                                                              cATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGGAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTgc                     >  8:355167/1‑149 (MQ=255)
                                                                                                                              cATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTgc                     >  1:108067/1‑149 (MQ=255)
                                                                                                                              cATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTgc                     <  1:29006/149‑1 (MQ=255)
                                                                                                                              cATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTgc                     >  7:321141/1‑149 (MQ=255)
                                                                                                                               gatgAGTTCTCCAGCCTGAAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCGTGCTGATTGAACTGCAGCTTgcg                    >  4:40360/4‑149 (MQ=255)
                                                                                                                                 ggAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTgcgc                   >  8:79408/1‑148 (MQ=255)
                                                                                                                                 ggAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTgcgc                   <  7:79408/148‑1 (MQ=255)
                                                                                                                                  gAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTGCGCt                  <  2:108067/148‑1 (MQ=255)
                                                                                                                                   aGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTGCGCTTg                >  2:145500/1‑149 (MQ=255)
                                                                                                                                        ttcAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATc                                              <  3:194143/114‑1 (MQ=255)
                                                                                                                                        ttcAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATc                                              >  4:194143/1‑114 (MQ=255)
                                                                                                                                          cAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTGCGCTTGGCCTGGc         >  3:353459/1‑149 (MQ=255)
                                                                                                                                           aGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTGCGCTTGGCCTGGc         <  3:20298/148‑1 (MQ=255)
                                                                                                                                              cTGAAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTGCGCTTGGCCTGGCGGaa     <  3:40360/149‑1 (MQ=255)
                                                                                                                                                 gagaACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTGCGCTTGGCCTGGCGGAAgcg  >  3:297904/1‑149 (MQ=255)
                                                                                                                                                 gagaACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTGCGCTTGGCCTGGCGGAAgcg  >  3:82932/1‑149 (MQ=255)
                                                                                                                                                 gagaACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGAACGAAGCGGAAGCTGCCATAAGGTTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTGCGCTTGGCCTGGCGGAAgcg  >  6:414180/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CTCGGGCGGGTTGTCCAGCTGCTCGGCCATGCGCTTGGCGTAGAACAACCCCTGCTCGGTCAGGTCGCCGTGCTGGTTGGGCTGCTTCGACCACACCGGCTTGCGGTTGAGAATGGTGGGGAACAGCATGGAGTTCTTCAGGCTGGAGAACTCATCGTCGTCGAACGGCGTGGCGCCACCCACCCGCACGAAGCGGAAGCTGCCATAAGGGTCGCGCTGGTACAGCGGCGCCTGGGCACCGTAACGGATCGCCTGCTGATTGAACTGCAGCTTGCGCTTGGCCTGGCGGAAGCG  >  NC_002947/4807109‑4807402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑