| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,852,459 | A→T | 17.4% | intergenic (‑130/+167) | recR ← / ← ybaB | recombination protein RecR/nucleoid‑associated broad specificity regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,852,459 | 0 | A | T | 17.4% | 47.1 / 4.8 | 23 | intergenic (‑130/+167) | recR/ybaB | recombination protein RecR/nucleoid‑associated broad specificity regulator |
| Reads supporting (aligned to +/- strand): ref base A (12/7); new base T (2/2); total (14/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.17e-01 | |||||||||||
GCTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAAGCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGTTGTCAGCGGCG > NC_002947/4852324‑4852604 | gCTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAAGCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGAagc > 8:72156/1‑139 (MQ=255)gCTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAAGCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGAagc < 7:72156/139‑1 (MQ=255) cTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAAGCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCTTCGCAGccctttcgc < 8:213041/149‑7 (MQ=39) caCAATTCAATAAGCGCCGGTTAAACCCCCGGGGGGGCGGTGGCGTCAAATCCCCTCCCCAAAAGCCTGCAAGGTCTGGTTTTTTGTGGCCGCGGCCTTGTGTCGCTAATGGGCTGCTAAGCAGCCCCGGCACTCTGTGCTGCGAAGCt < 8:317965/148‑1 (MQ=255) aaTAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAAGCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCTTCGCAGccctttc < 8:103630/123‑5 (MQ=38) aaTAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAAGCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCTTCGCAGccctttc > 7:103630/1‑119 (MQ=38) agagGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAAGCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCgggg > 3:362675/1‑149 (MQ=255) cgttttATACCTCGGGGGGGGGGTGGGTTAAAATCACCTCCCCAAACGCCTTCATGGTTTGTTTTTTTGTGGGAGCGGCTTTGTGGCGCTAATGGGCTGCTATGCTGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCt < 4:362675/146‑1 (MQ=255) gggggggcgggggcCTAAAACCCCCCCCCCCAACGCCGGCCCGGTCTGCTTTTTTGGGGCCGCGCCCTGGGGGCGCCAACGGGCGGCGACGCCCCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTc < 3:68671/147‑1 (MQ=255) ggTCTGCTTTTTTGTGGAAGCGGCCTTGTGTCGCGAAAGGGCTGCGAagca > 5:366316/1‑51 (MQ=255) ggTCTGCTTTTTTGTGGAAGCGGCCTTGTGTCGCGAAAGGGCTGCGAagca < 6:366316/51‑1 (MQ=255) ccTTGTGTCGCGAAAGGGCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAAc > 5:197440/1‑149 (MQ=255) gcgAAAGGGCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCCCtgt > 4:276538/1‑149 (MQ=255) gcgAAAGGGCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACtgt > 1:177636/1‑149 (MQ=255) gccggggCTGCTTCGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACtgttg > 8:97855/5‑149 (MQ=255) gAAAGGGCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACtgttg > 5:366425/1‑149 (MQ=255) aaGGGCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACtgttgt > 4:340973/1‑148 (MQ=255) gCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGTCACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGTTGTCAgcg > 1:128621/1‑149 (MQ=255) tGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGa < 7:273612/97‑1 (MQ=255) tGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGa > 8:273612/1‑97 (MQ=255) tGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACt < 6:294172/137‑1 (MQ=255) tGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACt > 5:294172/1‑137 (MQ=255) gCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGTTGTCAgcggcg > 6:7384/1‑149 (MQ=255) | GCTCATGGGAAAGGCCTGACAATTCAATAAGAGGCGGTTTATACCCAGCGGGGGCGGTGGCGTCAAATCACCTCGCCCAAAGCCTGCAAGGTCTGCTTTTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCTGCGAAGCAGCCCCGGCACTCTGTGCTGCGAAGCTGAAATCCCGGGGCCGCTTCGCGCCCCTTTCGCGACACAAGGCCGCTCCCACAAAAAAGCAGACCGCAACTGGGTTGAAATCTCCGCAAAAAAAACCGCCACTGTTGTCAGCGGCG > NC_002947/4852324‑4852604 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |