Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,096,464 A→G 10.2% V101A (GTC→GCC) ‡ argT ← lysine/arginine/ornithine ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,096,4640AG10.2% 126.0 / 4.5 49V101A (GTC→GCC) ‡argTlysine/arginine/ornithine ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (18/26);  new base G (3/2);  total (21/28)
Fisher's exact test for biased strand distribution p-value = 6.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAGGCCGTCGAACTCCTGCTCGACCCAGGTACACTTGGTTTTCATCTCTTCGCACAGCGCGTTGCCGATATC  >  NC_002947/5096321‑5096612
                                                                                                                                                  |                                                                                                                                                    
cTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTcgaccgac                                                                                                                                                 <  4:108073/149‑1 (MQ=255)
   ccTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTTGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGGTCGActt                                                                                                                                               >  3:260263/1‑148 (MQ=255)
    cTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGActtc                                                                                                                                              <  8:226506/148‑1 (MQ=255)
     ttGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGActtatt                                                                                                                                            >  7:266604/1‑149 (MQ=255)
        gCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTTGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGGTCGActtcaccaa                                                                                                                                         <  4:260263/149‑6 (MQ=255)
        gCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCg                                                                                                                                         <  6:106254/149‑1 (MQ=255)
         cGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCgg                                                                                                                                        <  3:185145/149‑1 (MQ=255)
              cGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATc                                                                                                                                   >  5:286519/1‑149 (MQ=255)
               ggTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCg                                                                                                                                  <  4:15805/149‑1 (MQ=255)
                gTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTcgaccgac                                                                                                                                                 >  4:313797/1‑133 (MQ=255)
                gTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTcgaccgac                                                                                                                                                 <  3:313797/133‑1 (MQ=255)
                gTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGGTCGActtcac                                                                                                                                            <  8:256411/138‑3 (MQ=255)
                gTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGGTCGActtcac                                                                                                                                            >  7:256411/1‑136 (MQ=255)
                gTCGTGAATGGAGCCGAGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCgg                                                                                                                                 >  8:29731/1‑149 (MQ=255)
                    ttaaTGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGAt                                                                                                                             <  3:60672/147‑1 (MQ=255)
                      aaTGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATc                                                                                                                            <  4:203488/148‑1 (MQ=255)
                      aaTGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATc                                                                                                                            >  2:222106/1‑148 (MQ=255)
                          gAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACAt                                                                                                                       <  3:354342/149‑1 (MQ=255)
                           aGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATg                                                                                                                      <  3:387744/149‑1 (MQ=255)
                             ccGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGga                                                                                                                    <  3:88420/149‑1 (MQ=255)
                               gcgcTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGAc                                                                                                                         >  1:375354/1‑142 (MQ=255)
                               gcgcTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGgacg                                                                                                                  <  2:310373/149‑1 (MQ=255)
                               gcgcTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGgac                                                                                                                   >  3:63273/1‑148 (MQ=255)
                                    gCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAg                                                                                                              >  3:183244/1‑148 (MQ=255)
                                      aCGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGGGATCGACATGGACGACAGGAt                                                                                                           <  6:293327/149‑1 (MQ=255)
                                           gATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGCTAAGCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATgg                                                                                                         >  1:125520/1‑149 (MQ=255)
                                                 cttGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGGGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCg                                                                                                    <  6:367436/145‑1 (MQ=255)
                                                         tCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCa                                                                                          <  4:63273/147‑1 (MQ=255)
                                                            gTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTc                                                                                     <  7:29731/149‑1 (MQ=255)
                                                             ttCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGGGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACttt                                                                                      >  7:88077/1‑147 (MQ=255)
                                                                   cAGGATGTCGCTGACGGTTGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGTTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCg                                                                              <  6:322511/149‑1 (MQ=255)
                                                                    aGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCgg                                                                             <  3:61063/149‑1 (MQ=255)
                                                                        tGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGAt                                                                          >  2:43047/1‑148 (MQ=255)
                                                                          tCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAg                                                                       >  1:132752/1‑149 (MQ=255)
                                                                            gCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAgg                                                                      <  2:258833/148‑1 (MQ=255)
                                                                            gCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAGGc                                                                     >  5:359666/1‑149 (MQ=255)
                                                                                          cGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAGGCCGTCGAACTCCTGc                                                       <  8:88077/149‑1 (MQ=255)
                                                                                                      ccAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAGGCCGTCGAACTCCTGCTCGACCCAGGTa                                           >  4:382086/1‑149 (MQ=255)
                                                                                                          gcgc‑‑‑gcCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGATGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAGGCCGTCGAACTCCTGCTCGACCCAGGTACACt                                       >  1:114484/1‑149 (MQ=255)
                                                                                                           cgc‑‑‑gcCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAGGCCGTCGAACTCCTGCTCGACCCAGGTACACtt                                      <  6:359666/149‑1 (MQ=255)
                                                                                                                      aGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAGGCCGTCGAACTCCTGCTCGACCCAGGTACACTTGGTTTTCa                              <  2:34341/149‑1 (MQ=255)
                                                                                                                       gTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAGGCCGTCGAACTCCTGCTCGACCCAGGTACACTTGGTTTTCAt                             <  1:222106/149‑1 (MQ=255)
                                                                                                                         cAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCTGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAGGCCGTCGAACTCCTGCTCGACCCAGGTACACTTGGTTTTCAtct                           <  3:13320/149‑1 (MQ=255)
                                                                                                                                   cgcTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAGGCCGTCGAACTCCTGCTCGACCCAGGTACACTTGGTTTTCATCTCTTCGCACAg                 >  6:319515/1‑149 (MQ=255)
                                                                                                                                    gcTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAGGCCGTCGAACTCCTGCTCGACCCAg                                              <  7:112419/119‑1 (MQ=255)
                                                                                                                                    gcTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAGGCCGTCGAACTCCTGCTCGACCCAg                                              >  8:112419/1‑119 (MQ=255)
                                                                                                                                     ccgcaagAAGTCGGTCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAGGCCGTCGAACTCCTGCTCGACCCAGGTACACTTGGTTTTCATCTCTTCGCACAgcg               >  6:351840/7‑149 (MQ=255)
                                                                                                                                      gtGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAGGCCGTCGAACTCCTGCTCGACCCAGGTACACTTGGTTTTCATCTCTTCGCACAgcgc              <  7:185852/148‑1 (MQ=255)
                                                                                                                                        ggTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAGGCCGTCGAACTCCTGCTCGACCCAGGTACACTTGGTTTTCATCTCTTCGCACAGcgcg             <  2:132752/148‑1 (MQ=255)
                                                                                                                                        ggTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAGGCCGTCGAACTCCTGCTCGACCCAGGTACACTTGGTTTTCATCTCTTCGCACAGCGCGt            >  4:357258/1‑149 (MQ=255)
                                                                                                                                            aaGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAGGCCGTCGAACTCCTGCTCGACCCAGGTACACTTGGTTTTc                               >  7:97359/1‑126 (MQ=255)
                                                                                                                                            aaGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAGGCCGTCGAACTCCTGCTCGACCCAGGTACACTTGGTTTTc                               <  8:97359/126‑1 (MQ=255)
                                                                                                                                                  accgacTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAGGCCGTCGAACTCCTGCTCGACCCAGGTACACTTGGTTTTCATCTCTTCGCACAGCGCGTTGCCGATATc  <  5:319515/149‑1 (MQ=255)
                                                                                                                                                  accgacTTCTTGCGGTCATCGATGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAGGCCGTCGAACTCCTGCTCGACCCAGGTACACTTGGTTTTCATCTCTTCGCACAGCGCGTTGCCGATATc  <  2:114484/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CTTCCTTGGCGAAGCGGTCGTGAATGGAGCCGCGCTGCACGCCGATCTTCTTGCCTTTCAGTTCGTCCAGGCTGTCGCTGACGGTGGTGCCGTCCTTCATGACCAGGCGC‑‑‑GCCGGAGTCAGGTAGTAGCGCTTGGTGAAGTCGACCGACTTCTTGCGGTCATCGGTGATCGACATGGACGACAGGATGGCGTCGATCTTGCGCACTTTCAGCGCCGGGATCAGGCCGTCGAACTCCTGCTCGACCCAGGTACACTTGGTTTTCATCTCTTCGCACAGCGCGTTGCCGATATC  >  NC_002947/5096321‑5096612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑