Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,899,879 G→T 5.6% C93F (TGC→TTC)  PP_4308 → AsnC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,899,8790GT 5.6% 232.8 / 3.4 71C93F (TGC→TTC) PP_4308AsnC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (31/36);  new base T (2/2);  total (33/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

AGCGTGGCGTGCTGCGCGGCTATACCGTGGAGGTCGACCCGCGCGCCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCG  >  NC_002947/4899732‑4900027
                                                                                                                                                   |                                                                                                                                                    
agCGTGGCGTGCTGCGCCGCTATACCGTGGAGGTCGACCCGCGCGCCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGc                                                                                                                                                     <  5:345063/149‑1 (MQ=255)
   gttgcgtgCTGCGCGGCTATACCGTGGAGGTCGACCCGCGCGCCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGAc                                                                                                                                                  <  3:36991/146‑1 (MQ=255)
    tggcgtgCTGCGCGGCTATACCGTGGAGGTCGACCCGCGCGCCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGAc                                                                                                                                                  >  4:23411/1‑148 (MQ=255)
    tggcgtgCTGCGCGGCTATACCGTGGAGGTCGACCCGCGCGCCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGAc                                                                                                                                                  >  7:369585/1‑148 (MQ=255)
           ctgcGCGGCTATACCGTGGAGGTCGACCCGCGCGCCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGTGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCAt                                                                                                                                          <  7:131650/149‑1 (MQ=255)
           ctgcGCGGCTATACCGTGGAGGTCGACCCGCGCGCCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCa                                                                                                                                           <  6:75097/148‑1 (MQ=255)
            tgcGCGGCTATACCGTGGAGGTCGACCCGCGCGCCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCAtt                                                                                                                                         >  8:182286/1‑149 (MQ=255)
                cgGCTATACCGTGGAGGTCCACCCGCGCGCCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCg                                                                                                                                     <  8:92542/149‑1 (MQ=255)
                      taCCGTGGAGGTCGACCCGCGCGCCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGa                                                                                                                                 <  2:170798/147‑1 (MQ=255)
                      taCCGTGGAGGTCGACCCGCGCGCCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGAc                                                                                                                                <  7:182286/148‑1 (MQ=255)
                        ccGTGGAGGTCGACCCGCGCGCCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGc                                                                                                                             <  1:247163/149‑1 (MQ=255)
                         cGTGGAGGTCGACCCGCGCGCCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCt                                                                                                                            >  1:7377/1‑149 (MQ=255)
                          gTGGATGTCGACCCGCTCGCCTTTTGCTAACAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCATGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCtt                                                                                                                           <  3:17933/149‑1 (MQ=255)
                              aGGTCGACCCGCGCGCCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTtcgtc                                                                                                                       >  3:90758/1‑149 (MQ=255)
                                gTCGACCCGCGCGCCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGc                                                                                                                     >  8:274255/1‑149 (MQ=255)
                                   gACCCGCGCGCCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATTCGACAAGGTCATTGGCGAAGACTGCTTCGTCGccc                                                                                                                   >  4:356623/1‑148 (MQ=255)
                                   gACCCGCGCGCCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATTCGACAAGGTCATTGGCGAAGACTGCTTCGTCGc                                                                                                                     >  4:362459/1‑146 (MQ=255)
                                   gACCAGCGCGCCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGccc                                                                                                                   >  4:301872/1‑148 (MQ=255)
                                     cccGCGCGCCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGAc                                                                                                                >  6:310202/1‑149 (MQ=255)
                                      ccGCGCGCCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACt                                                                                                               >  6:155637/1‑149 (MQ=255)
                                      ccGCGCGCCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACt                                                                                                               <  3:324602/149‑1 (MQ=255)
                                        gcgcgcCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTg                                                                                                              >  8:21137/1‑148 (MQ=255)
                                          gcgcCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCAt                                                                                                           >  4:358657/1‑149 (MQ=255)
                                           cgcCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCAtg                                                                                                          <  2:7377/149‑1 (MQ=255)
                                                  gccTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCt                                                                                                    <  8:336816/146‑1 (MQ=255)
                                                   gCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTc                                                                                                   <  5:36523/148‑1 (MQ=255)
                                                    cTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCg                                                                                                                                                    >  4:110091/1‑98 (MQ=255)
                                                    cTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCg                                                                                                                                                    <  3:110091/98‑1 (MQ=255)
                                                     tACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGAt                                                                                                >  8:155195/1‑149 (MQ=255)
                                                      aCCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACt                                                                                                                               >  8:244324/1‑117 (MQ=255)
                                                      aCCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACt                                                                                                                               <  7:244324/117‑1 (MQ=255)
                                                       ccAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATg                                                                                               <  7:21137/148‑1 (MQ=255)
                                                               aGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTg                                                                                      >  3:144084/1‑149 (MQ=255)
                                                                 gCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGccc                                                                                                                   <  5:199070/118‑1 (MQ=255)
                                                                 gCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGccc                                                                                                                   >  6:199070/1‑118 (MQ=255)
                                                                 gCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGa                                                                                    <  8:127839/149‑1 (MQ=255)
                                                                 gCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGa                                                                                    <  8:369585/149‑1 (MQ=255)
                                                                    aTCGTGTGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGacac                                                                                 >  3:202588/1‑149 (MQ=255)
                                                                    aTCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGccac                                                                                 <  4:50327/149‑1 (MQ=255)
                                                                          cgcATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGc                                                                            <  7:274255/148‑1 (MQ=255)
                                                                           gcATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTc                                                                          <  3:23411/149‑1 (MQ=255)
                                                                           gcATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTc                                                                          <  5:310202/149‑1 (MQ=255)
                                                                             aTCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGacac                                                                                 <  2:179518/140‑1 (MQ=255)
                                                                             aTCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGacac                                                                                 >  1:179518/1‑140 (MQ=255)
                                                                                cgtccgCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCt                                                                     >  4:338005/1‑149 (MQ=255)
                                                                                 gtccgCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATgg                                                                                              <  3:158073/123‑1 (MQ=255)
                                                                                 gtccgCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATgg                                                                                              >  4:158073/1‑123 (MQ=255)
                                                                                  tccgCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTg                                                                                      >  2:322375/1‑130 (MQ=255)
                                                                                  tccgCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTg                                                                                      <  1:322375/130‑1 (MQ=255)
                                                                                    cgCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCAt                                                                  <  8:222771/148‑1 (MQ=255)
                                                                                         ccGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAAtg                                                             <  5:151407/148‑1 (MQ=255)
                                                                                              gAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTgg                                                       <  4:202588/149‑1 (MQ=255)
                                                                                               aaGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGGTGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGc                                                      >  5:360969/1‑149 (MQ=255)
                                                                                                  cTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCAt                                                                  <  2:344293/134‑1 (MQ=255)
                                                                                                    gCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCgaga                                                 >  1:152836/1‑149 (MQ=255)
                                                                                                    gCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCgaga                                                 >  5:141545/1‑149 (MQ=255)
                                                                                                     cAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATTCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCgaga                                                 <  3:356623/148‑1 (MQ=255)
                                                                                                     cAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATTCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCgaga                                                 <  3:362459/148‑1 (MQ=255)
                                                                                                       ggaggTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCa                                              <  3:301872/149‑1 (MQ=255)
                                                                                                                          aTTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCaagaa                           <  7:155195/149‑1 (MQ=255)
                                                                                                                             gCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGAc                        >  3:13167/1‑149 (MQ=255)
                                                                                                                              ccATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCAtgt                                                                                                         <  4:213774/67‑1 (MQ=255)
                                                                                                                              ccATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGAcc                       >  5:265963/1‑149 (MQ=255)
                                                                                                                              ccATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGAcc                       >  5:379756/1‑149 (MQ=255)
                                                                                                                                 tCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCg                    <  3:358657/149‑1 (MQ=255)
                                                                                                                                 tCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCg                    >  2:6735/1‑149 (MQ=255)
                                                                                                                                  cccTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCgg                   >  3:164847/1‑149 (MQ=255)
                                                                                                                                    cTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGt                  >  4:119719/1‑148 (MQ=255)
                                                                                                                                        gTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAgc             <  3:119719/149‑1 (MQ=255)
                                                                                                                                            aCCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAgcggc          <  3:338005/148‑1 (MQ=255)
                                                                                                                                                aaTGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTg     >  4:110961/1‑149 (MQ=255)
                                                                                                                                                   gCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTgccg  >  4:174133/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AGCGTGGCGTGCTGCGCGGCTATACCGTGGAGGTCGACCCGCGCGCCTTTGGCTACCAGTTGCAGGCGATCGTGCGCATCCGTCCGCTACCGGGGAAGCTGCAGGAGGTGGAGCGCCAGATCATTGCCATCCCTGAGTTCACCGAATGCGACAAGGTCATTGGCGAAGACTGCTTCGTCGCCCGACTGCATGTGCGCTCGATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCG  >  NC_002947/4899732‑4900027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑