Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,989,290 C→T 10.7% G157S (GGC→AGC) ‡ PP_4398 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,989,2900CT10.7% 173.1 / 8.9 65G157S (GGC→AGC) ‡PP_4398MFS transporter
Reads supporting (aligned to +/- strand):  ref base C (26/32);  new base T (4/3);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01

GTACTGAGCGACTGCGGCACGCGCTTGATGAAGAACTTCGGCTCCATCGGTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCTGCTCATTGAGCCAGCTCTCGATGACCATGTATTG  >  NC_002947/4989142‑4989428
                                                                                                                                                    |                                                                                                                                          
gTACTGAGCGACTGCGGCACGCGCTTGATGAAGAACTTCGGCTCCATCGGTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGcc                                                                                                                                            >  7:14384/1‑149 (MQ=255)
 tACTGAGCGACTGCGGCACGCGCTTGATGAAGAACTTCGGCTCCATCGGTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACAAACATCAGCAGCTCAGGGcc                                                                                                                                            >  2:290186/1‑148 (MQ=255)
   cTGAGCGACTGCGGCACGCGCTTGATGAAGAACTTCGGCTCCATCGGTGCCGGGCGCAAGGGCGCCGGGGGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGa                                                                                                                                          <  6:234515/148‑1 (MQ=255)
      aGCGACTGCGGCACGCGCTTGATGAAGAACTTCGGCTCCATCGGTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTg                                                                                                                                      >  7:155378/1‑149 (MQ=255)
        cGACTGCGGCACGCGCTTGATGAAGAACTTCGGCTCCATCGGTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCg                                                                                                                                    >  7:238992/1‑149 (MQ=255)
         gACTGCGGCACGCGCTTGATGAAGAACTTCGGCTCCATCGGTGCCGGGCGCAAGGGCGCCGGGTTAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCGGCCCTGAGCTGctg                                                                                                                                   <  7:129414/149‑3 (MQ=255)
          aCTGCGGCACGCGCTTGATGAAGAACTTCGGCTCCATCGGTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCggg                                                                                                                                  >  7:336757/1‑149 (MQ=255)
              cGGCACGCGCTTGATGAAGAACTTCGGCTCCATCGGTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGca                                                                                                                              >  2:173560/1‑149 (MQ=255)
              cGGCACGCGCTTGATGAAGAACTTCGGCTCCATCGGTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGc                                                                                                                               <  8:47689/148‑1 (MQ=255)
              cGGCACGCGCTTGATGAAGAACTTCGGCTCCATCGGTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGc                                                                                                                               <  8:155378/148‑1 (MQ=255)
              cGGCACGCGCTTGATGAAGAACTTCGGCTCCATCGGTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGc                                                                                                                               <  8:238992/148‑1 (MQ=255)
                  aCGCGCTTGATGAAGAACTTCGGCTCCATCGGTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGcaccac                                                                                                                          >  3:306327/1‑149 (MQ=255)
                        ttGATGAAGAACTTCGGCTCCATCGGTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAAt                                                                                                                    >  6:389413/1‑149 (MQ=255)
                          gatgaAGAACTTCGGCTCCATCGGTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGTCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAAt                                                                                                                    <  8:293393/147‑1 (MQ=255)
                            tgaAGAACTTCGGCTCCATCGGTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAAc                                                                                                                <  7:217063/149‑1 (MQ=255)
                              aagaaCTTCGGCTCCATCGGTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGTCAAAGCACATCACCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTg                                                                                                              >  4:338903/1‑149 (MQ=255)
                              aagaaCTTCGGCTCCATCGGTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTg                                                                                                              <  5:410174/149‑1 (MQ=255)
                                       ggCTCCATCGGTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCAc                                                                                                     >  4:241425/1‑149 (MQ=255)
                                        gCTCCATCGGTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCAc                                                                                                     >  4:106659/1‑148 (MQ=255)
                                          tCCATCGGTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTACGGGTGCACCACCAGAATCAACTGGc                                                                                                            >  7:310923/1‑139 (MQ=255)
                                          tCCATCGGTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTACGGGTGCACCACCAGAATCAACTGGc                                                                                                            <  8:310923/139‑1 (MQ=255)
                                             aTCGGTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGccc                                                                                               <  3:384899/149‑1 (MQ=255)
                                                ggTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAg                                                                                            <  3:106659/149‑1 (MQ=255)
                                                 gTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCCGCCCCAgg                                                                                           >  8:368195/1‑149 (MQ=255)
                                                    ccGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTAc                                                                                        <  4:306327/149‑1 (MQ=255)
                                                       ggCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAg                                                                                     >  6:374926/1‑149 (MQ=255)
                                                            aaGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCCCCACCAGAATCCACTGGCCAAGCACCAGCCCCAGGTACGAGGCAAt                                                                                >  2:272401/1‑149 (MQ=255)
                                                               ggCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCAACAGCCCCAGGTACGAGGCAATCAt                                                                             <  1:290186/149‑1 (MQ=255)
                                                                  gcCGTGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTa                                                                          <  2:96251/149‑1 (MQ=255)
                                                                       gTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGc                                                                     >  6:378510/1‑149 (MQ=255)
                                                                           aTACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGaa                                                                 <  3:241425/149‑1 (MQ=255)
                                                                               gCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACAcc                                                             <  8:379914/149‑1 (MQ=255)
                                                                                   ggTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCgcgc                                                         >  4:336806/1‑149 (MQ=255)
                                                                                        tCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCgg                                                    <  8:336757/149‑1 (MQ=255)
                                                                                          gCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTg                                                  <  5:378510/149‑1 (MQ=255)
                                                                                            cACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGc                                                                                                 >  1:221332/1‑100 (MQ=255)
                                                                                            cACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGc                                                                                                 <  2:221332/100‑1 (MQ=255)
                                                                                             aCGGGCACCAGGCACAGGTCAAAGCACATCACCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCtt                                               <  3:338903/149‑1 (MQ=255)
                                                                                                     cAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGa                                                                                      >  1:6993/1‑102 (MQ=255)
                                                                                                     cAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGa                                                                                      <  2:6993/102‑1 (MQ=255)
                                                                                                     cAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCtt                                               <  4:335969/141‑1 (MQ=255)
                                                                                                     cAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCtt                                               >  3:335969/1‑141 (MQ=255)
                                                                                                     cAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCAATGGACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCgg                                       >  7:149568/1‑149 (MQ=255)
                                                                                                        gCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCt                                    <  8:14384/149‑1 (MQ=255)
                                                                                                             gggCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCTGCTCa                               >  8:201072/1‑149 (MQ=255)
                                                                                                              ggCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAAc                                                                                                                >  8:98708/1‑67 (MQ=255)
                                                                                                              ggCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAAc                                                                                                                <  7:98708/67‑1 (MQ=255)
                                                                                                               gCAAAGCACATCGGCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCTGCTCAtt                             <  6:342723/149‑1 (MQ=255)
                                                                                                                      acaTCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCTGCTCATTGAGc                         <  7:188346/146‑1 (MQ=255)
                                                                                                                      acaTCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCTGCTCATTGAGc                         >  8:188346/1‑146 (MQ=255)
                                                                                                                      acaTCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCTGCTCATTGAGCCAg                      <  7:201072/149‑1 (MQ=255)
                                                                                                                             caccaACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCt                                    <  8:134139/128‑1 (MQ=255)
                                                                                                                             caccaACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCt                                    >  7:134139/1‑128 (MQ=255)
                                                                                                                               ccaACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCTGCTCATTGAGCCAGCTCTCGATg             >  7:333166/1‑149 (MQ=255)
                                                                                                                                caACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCTGCTCATTGAGCCAGCTCTCGATGa            <  5:374926/149‑1 (MQ=255)
                                                                                                                                  aCATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCTGCTCATTGAGCCAGCTCTCGATGAcc          <  4:250299/149‑1 (MQ=255)
                                                                                                                                     tCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCTGCTCATTGAGCCAGCTCTCGATGACCAt        >  3:257890/1‑148 (MQ=255)
                                                                                                                                     tCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCTGCTCATTGAGCCAGCTCTCGATGACCAt        <  4:257890/148‑1 (MQ=255)
                                                                                                                                     cccgcagcTCGGCCCTGAGCTGCTGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCTGCTCATTGAGCCAGCTCTCGATGACCATg       >  1:96048/4‑149 (MQ=255)
                                                                                                                                     cccgcagcTCGGCCCTGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCTGCTCATTGAGc                         >  2:398552/4‑131 (MQ=255)
                                                                                                                                     cccgcagcTCGGCCCTGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCTGCTCATTGAGc                         <  1:398552/128‑1 (MQ=255)
                                                                                                                                     cccgcagcTCGGCCCTGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCTGCTCATTGAGCCAGCtctc                 >  3:145554/4‑139 (MQ=255)
                                                                                                                                     cccgcagcTCGGCCCTGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCTGCTCATTGAGCCAGCtctc                 <  4:145554/136‑1 (MQ=255)
                                                                                                                                      ccgcagcTCGGCCCTGAGCTGCTGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCTGCTCATTGAGCCAGCTCTCGATGACCATg       >  2:97527/3‑148 (MQ=255)
                                                                                                                                        gcagcTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCTGCTCATTGAGCCAGCTCTCGATGAcc          >  4:188172/1‑143 (MQ=255)
                                                                                                                                        gcagcTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCTGCTCATTGAGCCAGCTCTCGATGAcc          <  3:188172/143‑1 (MQ=255)
                                                                                                                                          agcTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCTGCTCATTGAGCCAGCTCTCGATGACCATGTATTg  <  5:389413/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                          
GTACTGAGCGACTGCGGCACGCGCTTGATGAAGAACTTCGGCTCCATCGGTGCCGGGCGCAAGGGCGCCGGGTGAATACGCCGGGTCATCGCCACGGGCACCAGGCACAGGGCAAAGCACATCGCCACCAACATCAGCAGCTCAGGGCCGAGCTGCGGGTGCACCACCAGAATCAACTGGCCAAGCACCAGCCCCAGGTACGAGGCAATCATGTAGCCGCTGAACACCGCGCCCCGGTGCTTCACATCGGCCTGCTCATTGAGCCAGCTCTCGATGACCATGTATTG  >  NC_002947/4989142‑4989428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑