Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	346,118	G→A	6.2%	N576N (AAC→AAT) ‡	PP_0287 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	346,118	0	G	A	6.2%	241.9 / 4.0	80	N576N (AAC→AAT) ‡	PP_0287	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (40/35);  new base A (2/3);  total (42/38)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01

GACGCCATTGCGTACCACCAGGCTGCCGCGCAGCTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGACCGGCGGCGTCTGTTCCTTGCCTTCGGTCTTGATGAAGTGTTCCACCG  >  NC_002947/345971‑346266                                                                                                                                                    |                                                                                                                                                     gACGCCATTGCGTACCACCAGGCTGCCGCGCAGCTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGt                                                                                                                                                     >  1:338193/1‑149 (MQ=255)   cGCCATTGCGTACCACCAGGCTGCCGCGCAGCTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTg                                                                                                                                                   <  7:116470/149‑1 (MQ=255)    gCCATTGCGTACCACCAGGCTGCCGCGCAGCTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGa                                                                                                                                                  <  2:26360/149‑1 (MQ=255)       aTTGCGTACCACCAGGCTGCCGCGCAGCTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCAGCGTTGATgg                                                                                                                                               >  1:276069/1‑149 (MQ=255)           cGTACCACCAGGCTGCCGCGCAGCTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCAACGATGGCgtg                                                                                                                                           <  6:365326/149‑3 (MQ=255)            gTACCACCAGGCTGCCGCGCAGCTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAg                                                                                                                                          >  4:133382/1‑149 (MQ=255)             tACCACCAGGCTGCCGCGCAGCTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGt                                                                                                                                         >  5:69934/1‑149 (MQ=255)               ccaccaGGCTGCCGCGCAGCTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGttt                                                                                                                                       <  7:86312/149‑1 (MQ=255)               ccaccaGGCTGCCGCGCAGCTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCAACGATGGCgtgctg                                                                                                                                        <  5:179296/148‑6 (MQ=255)                caccaGGCTGCCGCGCAGCTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTg                                                                                                                                      >  7:198620/1‑149 (MQ=255)                 accaGGCTGCCGCGCAGCTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTgc                                                                                                                                     >  4:76158/1‑149 (MQ=255)                  ccaGGCTGCCGCGCAGCTCCTGGAAGGGGGTGTCCTTGCCGGGTGGTTCGCTGCTGAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGNCCATCTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGa                                                                                                                                                  <  1:289673/135‑1 (MQ=255)                  ccaGGCTGCCGCGCAGCTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTgcg                                                                                                                                    <  5:35378/149‑1 (MQ=255)                  ccaGGCTGCCGCGCAGCTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTgcg                                                                                                                                    >  6:24408/1‑149 (MQ=255)                  ccaGGCTGCCGCGCAGCTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTgcg                                                                                                                                    >  6:30274/1‑149 (MQ=255)                  ccaGGCTGCCGCGCAGCTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACAAGCACGCCATCGTTGa                                                                                                                                                  >  2:289673/1‑135 (MQ=255)                    aGGCTGCCGCGCAGCTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTTCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCt                                                                                                                                  >  1:308657/1‑149 (MQ=255)                     ggCTGCCGCGCAGCTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTg                                                                                                                                 >  2:123691/1‑149 (MQ=255)                       cTGCCGCGCAGCTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGcc                                                                                                                               <  2:338193/149‑1 (MQ=255)                       cTGCCGCGCAGCTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGcc                                                                                                                               <  4:246593/149‑1 (MQ=255)                             cgcAGCTACTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCAAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAg                                                                                                                         >  4:291167/1‑149 (MQ=255)                                  cTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATgg                                                                                                                                               >  1:264814/1‑122 (MQ=255)                                  cTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATgg                                                                                                                                               <  2:264814/122‑1 (MQ=255)                                    ccTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTc                                                                                                                   >  6:344866/1‑148 (MQ=255)                                     cTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGa                                                                                                                 >  6:308207/1‑149 (MQ=255)                                      tGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGAc                                                                                                                >  4:31715/1‑149 (MQ=255)                                      tGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGAGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGATGTTCCAGGTTGGCATTGACCAGCACTCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTAGa                                                                                                                 >  8:179726/1‑148 (MQ=255)                                       ggAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGAc                                                                                                                >  2:329744/1‑148 (MQ=255)                                               gtgtCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCttt                                                                                                       >  2:124207/1‑149 (MQ=255)                                                    cTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTgg                                                                                                   <  5:344866/148‑1 (MQ=255)                                                     ttGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCt                                                                                                 >  2:389120/1‑149 (MQ=255)                                                       gCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCt                                                                                                 <  3:76158/147‑1 (MQ=255)                                                        ccGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTgg                                                                                               >  6:71342/1‑147 (MQ=255)                                                            gTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCg                                                                                          <  6:69934/149‑1 (MQ=255)                                                            gTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCg                                                                                          <  4:336299/149‑1 (MQ=255)                                                             tGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCgg                                                                                         >  7:107713/1‑149 (MQ=255)                                                              ggTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGTACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGt                                                                                        >  4:127744/1‑149 (MQ=255)                                                               gTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTTGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCTGTCAGGGTGTTGACCAGTGCTTTCTGGCTGTTACCGGTg                                                                                       <  7:179726/149‑1 (MQ=255)                                                                ttCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTgg                                                                                      <  5:86747/149‑1 (MQ=255)                                                                   gctgctCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCt                                                                                    <  5:24408/148‑1 (MQ=255)                                                                   gctgctCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCt                                                                                    <  5:30274/148‑1 (MQ=255)                                                                       ctCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTTCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGGCa                                                                                <  2:308657/148‑1 (MQ=255)                                                                       ctCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTc                                                                                 <  4:42918/147‑1 (MQ=255)                                                                       ctCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTc                                                                                 <  5:308207/147‑1 (MQ=255)                                                                       ctCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCa                                                                                <  2:406818/148‑1 (MQ=255)                                                                          aGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGAt                                                                             >  8:202080/1‑148 (MQ=255)                                                                            cGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCAc                                                                          <  8:107713/149‑1 (MQ=255)                                                                             gTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACt                                                                         <  2:106055/149‑1 (MQ=255)                                                                              tCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTg                                                                        <  1:389120/149‑1 (MQ=255)                                                                                     aTTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTggtcaggg                                                                 >  4:324607/1‑149 (MQ=255)                                                                                                  aTGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCtt                                                    <  8:198620/149‑1 (MQ=255)                                                                                                     gCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGAc                                                 <  3:324607/149‑1 (MQ=255)                                                                                                       ttGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGc                                                                                                  >  1:367894/1‑98 (MQ=255)                                                                                                       ttGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGc                                                                                                  <  2:367894/98‑1 (MQ=255)                                                                                                       ttGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGTTCAGCAGGCCCTTGACcg                                               >  7:42009/1‑149 (MQ=255)                                                                                                       ttGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGACcg                                               >  8:292963/1‑149 (MQ=255)                                                                                                          gCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGAc                                                 <  5:12842/144‑1 (MQ=255)                                                                                                          gCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGAc                                                 >  6:12842/1‑144 (MQ=255)                                                                                                            acaACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGACCggcggc                                          >  4:387312/1‑149 (MQ=255)                                                                                                                           ggTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTg                                                                        <  1:298881/104‑1 (MQ=255)                                                                                                                           ggTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTg                                                                        >  2:298881/1‑104 (MQ=255)                                                                                                                             ttGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGACCGGCGGCGTCTGTTCCTTGCCTTc                         <  3:127744/149‑1 (MQ=255)                                                                                                                               ggCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGGCAGGGTCAGCAGGCCCTTGACCGGCGGCGTCTGTTCCTTTCCTTCg                        <  5:71342/148‑1 (MQ=255)                                                                                                                                gCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCTCTGGTCAGGGTCAGCAGGCCCTTGACCGGCGGCGTCTGTTCCTTGCCTTCgg                       <  2:276069/148‑1 (MQ=255)                                                                                                                                  aTTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGACCGGCGGCGTCTGTTCCTTGCCTTCGGTCt                    >  5:149605/1‑149 (MQ=255)                                                                                                                                      aCCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGACCGGCGGCGTCTGTTCCTTGCCTTCGGTCTtgat                >  3:128882/1‑149 (MQ=255)                                                                                                                                      aCCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGACCGGCGGAGGCTTGTCCTTGCATTCGGTCTtgat                >  8:334109/1‑149 (MQ=255)                                                                                                                                       ccAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGACCGGCGGCGTCTGTTCCTTGCCTTCGGTCTtgat                >  1:261771/1‑148 (MQ=255)                                                                                                                                        cAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGACCGGCGGCGTCTGTTCCTTGCCTTCGGTCTtgatga              <  7:292963/149‑1 (MQ=255)                                                                                                                                        cAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGACCGGCGGCGTCTGTTCCTTGCCTTCGGTCTtgatg               >  5:144011/1‑148 (MQ=255)                                                                                                                                        cAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACAGGTCAGGGTCAGCAGGCCCTTGACCGGCGGCGTCTGTTCCTTGCCTTCGGTCTtgatg               >  4:258644/1‑148 (MQ=255)                                                                                                                                         aGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGACCGGCGGCGTCTGTTCCTTGCCTTCGGTCTtgatg               >  1:145619/1‑147 (MQ=255)                                                                                                                                           ttcGCCATCAACGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGACCGGCGGCGTCTGTTCCTTGCCTTCGGTCTTGATGaa             >  7:336150/3‑147 (MQ=255)                                                                                                                                           ttcGCCATCAACGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGACCGGCGGCGTCTGTTCCTTGCCTTCGGTCTTGATGaa             <  8:336150/145‑1 (MQ=255)                                                                                                                                           ttcGCCATCAACGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGACCGGCGGCGTCTGTTCCTTGCCTTCGGTCTTGATGAAgt           >  2:292297/3‑149 (MQ=255)                                                                                                                                             cGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGACCGGCGGCGTCTGTTCCTTGCCTTCGGTCTTGATGAAgtgt         <  4:128882/149‑1 (MQ=255)                                                                                                                                              gCCATCGTTGTTGCCGAAGTTTGCGCTGGCGTTCAGTGTGTCGTCCTGTGGTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGACCGGCGGCGTCTGTTGCTTGCCTTCGGTCTTGATGAAGTGtt        <  3:291167/149‑1 (MQ=255)                                                                                                                                                cATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGACCGGCGGCGTCTGTTCCTTGCCTTCGGTCTTGATGAAGTGTTcc      >  2:305875/1‑149 (MQ=255)                                                                                                                                                 aTCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGACCGGCGGCGTCTGTTCCTTGCCTTCGGTCTTGATGAAGTGTTCCa     >  8:246028/1‑149 (MQ=255)                                                                                                                                                 aTCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGACCGGCGGCGTCTGTTCCTTGCCTTCGGTCTTGATGAAGTGTTCCa     <  2:261771/149‑1 (MQ=255)                                                                                                                                                    gTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGACCGGCGGCGTCTGTTCCTTGCCTTCGGTCTTGATGAAGTGTTCCACCg  <  7:246028/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GACGCCATTGCGTACCACCAGGCTGCCGCGCAGCTCCTGGAAGGGGGTGTCCTTGCCGCGTGGTTCGCTGCTCAGCGTCTTGCGATTGAGCGTGGCGATGGCTTGGCACAACTGCTGTTCCAGGTTGGCATTGACCAGCACGCCATCGTTGATGGCGAAGTTTGCGCTGCCGTTCAGGGTGTCGACCAGTGCTTTCTGGCTGTTACCGGTGGCTGTCAGATCACTGGTCAGGGTCAGCAGGCCCTTGACCGGCGGCGTCTGTTCCTTGCCTTCGGTCTTGATGAAGTGTTCCACCG  >  NC_002947/345971‑346266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑