| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,970,603 | T→C | 5.5% | intergenic (‑361/+33) | fliC ← / ← PP_4379 | flagellin/3‑oxoacyl‑ACP synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,970,603 | 0 | T | C | 5.5% | 214.8 / 2.4 | 73 | intergenic (‑361/+33) | fliC/PP_4379 | flagellin/3‑oxoacyl‑ACP synthase |
| Reads supporting (aligned to +/- strand): ref base T (31/38); new base C (2/2); total (33/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.32e-01 | |||||||||||
ATCCCGTCGGTGGAGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAA > NC_002947/4970457‑4970745 | aTCCCGGCGGTGGAGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTg < 1:355668/148‑1 (MQ=255) tCCCGTCGGTGGAGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCTGATGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTgg > 1:116150/1‑149 (MQ=255) gTCGGTGGAGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGgccgcc < 3:252163/149‑1 (MQ=255) cGGTGGAGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCgg < 3:308777/149‑1 (MQ=255) ggTGGAGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCggg < 2:296983/149‑1 (MQ=255) ggAGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCGTACATTGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGgcgc < 6:322479/149‑1 (MQ=255) gacgggatGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCt < 1:305199/142‑1 (MQ=255) agggggTGAGGCTGCTAAGAAGGATACGCGATGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCtt < 4:264940/146‑1 (MQ=255) gggTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCCGGCGGCCACCGCCCGGCGCttttt < 1:363824/149‑2 (MQ=38) gTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAgg > 1:290821/1‑149 (MQ=255) gTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCCGGCGGCCACCGCCCGGCGCttttttt < 6:80209/149‑4 (MQ=38) ggCTGTGAAGAAGGAGACGCGATGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCt > 4:392318/1‑149 (MQ=255) tGCGAAGAAGGAGACGCGATGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGAc > 6:66743/1‑149 (MQ=255) ggAGACGCGATGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAg > 5:74987/1‑149 (MQ=255) ggAGACGCGATGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAg > 4:354501/1‑149 (MQ=255) gccgcgATGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTTAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAggg < 3:21127/129‑1 (MQ=255) cgcgATGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTcgcg < 5:285319/149‑1 (MQ=255) cgcgATGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTcgcg < 6:74987/149‑1 (MQ=255) cgcgAGGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTcgcg < 3:226450/149‑1 (MQ=255) gcgATGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCgg > 4:348196/1‑149 (MQ=255) gATGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTc < 5:398793/143‑1 (MQ=255) gATGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTc > 6:398793/1‑143 (MQ=255) ggTGGCACCGGCT‑TTGCCGGTGGTCGCGGGTGAACCCGCGCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAgg < 2:196891/149‑1 (MQ=255) gcgaacACCGGCA‑AAGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAg > 1:206506/6‑148 (MQ=255) gTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTcgcg < 3:181776/141‑1 (MQ=255) gTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTcgcg > 4:181776/1‑141 (MQ=255) tGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGAt < 5:9677/149‑1 (MQ=255) ggCACCGGCT‑TTGCCGGTGTTCACGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATg < 4:271957/149‑1 (MQ=255) cGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGcc > 5:144710/1‑147 (MQ=255) gCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGc < 8:230863/149‑1 (MQ=255) ccGGTGTACGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCAc < 7:17978/148‑1 (MQ=255) cGGTGTTCGCGGGTGCACCCGCTCCTACAATGGGCCGCGTTAGGCAGAAGCTTATTTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCggg < 1:71664/95‑1 (MQ=255) cGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCggg > 2:71664/1‑94 (MQ=255) cGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGa < 6:144710/149‑1 (MQ=255) cGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGa > 1:363828/1‑149 (MQ=255) ggTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGAc < 7:264865/149‑1 (MQ=255) attCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAgg > 5:327752/2‑149 (MQ=255) cgcgGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGATGACTGGGATCAGTCGCGGTCCAGGATGGCCGAGCCCCACGCCAGGccc > 4:244087/1‑149 (MQ=255) gcgGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCa > 5:96647/1‑149 (MQ=255) gTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGcc < 6:96647/149‑1 (MQ=255) aCCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAg < 8:111847/104‑1 (MQ=255) aCCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAg > 7:111847/1‑104 (MQ=255) tCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACt > 2:173687/1‑148 (MQ=255) cTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGTGCGGTGGCCGACGGGCGCTTTAAGGGAATTAATGGGATCATTCGCGTTACAGGATGGCCGAGCCCAACCACAGGCCAACGCCAAAGCCaataat > 4:13216/1‑144 (MQ=255) cTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGAt > 5:149636/1‑149 (MQ=255) aCAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGAAGAAAAAAAAGAGCAGGGCGGTGGCCGCCGGGCGCTTATAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCg > 8:247603/1‑149 (MQ=255) cAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGc < 3:152002/149‑1 (MQ=255) cAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGc < 2:363828/149‑1 (MQ=255) aaTGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCCATGATCGcc > 3:252515/1‑149 (MQ=255) tGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCAc > 5:111903/1‑149 (MQ=255) ggCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCAcgc < 2:290821/149‑1 (MQ=255) gCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCAcgcg > 2:408042/1‑149 (MQ=255) gCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCCCGCGCtt > 7:277614/1‑149 (MQ=255) cGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCt < 6:327752/147‑1 (MQ=255) ggCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGc < 3:392318/148‑1 (MQ=255) gCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTg < 8:54989/149‑1 (MQ=255) cAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGACGAGCCCCACGACAGGCCCACGCCAAAGCCAGTGATCGCCACGCGCTTCCAGCTGc > 8:188202/1‑149 (MQ=255) aGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGc > 3:127622/1‑148 (MQ=255) aaGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCAt > 6:52260/1‑149 (MQ=255) aGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATc < 8:154286/149‑1 (MQ=255) cTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGa < 6:149636/149‑1 (MQ=255) cTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGa < 1:173687/149‑1 (MQ=255) cTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGa < 6:111903/149‑1 (MQ=255) ttttCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCAt > 3:41715/1‑149 (MQ=255) gcattaaaaaaaGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCtt < 3:13216/144‑1 (MQ=255) gCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCa < 2:251266/142‑1 (MQ=255) gCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCa > 1:251266/1‑142 (MQ=255) aGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCATCTGCCATCGAGCATGTGCTTCt > 8:329271/1‑149 (MQ=255) aGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCt < 2:206506/149‑1 (MQ=255) ctaaaaGCGCCCGGCGGCCACCGCCCGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTgcag > 1:31586/3‑149 (MQ=39) aaGCGCCCGGCGGCCACCGCCCGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTgcagcag > 5:87723/1‑148 (MQ=255) cgcCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTGCagcagcagc < 7:370013/149‑1 (MQ=255) gggCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGAGCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGaa < 2:116150/149‑1 (MQ=255) | ATCCCGTCGGTGGAGCGGGTGAGGCTGCGAAGAAGGAGACGCGATGGGTGGCACCGGCT‑TTGCCGGTGTTCGCGGGTGAACCCGCTCCTACAATGGGCTGCGTTAGGCAGAAGCTTA‑TTTTCGGCAGAAAAAAAAGCGCCGGGCGGTGGCCGCCGGGCGCTTTTAGGGGCTGACTGGGATCAGTCGCGGTACAGGATGGCCGAGCCCCACGACAGGCCCACGCCAAAGCCACTGATCGCCACGCGCTTCCAGCTGCCATCGAGCATGTGCTTCTGCAGCAGCAGCGGAA > NC_002947/4970457‑4970745 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |