Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,054,819 A→G 5.8% P190P (CCT→CCC)  PP_4454 ← opine ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,054,8190AG 5.8% 207.1 / 2.7 69P190P (CCT→CCC) PP_4454opine ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (31/34);  new base G (2/2);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.80e-01

CCCCCAGGTCGGGGCGGGCGGTGGGACACCCAAGCCGATGAAGCTCAGCGAAGCTTCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGCGATCTCGGTGATGGCGATAGCGATGATGAGATTTTCAAGACTCGCGCCGAGCATGGCG  >  NC_002947/5054672‑5054966
                                                                                                                                                   |                                                                                                                                                    
cccccAGGTCGGGGCGGGCGGTGGGACACCCAAGCCGATGAAGCTCAGCGAAGCTTCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAg                                                                                                                                                     >  8:370593/1‑149 (MQ=255)
 ccccAGGTCGGGGCGGGCGGTGGGACACCCAAGCCGATGAAGCTCAGCGAAGCTTCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACCGTCATCAGTCGACCAgg                                                                                                                                                    >  6:328027/1‑149 (MQ=255)
 ccccAGGTCGGGGCGGGCGGTGGGACACCCAAGCCGATGAAGCTCAGCGAAGCTTCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAgg                                                                                                                                                    <  5:178345/149‑1 (MQ=255)
  cccAGGTCGGGGCGGGCGGTGGGACACCCAAGCCGATGAAGCTCAGCGAAGCTTCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAggg                                                                                                                                                   <  7:189838/149‑1 (MQ=255)
   cccggTCGGGGCGGGCGGTGGGACACCCAAGCCGATGAAGCTCAGCGAAGCTTCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGc                                                                                                                                                  <  4:260703/146‑1 (MQ=255)
   ccAGGTCGGGGCGGGCGGTGGGACACCCAAGCCGATGAAGCTCAGCGAAGCTTCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGc                                                                                                                                                  >  6:365436/1‑149 (MQ=255)
   ccAGGTCGGGGCGGGCGGTGGGACACCCAAGCCGATGAAGCTCAGCGAAGCTTCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGc                                                                                                                                                  >  1:394356/1‑149 (MQ=255)
        tCGGGGCGGGCGGTGGGACACCCAAGCCGATGAAGCTCAGCGAAGCTTCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATAc                                                                                                                                             >  4:249415/1‑149 (MQ=255)
         cGGGGCGGGCGGTGGGACACCCAAGCCGATGAAGCTCAGCGAAGCTTCTGTACGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATAcc                                                                                                                                            >  1:305698/1‑149 (MQ=255)
           gggctggcGGTGGGACACCCAAGCCGATGAAGCTCAGCGAAGCTTCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATAcccc                                                                                                                                          <  4:253919/149‑1 (MQ=255)
             gcgggcggTGGGACACCCAAGCCGATGAAGCTCAGCGAAGCTTCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCaa                                                                                                                                        <  7:370593/149‑1 (MQ=255)
                     tGGGACACCCAAGCCGATGAAGCTCAGCGAAGCTTCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGc                                                                                                                                <  2:305698/149‑1 (MQ=255)
                          cacCCAAGCCGATGAAGCTCAGCGAAGCTTCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGc                                                                                                                            >  8:334650/1‑148 (MQ=255)
                             ccAAGCCGATGAAGCTCAGCGAAGCTTCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACgg                                                                                                                                 <  1:27083/140‑1 (MQ=255)
                             ccAAGCCGATGAAGCTCAGCGAAGCTTCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACgg                                                                                                                                 >  2:27083/1‑140 (MQ=255)
                             ccAAGCCGATGAAGCTCAGCGAAGCTTCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑Tc                                                                                                                        >  3:124170/1‑148 (MQ=255)
                                          gCTCAGCGAAGCTTCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAAGGAACGGCCAGCC‑TCGACGAAATCGCgtt                                                                                                          >  5:238574/1‑149 (MQ=255)
                                          gCTCAGCGAAGCTTCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCgtt                                                                                                          <  2:394356/149‑1 (MQ=255)
                                                   aGCTTCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTg                                                                                                 <  4:124170/149‑1 (MQ=255)
                                                   aGCTTCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTg                                                                                                 <  3:249415/149‑1 (MQ=255)
                                                      ttCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGTGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTa                                                                                              <  5:295353/149‑1 (MQ=255)
                                                      ttCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTa                                                                                              >  7:132937/1‑149 (MQ=255)
                                                       tCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTAt                                                                                             >  2:400338/1‑149 (MQ=255)
                                                        cTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCCTTCGACGAAATCGCGTTGTTTGAGTGCTa                                                                                              <  1:231163/148‑1 (MQ=255)
                                                           tgCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTc                                                                                         >  1:209096/1‑149 (MQ=255)
                                                            gCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATg                                                                                            >  1:152028/1‑145 (MQ=255)
                                                            gCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATg                                                                                            <  2:152028/145‑1 (MQ=255)
                                                              gAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTCGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCAAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCg                                                                                        >  8:285513/1‑147 (MQ=255)
                                                                      gTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGc                                                                              >  7:133348/1‑149 (MQ=255)
                                                                      gTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGc                                                                              >  5:338809/1‑149 (MQ=255)
                                                                         gCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGAt                                                                           <  2:209096/149‑1 (MQ=255)
                                                                          ccATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTc                                                                                         <  6:367650/134‑1 (MQ=255)
                                                                          ccATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTc                                                                                         >  5:367650/1‑134 (MQ=255)
                                                                           cATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATAc                                                                         <  1:400338/149‑1 (MQ=255)
                                                                           cATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATAc                                                                         <  8:132937/149‑1 (MQ=255)
                                                                            aTCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACg                                                                        <  7:285513/149‑1 (MQ=255)
                                                                             tCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACg                                                                        >  5:231928/1‑148 (MQ=255)
                                                                                acaGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGt                                                                                          <  8:196158/127‑1 (MQ=255)
                                                                                acaGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGt                                                                                          >  7:196158/1‑127 (MQ=255)
                                                                                   gagCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACaaaa                                                                 <  2:201754/149‑1 (MQ=255)
                                                                                   gagCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACaaaa                                                                 <  6:238574/149‑1 (MQ=255)
                                                                                        ccGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGc                                                            <  5:365436/149‑1 (MQ=255)
                                                                                          gAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGCGa                                                          >  5:168726/1‑149 (MQ=255)
                                                                                                 agaACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGCGATCTCGGt                                                   <  6:231928/149‑1 (MQ=255)
                                                                                                   aaCTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATg                                                                                            >  2:315716/1‑106 (MQ=255)
                                                                                                   aaCTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGCGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATg                                                                                            <  1:315716/106‑1 (MQ=255)
                                                                                                             aTCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGCGATCTCGGTGATGGCGATAGc                                       <  6:204269/149‑1 (MQ=255)
                                                                                                                aTGTTGTGCAAGATATGCACAGTCATCATTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGATTGCTATGGTCGGAGGCCGGGCGATACGAACAAAAGGTGCGATCTCGGTGATGGCGATAGCga                                     <  6:351911/148‑1 (MQ=255)
                                                                                                                aTGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGCGATCTCGGTGATGGCGATAGCgat                                    <  8:133348/149‑1 (MQ=255)
                                                                                                                   ttGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGCGATCTCGGTGATGGCGATAGCgatgat                                 <  1:393921/149‑1 (MQ=255)
                                                                                                                      ggCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGCGATCTCGGTGATGGCGATAGCgatgat                                 >  5:372825/1‑146 (MQ=255)
                                                                                                                      ggCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGCGATCTCGGTGATGGCGATAGCGAtgatga                               <  8:269759/148‑1 (MQ=255)
                                                                                                                           gATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGCGATCTCGGTGATGGCGATAGCGATGATGAGAtttt                         <  7:334650/149‑1 (MQ=255)
                                                                                                                            atatGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGCGATCTCGGTGATGGCGATAGCGATGATGAGAtttt                         >  3:37607/1‑148 (MQ=255)
                                                                                                                                gCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGCGATCTCGGTGATGGCGATAGCGATGATGAGATTTTCAAGa                    >  2:178776/1‑149 (MQ=255)
                                                                                                                                  acaGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGCGATCTCGGTGATGGCGATAGCGATGATGAGATTTTCAAGACt                  >  1:209475/1‑149 (MQ=255)
                                                                                                                                      tcatcaGTCGACCGGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGt                                                              <  3:188800/101‑1 (MQ=255)
                                                                                                                                      tcatcaGTCGACCGGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGt                                                              >  4:195012/1‑101 (MQ=255)
                                                                                                                                      tcatcaGTCGACCGGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGt                                                              >  4:188800/1‑101 (MQ=255)
                                                                                                                                      tcatcaGTCGACCGGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGt                                                              <  3:195012/101‑1 (MQ=255)
                                                                                                                                        atcaGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGCGATCTCGGTGATGGCGATAGCGATGATGAGATTTTCAAGACTCGCGcc            >  1:117362/1‑149 (MQ=255)
                                                                                                                                        atcaGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGCGATCTCGGTGATGGCGATAGCGATGATGAGATTTTCAAGACTCGCGcc            >  8:67383/1‑149 (MQ=255)
                                                                                                                                            gTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGc                                                            >  7:305984/1‑97 (MQ=255)
                                                                                                                                            gTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGc                                                            <  8:305984/97‑1 (MQ=255)
                                                                                                                                            gTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGCGATCTCGGTGATGGCGATAGCGATGATGAGATTTTCAAGACTCGCGCCGAGc        <  2:117362/149‑1 (MQ=255)
                                                                                                                                             tCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGCGATCTCGGTGATGGCGATAGCGATGATGAGATTTTCAAGACTCGCGCCGAGCa       >  1:7787/1‑149 (MQ=255)
                                                                                                                                               gACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGCGATCTCGGTGATGGCGATAGCGATGATGAGATTTTCAAGACTCGCGCCGAGCATg     >  5:3548/1‑149 (MQ=255)
                                                                                                                                                 ccAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGCGATCTCGGTGATGGCGATAGCGATGATGAGATTTTCAAGACTCGCGCCGAGCATggc   <  6:338809/149‑1 (MQ=255)
                                                                                                                                                  cAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGCGATCTCGGTGATGGCGATAGCGATGATGAGATTTTCAAGACTCGCGCCGAGCATggcg  <  6:168726/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCCCCAGGTCGGGGCGGGCGGTGGGACACCCAAGCCGATGAAGCTCAGCGAAGCTTCTGTGCGAATGGCCGTCGCCATCCACAGAGCACCGAGTACGAGAACTTCCGAGATCATGTTGGGCAAGATATGCACAGTCATCAGTCGACCAGGGCCATACCCCAACGAACGGCCAGCC‑TCGACGAAATCGCGTTGTTTGAGTGCTATGGTCGGAGCCCGGGCGATACGAACAAAAGGTGCGATCTCGGTGATGGCGATAGCGATGATGAGATTTTCAAGACTCGCGCCGAGCATGGCG  >  NC_002947/5054672‑5054966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑